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Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study

Nicky Van Der Vekens, Annelies Decloedt UGent, Dominique De Clercq UGent, Sofie Ven UGent and Gunther van Loon UGent (2015) VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT. 84(2). p.80-87
abstract
Brain natriuretic peptide (BNP) is used in human medicine for the diagnosis of congestive heart failure. Since BNP is species-specific and no equine assay is available, BNP has never been determined in horses. Because there is more than 90% homology between porcine and equine BNP, a porcine BNP enzyme-linked immunoassay (ELISA) was used in the present study to measure BNP in plasma of healthy horses (group 1; n=20), horses with cardiac disease without (group 2a; n=8) and with atrial dilatation (n=8), ventricular dilatation (n=1) or both (n=1) (group 2b; n=10). Samples were stored at -20 degrees C and -80 degrees C to study the influence of storage temperature. No significant differences were found between the BNP concentrations of group 1 (77.79; 37.20-513.36 pg/mL), group 2a (52.02; 24.69-268.37 pg/mL) or 2b (94.73; 42.88-470.66 pg/mL). Samples stored at -80 degrees C showed significantly (72.19, 24.69-513.36 pg/mL; P=0.001) higher concentrations than samples stored at -20 degrees C (47.35, 24.69-430.60 pg/mL). In this pilot study, it is suggested that the porcine BNP assay does not allow accurate detection of equine BNP. An equine specific BNP assay should be developed to study BNP concentrations in horses.
Please use this url to cite or link to this publication:
author
organization
alternative title
Analyse van equine BNP met een porciene BNP 'enzyme-linked immunoassay' : een pilootstudie
year
type
journalArticle (original)
publication status
published
subject
keyword
CONGESTIVE-HEART-FAILURE, NATRIURETIC-PEPTIDE CONCENTRATION, TROPONIN-I CONCENTRATIONS, NT-PROBNP, CARDIAC BIOMARKERS, TERMINAL PROBNP, ATRIAL, DISEASE, DOGS, HORSES
journal title
VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT
Vlaams Diergeneesk. Tijdschr.
volume
84
issue
2
pages
80 - 87
Web of Science type
Article
Web of Science id
000354073200003
JCR category
VETERINARY SCIENCES
JCR impact factor
0.203 (2015)
JCR rank
124/138 (2015)
JCR quartile
4 (2015)
ISSN
0303-9021
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5950761
handle
http://hdl.handle.net/1854/LU-5950761
alternative location
http://vdt.ugent.be/sites/default/files/03_0.pdf
date created
2015-05-06 15:56:09
date last changed
2016-12-19 15:47:23
@article{5950761,
  abstract     = {Brain natriuretic peptide (BNP) is used in human medicine for the diagnosis of congestive heart failure. Since BNP is species-specific and no equine assay is available, BNP has never been determined in horses. Because there is more than 90\% homology between porcine and equine BNP, a porcine BNP enzyme-linked immunoassay (ELISA) was used in the present study to measure BNP in plasma of healthy horses (group 1; n=20), horses with cardiac disease without (group 2a; n=8) and with atrial dilatation (n=8), ventricular dilatation (n=1) or both (n=1) (group 2b; n=10). Samples were stored at -20 degrees C and -80 degrees C to study the influence of storage temperature. No significant differences were found between the BNP concentrations of group 1 (77.79; 37.20-513.36 pg/mL), group 2a (52.02; 24.69-268.37 pg/mL) or 2b (94.73; 42.88-470.66 pg/mL). Samples stored at -80 degrees C showed significantly (72.19, 24.69-513.36 pg/mL; P=0.001) higher concentrations than samples stored at -20 degrees C (47.35, 24.69-430.60 pg/mL). In this pilot study, it is suggested that the porcine BNP assay does not allow accurate detection of equine BNP. An equine specific BNP assay should be developed to study BNP concentrations in horses.},
  author       = {Van Der Vekens, Nicky and Decloedt, Annelies and De Clercq, Dominique and Ven, Sofie and van Loon, Gunther},
  issn         = {0303-9021},
  journal      = {VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT},
  keyword      = {CONGESTIVE-HEART-FAILURE,NATRIURETIC-PEPTIDE CONCENTRATION,TROPONIN-I CONCENTRATIONS,NT-PROBNP,CARDIAC BIOMARKERS,TERMINAL PROBNP,ATRIAL,DISEASE,DOGS,HORSES},
  language     = {eng},
  number       = {2},
  pages        = {80--87},
  title        = {Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study},
  url          = {http://vdt.ugent.be/sites/default/files/03\_0.pdf},
  volume       = {84},
  year         = {2015},
}

Chicago
Van Der Vekens, Nicky, Annelies Decloedt, Dominique De Clercq, Sofie Ven, and Gunther van Loon. 2015. “Equine BNP Measurement Using a Porcine BNP Enzyme-linked Immunoassay: a Pilot Study.” Vlaams Diergeneeskundig Tijdschrift 84 (2): 80–87.
APA
Van Der Vekens, N., Decloedt, A., De Clercq, D., Ven, S., & van Loon, G. (2015). Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study. VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT, 84(2), 80–87.
Vancouver
1.
Van Der Vekens N, Decloedt A, De Clercq D, Ven S, van Loon G. Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study. VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT. 2015;84(2):80–7.
MLA
Van Der Vekens, Nicky, Annelies Decloedt, Dominique De Clercq, et al. “Equine BNP Measurement Using a Porcine BNP Enzyme-linked Immunoassay: a Pilot Study.” VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT 84.2 (2015): 80–87. Print.