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Carnosine loading and washout in human skeletal muscles

Audrey Baguet UGent, Harmen Reyngoudt UGent, Andries Pottier, Inge Everaert UGent, Stefanie Callens, Eric Achten UGent and Wim Derave UGent (2009) JOURNAL OF APPLIED PHYSIOLOGY. 106(3). p.837-842
abstract
Carnosine (beta-alanyl-L-histidine) is present in high concentrations in human skeletal muscles. The oral ingestion of beta-alanine, the rate-limiting precursor in carnosine synthesis, has been shown to elevate the muscle carnosine content both in trained and untrained humans. Little human data exist about the dynamics of the muscle carnosine content, its metabolic regulation, and its dependence on muscle fiber type. The present study aimed to investigate in three skeletal muscle types the supplementation-induced amplitude of carnosine synthesis and its subsequent elimination on cessation of supplementation (washout). Fifteen untrained males participated in a placebo-controlled double-blind study. They were supplemented for 5-6 wk with either 4.8 g/day beta-alanine or placebo. Muscle carnosine was quantified in soleus, tibialis anterior, and medial head of the gastrocnemius by proton magnetic resonance spectroscopy (MRS), before and after supplementation and 3 and 9 wk into washout. The beta-alanine supplementation significantly increased the carnosine content in soleus by 39%, in tibialis by 27%, and in gastrocnemius by 23% and declined post-supplementation at a rate of 2-4%/ wk. Average muscle carnosine remained increased compared with baseline at 3 wk of washout ( only one-third of the supplementation-induced increase had disappeared) and returned to baseline values within 9 wk at group level. Following subdivision into high responders (+55%) and low responders (+15%), washout period was 15 and 6 wk, respectively. In the placebo group, carnosine remained relatively constant with variation coefficients of 9-15% over a 3-mo period. It can be concluded that carnosine is a stable compound in human skeletal muscle, confirming the absence of carnosinase in myocytes. The present study shows that washout periods for crossover designs in supplementation studies for muscle metabolites may sometimes require months rather than weeks.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ergogenic supplements, skeletal muscle metabolism, washout, carnosinase, magnetic resonance spectroscopy
journal title
JOURNAL OF APPLIED PHYSIOLOGY
J. Appl. Physiol.
volume
106
issue
3
pages
837 - 842
publisher
AMER PHYSIOLOGICAL SOC
place of publication
BETHESDA
Web of Science type
article
Web of Science id
000263719700013
JCR category
SPORT SCIENCES
JCR impact factor
3.732 (2009)
JCR rank
1/72 (2009)
JCR quartile
1 (2009)
ISSN
8750-7587
DOI
10.1152/japplphysiol.91357.2008
language
English
UGent publication?
yes
classification
A1
copyright statement
I don't know the status of the copyright for this publication
id
593316
handle
http://hdl.handle.net/1854/LU-593316
date created
2009-04-10 15:12:46
date last changed
2015-06-17 11:10:03
@article{593316,
  abstract     = {Carnosine (beta-alanyl-L-histidine) is present in high concentrations in human skeletal muscles. The oral ingestion of beta-alanine, the rate-limiting precursor in carnosine synthesis, has been shown to elevate the muscle carnosine content both in trained and untrained humans. Little human data exist about the dynamics of the muscle carnosine content, its metabolic regulation, and its dependence on muscle fiber type. The present study aimed to investigate in three skeletal muscle types the supplementation-induced amplitude of carnosine synthesis and its subsequent elimination on cessation of supplementation (washout). Fifteen untrained males participated in a placebo-controlled double-blind study. They were supplemented for 5-6 wk with either 4.8 g/day beta-alanine or placebo. Muscle carnosine was quantified in soleus, tibialis anterior, and medial head of the gastrocnemius by proton magnetic resonance spectroscopy (MRS), before and after supplementation and 3 and 9 wk into washout. The beta-alanine supplementation significantly increased the carnosine content in soleus by 39\%, in tibialis by 27\%, and in gastrocnemius by 23\% and declined post-supplementation at a rate of 2-4\%/ wk. Average muscle carnosine remained increased compared with baseline at 3 wk of washout ( only one-third of the supplementation-induced increase had disappeared) and returned to baseline values within 9 wk at group level. Following subdivision into high responders (+55\%) and low responders (+15\%), washout period was 15 and 6 wk, respectively. In the placebo group, carnosine remained relatively constant with variation coefficients of 9-15\% over a 3-mo period. It can be concluded that carnosine is a stable compound in human skeletal muscle, confirming the absence of carnosinase in myocytes. The present study shows that washout periods for crossover designs in supplementation studies for muscle metabolites may sometimes require months rather than weeks.},
  author       = {Baguet, Audrey and Reyngoudt, Harmen and Pottier, Andries and Everaert, Inge and Callens, Stefanie and Achten, Eric and Derave, Wim},
  issn         = {8750-7587},
  journal      = {JOURNAL OF APPLIED PHYSIOLOGY},
  keyword      = {ergogenic supplements,skeletal muscle metabolism,washout,carnosinase,magnetic resonance spectroscopy},
  language     = {eng},
  number       = {3},
  pages        = {837--842},
  publisher    = {AMER PHYSIOLOGICAL SOC},
  title        = {Carnosine loading and washout in human skeletal muscles},
  url          = {http://dx.doi.org/10.1152/japplphysiol.91357.2008},
  volume       = {106},
  year         = {2009},
}

Chicago
Baguet, Audrey, Harmen Reyngoudt, Andries Pottier, Inge Everaert, Stefanie Callens, Eric Achten, and Wim Derave. 2009. “Carnosine Loading and Washout in Human Skeletal Muscles.” Journal of Applied Physiology 106 (3): 837–842.
APA
Baguet, A., Reyngoudt, H., Pottier, A., Everaert, I., Callens, S., Achten, E., & Derave, W. (2009). Carnosine loading and washout in human skeletal muscles. JOURNAL OF APPLIED PHYSIOLOGY, 106(3), 837–842.
Vancouver
1.
Baguet A, Reyngoudt H, Pottier A, Everaert I, Callens S, Achten E, et al. Carnosine loading and washout in human skeletal muscles. JOURNAL OF APPLIED PHYSIOLOGY. BETHESDA: AMER PHYSIOLOGICAL SOC; 2009;106(3):837–42.
MLA
Baguet, Audrey, Harmen Reyngoudt, Andries Pottier, et al. “Carnosine Loading and Washout in Human Skeletal Muscles.” JOURNAL OF APPLIED PHYSIOLOGY 106.3 (2009): 837–842. Print.