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Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms

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Abstract
During a previous longitudinal study, performed on four farrow-to-finish farms (A to D), samples were taken from twelve sows, their offspring, and the environment on various occasions over six months to study the MRSA presence. During the present study, a selection of the obtained MRSA isolates were typed by multiple-locus variable-number tandem-repeat analysis (MLVA), Pulsed Field Gel Electrophoresis (PFGE), spa typing, and SCCmec typing to study the genetic diversity of LA-MRSA isolates and to determine possible MRSA sources for pig(let)s. PFGE, spa typing, and SCCmec typing revealed the presence of one or few dominant genotype(s) per farm. In contrast, 212 MLVA types were detected on the four farms, forming one cluster on farm A, three on farm B, four on farm C and two on farm D. The genotype, found on farm A was unique for this farm. Farms B, C and D shared one cluster. In general, MLVA types from these clusters were isolated from piglets, sows, and the environment on various sampling events. Piglets carried MLVA types both related and unrelated to their mother sows' MLVA types at farrowing and onwards. In conclusion, molecular typing revealed that within a farm one or a few dominant strain(s) are widespread. Potential MRSA sources for piglets were mother sows, the environment and other piglets.
Keywords
Genetic diversity, Pigs, Epidemiology, Molecular typing, LA-MRSA, RESISTANT STAPHYLOCOCCUS-AUREUS, FIELD GEL-ELECTROPHORESIS, TANDEM REPEAT ANALYSIS, RAPID IDENTIFICATION, NURSERY PIGLETS, PCR STRATEGY, MULTIPLEX, PIGS, MEC, COLONIZATION

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Chicago
Verhegghe, Marijke, Florence Crombé, Larissa J Pletinckx, Freddy Haesebrouck, Patrick Butaye, Lieve Herman, Marc Heyndrickx, and Geertrui Rasschaert. 2014. “Genetic Diversity of Livestock-associated MRSA Isolates Obtained from Piglets from Farrowing Until Slaughter Age on Four Farrow-to-finish Farms.” Veterinary Research 45.
APA
Verhegghe, M., Crombé, F., Pletinckx, L. J., Haesebrouck, F., Butaye, P., Herman, L., Heyndrickx, M., et al. (2014). Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms. VETERINARY RESEARCH, 45.
Vancouver
1.
Verhegghe M, Crombé F, Pletinckx LJ, Haesebrouck F, Butaye P, Herman L, et al. Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms. VETERINARY RESEARCH. 2014;45.
MLA
Verhegghe, Marijke, Florence Crombé, Larissa J Pletinckx, et al. “Genetic Diversity of Livestock-associated MRSA Isolates Obtained from Piglets from Farrowing Until Slaughter Age on Four Farrow-to-finish Farms.” VETERINARY RESEARCH 45 (2014): n. pag. Print.
@article{5942111,
  abstract     = {During a previous longitudinal study, performed on four farrow-to-finish farms (A to D), samples were taken from twelve sows, their offspring, and the environment on various occasions over six months to study the MRSA presence. During the present study, a selection of the obtained MRSA isolates were typed by multiple-locus variable-number tandem-repeat analysis (MLVA), Pulsed Field Gel Electrophoresis (PFGE), spa typing, and SCCmec typing to study the genetic diversity of LA-MRSA isolates and to determine possible MRSA sources for pig(let)s. PFGE, spa typing, and SCCmec typing revealed the presence of one or few dominant genotype(s) per farm. In contrast, 212 MLVA types were detected on the four farms, forming one cluster on farm A, three on farm B, four on farm C and two on farm D. The genotype, found on farm A was unique for this farm. Farms B, C and D shared one cluster. In general, MLVA types from these clusters were isolated from piglets, sows, and the environment on various sampling events. Piglets carried MLVA types both related and unrelated to their mother sows' MLVA types at farrowing and onwards. In conclusion, molecular typing revealed that within a farm one or a few dominant strain(s) are widespread. Potential MRSA sources for piglets were mother sows, the environment and other piglets.},
  articleno    = {89},
  author       = {Verhegghe, Marijke and Cromb{\'e}, Florence and Pletinckx, Larissa J and Haesebrouck, Freddy and Butaye, Patrick and Herman, Lieve and Heyndrickx, Marc and Rasschaert, Geertrui},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {Genetic diversity,Pigs,Epidemiology,Molecular typing,LA-MRSA,RESISTANT STAPHYLOCOCCUS-AUREUS,FIELD GEL-ELECTROPHORESIS,TANDEM REPEAT ANALYSIS,RAPID IDENTIFICATION,NURSERY PIGLETS,PCR STRATEGY,MULTIPLEX,PIGS,MEC,COLONIZATION},
  language     = {eng},
  pages        = {13},
  title        = {Genetic diversity of livestock-associated MRSA isolates obtained from piglets from farrowing until slaughter age on four farrow-to-finish farms},
  url          = {http://dx.doi.org/10.1186/s13567-014-0089-4},
  volume       = {45},
  year         = {2014},
}

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