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Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum

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Abstract
In the present study we investigated the taxonomic status of 20 lactic acid bacteria (LAB) originating from packaged meat. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Leuconostoc with Leuconostoc gelidum, Leuconostoc inhae and Leuconostoc gasicomitatum as the closest phylogenetic relatives. The novel strains shared more than 70% DNA-DNA relatedness with type and reference strains of both L. gelidum and L. gasicomitatum. The DNA DNA relatedness values between L. gelidum type and reference strains and L. gasicomitatum type and reference strains were also above 70%, showing that all these strains belonged to the same species. Sequence analyses of concatenated atpA, pheS, and rpoA genes demonstrated that the novel strains as well as type and reference strains of L. gelidum and L. gasicomitatum are phylogenetically closely related, but form three clearly separated subgroups. Numerical analysis of HindIII ribopatterns and phenotypic tests supported this subdivision. Based on the data presented in this study, we propose to reclassify Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov. (type strain, LMG 18811(T)=DSM 15947(T)). The novel strains isolated in the present study represent a novel subspecies, for which the name Leuconostoc gelidum subsp. aenigmaticum subsp. nov. is proposed, with POUF4d(T) (=LMG 27840(T)=DSM 19375(T)) as the type strain. The proposal of these two novel subspecies automatically creates the subspecies Leuconostoc gelidum subsp. gelidum subsp. nov. (type strain, NCFB 2775(T)=DSM 5578(T)). An emended description of Leuconostoc gelidum is also provided.
Keywords
RENATURATION RATES, SEQUENCE-ANALYSIS, DNA HYBRIDIZATION, IDENTIFICATION, KIMCHI, GENES

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MLA
Rahkila, Riitta et al. “Reclassification of Leuconostoc Gasicomitatum as Leuconostoc Gelidum Subsp. Gasicomitatum Comb. Nov., Description of Leuconostoc Gelidum Subsp. Aenigmaticum Subsp. Nov., Designation of Leuconostoc Gelidum Subsp. Gelidum Subsp. Nov. and Emended Description of Leuconostoc Gelidum.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.4 (2014): 1290–1295. Print.
APA
Rahkila, R., De Bruyne, K., Johansson, P., Vandamme, P., & Björkroth, J. (2014). Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(4), 1290–1295.
Chicago author-date
Rahkila, Riitta, Katrien De Bruyne, Per Johansson, Peter Vandamme, and Johanna Björkroth. 2014. “Reclassification of Leuconostoc Gasicomitatum as Leuconostoc Gelidum Subsp. Gasicomitatum Comb. Nov., Description of Leuconostoc Gelidum Subsp. Aenigmaticum Subsp. Nov., Designation of Leuconostoc Gelidum Subsp. Gelidum Subsp. Nov. and Emended Description of Leuconostoc Gelidum.” International Journal of Systematic and Evolutionary Microbiology 64 (4): 1290–1295.
Chicago author-date (all authors)
Rahkila, Riitta, Katrien De Bruyne, Per Johansson, Peter Vandamme, and Johanna Björkroth. 2014. “Reclassification of Leuconostoc Gasicomitatum as Leuconostoc Gelidum Subsp. Gasicomitatum Comb. Nov., Description of Leuconostoc Gelidum Subsp. Aenigmaticum Subsp. Nov., Designation of Leuconostoc Gelidum Subsp. Gelidum Subsp. Nov. and Emended Description of Leuconostoc Gelidum.” International Journal of Systematic and Evolutionary Microbiology 64 (4): 1290–1295.
Vancouver
1.
Rahkila R, De Bruyne K, Johansson P, Vandamme P, Björkroth J. Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(4):1290–5.
IEEE
[1]
R. Rahkila, K. De Bruyne, P. Johansson, P. Vandamme, and J. Björkroth, “Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 4, pp. 1290–1295, 2014.
@article{5941365,
  abstract     = {In the present study we investigated the taxonomic status of 20 lactic acid bacteria (LAB) originating from packaged meat. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Leuconostoc with Leuconostoc gelidum, Leuconostoc inhae and Leuconostoc gasicomitatum as the closest phylogenetic relatives. The novel strains shared more than 70% DNA-DNA relatedness with type and reference strains of both L. gelidum and L. gasicomitatum. The DNA DNA relatedness values between L. gelidum type and reference strains and L. gasicomitatum type and reference strains were also above 70%, showing that all these strains belonged to the same species. Sequence analyses of concatenated atpA, pheS, and rpoA genes demonstrated that the novel strains as well as type and reference strains of L. gelidum and L. gasicomitatum are phylogenetically closely related, but form three clearly separated subgroups. Numerical analysis of HindIII ribopatterns and phenotypic tests supported this subdivision. Based on the data presented in this study, we propose to reclassify Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov. (type strain, LMG 18811(T)=DSM 15947(T)). The novel strains isolated in the present study represent a novel subspecies, for which the name Leuconostoc gelidum subsp. aenigmaticum subsp. nov. is proposed, with POUF4d(T) (=LMG 27840(T)=DSM 19375(T)) as the type strain. The proposal of these two novel subspecies automatically creates the subspecies Leuconostoc gelidum subsp. gelidum subsp. nov. (type strain, NCFB 2775(T)=DSM 5578(T)). An emended description of Leuconostoc gelidum is also provided.},
  author       = {Rahkila, Riitta and De Bruyne, Katrien and Johansson, Per and Vandamme, Peter and Björkroth, Johanna},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {RENATURATION RATES,SEQUENCE-ANALYSIS,DNA HYBRIDIZATION,IDENTIFICATION,KIMCHI,GENES},
  language     = {eng},
  number       = {4},
  pages        = {1290--1295},
  title        = {Reclassification of Leuconostoc gasicomitatum as Leuconostoc gelidum subsp. gasicomitatum comb. nov., description of Leuconostoc gelidum subsp. aenigmaticum subsp. nov., designation of Leuconostoc gelidum subsp. gelidum subsp. nov. and emended description of Leuconostoc gelidum},
  url          = {http://dx.doi.org/10.1099/ijs.0.058263-0},
  volume       = {64},
  year         = {2014},
}

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