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Trace element landscape of resting and activated human neutrophils on the sub-micrometer level

Maria Joanna Niemiec, Björn De Samber (UGent) , Jan Garrevoet (UGent) , Eva Vergucht (UGent) , Bart Vekemans (UGent) , Riet De Rycke (UGent) , Erik Björn, Linda Sandblad, Gerd Wellenreuther, Gerald Falkenberg, et al.
(2015) METALLOMICS. 7(6). p.996-1010
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Abstract
Every infection is a battle for trace elements. Neutrophils migrate first to the infection site and accumulate quickly to high numbers. They fight pathogens by phagocytosis and intracellular toxication. Additionally, neutrophils form neutrophil extracellular traps (NETs) to inhibit extracellular microbes. Yet, neutrophil trace element characteristics are largely unexplored. We investigated unstimulated and phorbol myristate acetate-stimulated neutrophils using synchrotron radiation X-ray fluorescence (SR-XRF) on the sub-micron spatial resolution level. PMA activates pinocytosis, cytoskeletal rearrangements and the release of NETs, all mechanisms deployed by neutrophils to combat infection. By analyzing Zn, Fe, Cu, Mn, P, S, and Ca, not only the nucleus but also vesicular granules were identifiable in the elemental maps. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) revealed a neutrophil-specific composition of Zn, Fe, Cu, and Mn in comparison with J774 and HeLa cells, indicating a neutrophil-specific metallome complying with their designated functions. When investigating PMA-activated neutrophils, the SR-XRF analysis depicted typical subcellular morphological changes: the transformation of nucleus and granules and the emergence of void vacuoles. Mature NETs were evenly composed of Fe, P, S, and Ca with occasional hot spots containing Zn, Fe, and Ca. An ICP-MS-based quantification of NET supernatants revealed a NETosis-induced decrease of soluble Zn, whereas Fe, Cu, and Mn concentrations were only slightly affected. In summary, we present a combination of SR-XRF and ICP-MS as a powerful tool to analyze trace elements in human neutrophils. The approach will be applicable and valuable to numerous aspects of nutritional immunity.
Keywords
CELLS, METAL, ZINC, ICP-MS, CANDIDA-ALBICANS, EXTRACELLULAR TRAPS, PLASMA-MASS SPECTROMETRY, RADIATION, GROWTH, COPPER

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Citation

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MLA
Niemiec, Maria Joanna, et al. “Trace Element Landscape of Resting and Activated Human Neutrophils on the Sub-Micrometer Level.” METALLOMICS, vol. 7, no. 6, 2015, pp. 996–1010, doi:10.1039/C4MT00346B.
APA
Niemiec, M. J., De Samber, B., Garrevoet, J., Vergucht, E., Vekemans, B., De Rycke, R., … Urban, C. F. (2015). Trace element landscape of resting and activated human neutrophils on the sub-micrometer level. METALLOMICS, 7(6), 996–1010. https://doi.org/10.1039/C4MT00346B
Chicago author-date
Niemiec, Maria Joanna, Björn De Samber, Jan Garrevoet, Eva Vergucht, Bart Vekemans, Riet De Rycke, Erik Björn, et al. 2015. “Trace Element Landscape of Resting and Activated Human Neutrophils on the Sub-Micrometer Level.” METALLOMICS 7 (6): 996–1010. https://doi.org/10.1039/C4MT00346B.
Chicago author-date (all authors)
Niemiec, Maria Joanna, Björn De Samber, Jan Garrevoet, Eva Vergucht, Bart Vekemans, Riet De Rycke, Erik Björn, Linda Sandblad, Gerd Wellenreuther, Gerald Falkenberg, Peter Cloetens, Laszlo Vincze, and Constantin Felix Urban. 2015. “Trace Element Landscape of Resting and Activated Human Neutrophils on the Sub-Micrometer Level.” METALLOMICS 7 (6): 996–1010. doi:10.1039/C4MT00346B.
Vancouver
1.
Niemiec MJ, De Samber B, Garrevoet J, Vergucht E, Vekemans B, De Rycke R, et al. Trace element landscape of resting and activated human neutrophils on the sub-micrometer level. METALLOMICS. 2015;7(6):996–1010.
IEEE
[1]
M. J. Niemiec et al., “Trace element landscape of resting and activated human neutrophils on the sub-micrometer level,” METALLOMICS, vol. 7, no. 6, pp. 996–1010, 2015.
@article{5954416,
  abstract     = {{Every infection is a battle for trace elements. Neutrophils migrate first to the infection site and accumulate quickly to high numbers. They fight pathogens by phagocytosis and intracellular toxication. Additionally, neutrophils form neutrophil extracellular traps (NETs) to inhibit extracellular microbes. Yet, neutrophil trace element characteristics are largely unexplored. We investigated unstimulated and phorbol myristate acetate-stimulated neutrophils using synchrotron radiation X-ray fluorescence (SR-XRF) on the sub-micron spatial resolution level. PMA activates pinocytosis, cytoskeletal rearrangements and the release of NETs, all mechanisms deployed by neutrophils to combat infection. By analyzing Zn, Fe, Cu, Mn, P, S, and Ca, not only the nucleus but also vesicular granules were identifiable in the elemental maps. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) revealed a neutrophil-specific composition of Zn, Fe, Cu, and Mn in comparison with J774 and HeLa cells, indicating a neutrophil-specific metallome complying with their designated functions. When investigating PMA-activated neutrophils, the SR-XRF analysis depicted typical subcellular morphological changes: the transformation of nucleus and granules and the emergence of void vacuoles. Mature NETs were evenly composed of Fe, P, S, and Ca with occasional hot spots containing Zn, Fe, and Ca. An ICP-MS-based quantification of NET supernatants revealed a NETosis-induced decrease of soluble Zn, whereas Fe, Cu, and Mn concentrations were only slightly affected. In summary, we present a combination of SR-XRF and ICP-MS as a powerful tool to analyze trace elements in human neutrophils. The approach will be applicable and valuable to numerous aspects of nutritional immunity.}},
  author       = {{Niemiec, Maria Joanna and De Samber, Björn and Garrevoet, Jan and Vergucht, Eva and Vekemans, Bart and De Rycke, Riet and Björn, Erik and Sandblad, Linda and Wellenreuther, Gerd and Falkenberg, Gerald and Cloetens, Peter and Vincze, Laszlo and Urban, Constantin Felix}},
  issn         = {{1756-5901}},
  journal      = {{METALLOMICS}},
  keywords     = {{CELLS,METAL,ZINC,ICP-MS,CANDIDA-ALBICANS,EXTRACELLULAR TRAPS,PLASMA-MASS SPECTROMETRY,RADIATION,GROWTH,COPPER}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{996--1010}},
  title        = {{Trace element landscape of resting and activated human neutrophils on the sub-micrometer level}},
  url          = {{http://dx.doi.org/10.1039/C4MT00346B}},
  volume       = {{7}},
  year         = {{2015}},
}
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