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Acinetobacter gandensis sp. nov. isolated from horse and cattle

Annemieke Smet, Piet Cools UGent, Lenka Krizova, Martina Maixnerova, Ondrej Sedo, Freddy Haesebrouck UGent, Marie Kempf, Alexandr Nemec and Mario Vaneechoutte UGent (2014) INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 64(12). p.4007-4015
abstract
We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, % G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4%), Acinetobacter haemolyticus (97.7%), and Acinetobacter schindleri (97.2%). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6%), A. bouvetii NIPH 2281 (88.6%) and A. schindleri CIP 1072871 (87.3%). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C-12:0 3-OH, C-12:0, C-16:0, C-18:1 omega 9c and summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84% with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 % and 20%, respectively. The DNA G C content of strain UG 60467(T) was 39.6 molok. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) (=ANC 4275(T)=LMG 27960(T)=DSM 28097(T)).
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
D BETA-LACTAMASES, CANKER BARK, NEIGHBOR-JOINING METHOD, BAUMANNII, IDENTIFICATION, SPECIMENS, SOIL
journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Int. J. Syst. Evol. Microbiol.
volume
64
issue
12
pages
4007 - 4015
Web of Science type
Article
Web of Science id
000348622000014
JCR category
MICROBIOLOGY
JCR impact factor
2.511 (2014)
JCR rank
60/119 (2014)
JCR quartile
3 (2014)
ISSN
1466-5026
DOI
10.1099/ijs.0.068791-0
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5942097
handle
http://hdl.handle.net/1854/LU-5942097
date created
2015-04-23 14:27:26
date last changed
2016-12-19 15:47:23
@article{5942097,
  abstract     = {We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, \% G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4\%), Acinetobacter haemolyticus (97.7\%), and Acinetobacter schindleri (97.2\%). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6\%), A. bouvetii NIPH 2281 (88.6\%) and A. schindleri CIP 1072871 (87.3\%). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C-12:0 3-OH, C-12:0, C-16:0, C-18:1 omega 9c and summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84\% with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 \% and 20\%, respectively. The DNA G C content of strain UG 60467(T) was 39.6 molok. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) (=ANC 4275(T)=LMG 27960(T)=DSM 28097(T)).},
  author       = {Smet, Annemieke and Cools, Piet and Krizova, Lenka and Maixnerova, Martina and Sedo, Ondrej and Haesebrouck, Freddy and Kempf, Marie and Nemec, Alexandr and Vaneechoutte, Mario},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keyword      = {D BETA-LACTAMASES,CANKER BARK,NEIGHBOR-JOINING METHOD,BAUMANNII,IDENTIFICATION,SPECIMENS,SOIL},
  language     = {eng},
  number       = {12},
  pages        = {4007--4015},
  title        = {Acinetobacter gandensis sp. nov. isolated from horse and cattle},
  url          = {http://dx.doi.org/10.1099/ijs.0.068791-0},
  volume       = {64},
  year         = {2014},
}

Chicago
Smet, Annemieke, Piet Cools, Lenka Krizova, Martina Maixnerova, Ondrej Sedo, Freddy Haesebrouck, Marie Kempf, Alexandr Nemec, and Mario Vaneechoutte. 2014. “Acinetobacter Gandensis Sp. Nov. Isolated from Horse and Cattle.” International Journal of Systematic and Evolutionary Microbiology 64 (12): 4007–4015.
APA
Smet, Annemieke, Cools, P., Krizova, L., Maixnerova, M., Sedo, O., Haesebrouck, F., Kempf, M., et al. (2014). Acinetobacter gandensis sp. nov. isolated from horse and cattle. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(12), 4007–4015.
Vancouver
1.
Smet A, Cools P, Krizova L, Maixnerova M, Sedo O, Haesebrouck F, et al. Acinetobacter gandensis sp. nov. isolated from horse and cattle. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(12):4007–15.
MLA
Smet, Annemieke, Piet Cools, Lenka Krizova, et al. “Acinetobacter Gandensis Sp. Nov. Isolated from Horse and Cattle.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.12 (2014): 4007–4015. Print.