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Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study

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Abstract
Brain natriuretic peptide (BNP) is used in human medicine for the diagnosis of congestive heart failure. Since BNP is species-specific and no equine assay is available, BNP has never been determined in horses. Because there is more than 90% homology between porcine and equine BNP, a porcine BNP enzyme-linked immunoassay (ELISA) was used in the present study to measure BNP in plasma of healthy horses (group 1; n=20), horses with cardiac disease without (group 2a; n=8) and with atrial dilatation (n=8), ventricular dilatation (n=1) or both (n=1) (group 2b; n=10). Samples were stored at -20 degrees C and -80 degrees C to study the influence of storage temperature. No significant differences were found between the BNP concentrations of group 1 (77.79; 37.20-513.36 pg/mL), group 2a (52.02; 24.69-268.37 pg/mL) or 2b (94.73; 42.88-470.66 pg/mL). Samples stored at -80 degrees C showed significantly (72.19, 24.69-513.36 pg/mL; P=0.001) higher concentrations than samples stored at -20 degrees C (47.35, 24.69-430.60 pg/mL). In this pilot study, it is suggested that the porcine BNP assay does not allow accurate detection of equine BNP. An equine specific BNP assay should be developed to study BNP concentrations in horses.
Keywords
CONGESTIVE-HEART-FAILURE, NATRIURETIC-PEPTIDE CONCENTRATION, TROPONIN-I CONCENTRATIONS, NT-PROBNP, CARDIAC BIOMARKERS, TERMINAL PROBNP, ATRIAL, DISEASE, DOGS, HORSES

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Citation

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Chicago
Van Der Vekens, Nicky, Annelies Decloedt, Dominique De Clercq, Sofie Ven, and Gunther van Loon. 2015. “Equine BNP Measurement Using a Porcine BNP Enzyme-linked Immunoassay: a Pilot Study.” Vlaams Diergeneeskundig Tijdschrift 84 (2): 80–87.
APA
Van Der Vekens, N., Decloedt, A., De Clercq, D., Ven, S., & van Loon, G. (2015). Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study. VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT, 84(2), 80–87.
Vancouver
1.
Van Der Vekens N, Decloedt A, De Clercq D, Ven S, van Loon G. Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study. VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT. 2015;84(2):80–7.
MLA
Van Der Vekens, Nicky, Annelies Decloedt, Dominique De Clercq, et al. “Equine BNP Measurement Using a Porcine BNP Enzyme-linked Immunoassay: a Pilot Study.” VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT 84.2 (2015): 80–87. Print.
@article{5950761,
  abstract     = {Brain natriuretic peptide (BNP) is used in human medicine for the diagnosis of congestive heart failure. Since BNP is species-specific and no equine assay is available, BNP has never been determined in horses. Because there is more than 90\% homology between porcine and equine BNP, a porcine BNP enzyme-linked immunoassay (ELISA) was used in the present study to measure BNP in plasma of healthy horses (group 1; n=20), horses with cardiac disease without (group 2a; n=8) and with atrial dilatation (n=8), ventricular dilatation (n=1) or both (n=1) (group 2b; n=10). Samples were stored at -20 degrees C and -80 degrees C to study the influence of storage temperature. No significant differences were found between the BNP concentrations of group 1 (77.79; 37.20-513.36 pg/mL), group 2a (52.02; 24.69-268.37 pg/mL) or 2b (94.73; 42.88-470.66 pg/mL). Samples stored at -80 degrees C showed significantly (72.19, 24.69-513.36 pg/mL; P=0.001) higher concentrations than samples stored at -20 degrees C (47.35, 24.69-430.60 pg/mL). In this pilot study, it is suggested that the porcine BNP assay does not allow accurate detection of equine BNP. An equine specific BNP assay should be developed to study BNP concentrations in horses.},
  author       = {Van Der Vekens, Nicky and Decloedt, Annelies and De Clercq, Dominique and Ven, Sofie and van Loon, Gunther},
  issn         = {0303-9021},
  journal      = {VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT},
  keyword      = {CONGESTIVE-HEART-FAILURE,NATRIURETIC-PEPTIDE CONCENTRATION,TROPONIN-I CONCENTRATIONS,NT-PROBNP,CARDIAC BIOMARKERS,TERMINAL PROBNP,ATRIAL,DISEASE,DOGS,HORSES},
  language     = {eng},
  number       = {2},
  pages        = {80--87},
  title        = {Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study},
  url          = {http://vdt.ugent.be/sites/default/files/03\_0.pdf},
  volume       = {84},
  year         = {2015},
}

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