Advanced search
1 file | 174.63 KB Add to list

Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization

Author
Organization
Abstract
Salmonella enterica serovar Enteritidis has developed the potential to contaminate table eggs internally, by colonization of the chicken reproductive tract and internalization in the forming egg. The serotype Enteritidis has developed mechanisms to colonize the chicken oviduct more successfully than other serotypes. Until now, the strategies exploited by Salmonella Enteritidis to do so have remained largely unknown. For that reason, a microarray-based transposon library screen was used to identify genes that are essential for the persistence of Salmonella Enteritidis inside primary chicken oviduct gland cells in vitro and inside the reproductive tract in vivo. A total of 81 genes with a potential role in persistence in both the oviduct cells and the oviduct tissue were identified. Major groups of importance include the Salmonella pathogenicity islands 1 and 2, genes involved in stress responses, cell wall, and lipopolysaccharide structure, and the region-of-difference genomic islands 9, 21, and 40.
Keywords
PATHOGENICITY ISLAND-2, LAYING HENS, SEROTYPE ENTERITIDIS, EGG CONTAMINATION, TYPE-3 SECRETION SYSTEMS, OVIDUCT COLONIZATION, FUNCTIONAL-ANALYSIS, EPITHELIAL-CELLS, GENOME ANALYSIS, TYPHIMURIUM

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 174.63 KB

Citation

Please use this url to cite or link to this publication:

MLA
Raspoet, Ruth et al. “Microarray-based Detection of Salmonella Enterica Serovar Enteritidis Genes Involved in Chicken Reproductive Tract Colonization.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.24 (2014): 7710–7716. Print.
APA
Raspoet, R., Appia-Ayme, C., Shearer, N., Martel, A., Pasmans, F., Haesebrouck, F., Ducatelle, R., et al. (2014). Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(24), 7710–7716.
Chicago author-date
Raspoet, Ruth, C Appia-Ayme, N Shearer, An Martel, Frank Pasmans, Freddy Haesebrouck, Richard Ducatelle, A Thompson, and Filip Van Immerseel. 2014. “Microarray-based Detection of Salmonella Enterica Serovar Enteritidis Genes Involved in Chicken Reproductive Tract Colonization.” Applied and Environmental Microbiology 80 (24): 7710–7716.
Chicago author-date (all authors)
Raspoet, Ruth, C Appia-Ayme, N Shearer, An Martel, Frank Pasmans, Freddy Haesebrouck, Richard Ducatelle, A Thompson, and Filip Van Immerseel. 2014. “Microarray-based Detection of Salmonella Enterica Serovar Enteritidis Genes Involved in Chicken Reproductive Tract Colonization.” Applied and Environmental Microbiology 80 (24): 7710–7716.
Vancouver
1.
Raspoet R, Appia-Ayme C, Shearer N, Martel A, Pasmans F, Haesebrouck F, et al. Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2014;80(24):7710–6.
IEEE
[1]
R. Raspoet et al., “Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization,” APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 80, no. 24, pp. 7710–7716, 2014.
@article{5942077,
  abstract     = {Salmonella enterica serovar Enteritidis has developed the potential to contaminate table eggs internally, by colonization of the chicken reproductive tract and internalization in the forming egg. The serotype Enteritidis has developed mechanisms to colonize the chicken oviduct more successfully than other serotypes. Until now, the strategies exploited by Salmonella Enteritidis to do so have remained largely unknown. For that reason, a microarray-based transposon library screen was used to identify genes that are essential for the persistence of Salmonella Enteritidis inside primary chicken oviduct gland cells in vitro and inside the reproductive tract in vivo. A total of 81 genes with a potential role in persistence in both the oviduct cells and the oviduct tissue were identified. Major groups of importance include the Salmonella pathogenicity islands 1 and 2, genes involved in stress responses, cell wall, and lipopolysaccharide structure, and the region-of-difference genomic islands 9, 21, and 40.},
  author       = {Raspoet, Ruth and Appia-Ayme, C and Shearer, N and Martel, An and Pasmans, Frank and Haesebrouck, Freddy and Ducatelle, Richard and Thompson, A and Van Immerseel, Filip},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keywords     = {PATHOGENICITY ISLAND-2,LAYING HENS,SEROTYPE ENTERITIDIS,EGG CONTAMINATION,TYPE-3 SECRETION SYSTEMS,OVIDUCT COLONIZATION,FUNCTIONAL-ANALYSIS,EPITHELIAL-CELLS,GENOME ANALYSIS,TYPHIMURIUM},
  language     = {eng},
  number       = {24},
  pages        = {7710--7716},
  title        = {Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization},
  url          = {http://dx.doi.org/10.1128/AEM.02867-14},
  volume       = {80},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: