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Deficiency of the Arabidopsis helicase RTEL1 triggers a SOG1-dependent replication checkpoint in response to DNA cross-links

Zhubing Hu (UGent) , Toon Cools (UGent) , Pooneh Kalhorzadeh (UGent) , Jefri Heyman (UGent) and Lieven De Veylder (UGent)
(2015) PLANT CELL. 27(1). p.149-161
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Abstract
To maintain genome integrity, DNA replication is executed and regulated by a complex molecular network of numerous proteins, including helicases and cell cycle checkpoint regulators. Through a systematic screening for putative replication mutants, we identified an Arabidopsis thaliana homolog of human Regulator of Telomere Length 1 (RTEL1), which functions in DNA replication, DNA repair, and recombination. RTEL1 deficiency retards plant growth, a phenotype including a prolonged S-phase duration and decreased cell proliferation. Genetic analysis revealed that rtel1 mutant plants show activated cell cycle checkpoints, specific sensitivity to DNA cross-linking agents, and increased homologous recombination, but a lack of progressive shortening of telomeres, indicating that RTEL1 functions have only been partially conserved between mammals and plants. Surprisingly, RTEL1 deficiency induces tolerance to the deoxynucleotide-depleting drug hydroxyurea, which could be mimicked by DNA cross-linking agents. This resistance does not rely on the essential replication checkpoint regulator WEE1 but could be blocked by a mutation in the SOG1 transcription factor. Taken together, our data indicate that RTEL1 is required for DNA replication and that its deficiency activates a SOG1-dependent replication checkpoint.
Keywords
KINASE, GENE, DAMAGE, PLANTS, REPAIR, THALIANA, GENOME INSTABILITY, HOMOLOGOUS RECOMBINATION, TELOMERE DYSFUNCTION, INTEGRITY

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Chicago
Hu, Zhubing, Toon Cools, Pooneh Kalhorzadeh, Jefri Heyman, and Lieven De Veylder. 2015. “Deficiency of the Arabidopsis Helicase RTEL1 Triggers a SOG1-dependent Replication Checkpoint in Response to DNA Cross-links.” Plant Cell 27 (1): 149–161.
APA
Hu, Z., Cools, T., Kalhorzadeh, P., Heyman, J., & De Veylder, L. (2015). Deficiency of the Arabidopsis helicase RTEL1 triggers a SOG1-dependent replication checkpoint in response to DNA cross-links. PLANT CELL, 27(1), 149–161.
Vancouver
1.
Hu Z, Cools T, Kalhorzadeh P, Heyman J, De Veylder L. Deficiency of the Arabidopsis helicase RTEL1 triggers a SOG1-dependent replication checkpoint in response to DNA cross-links. PLANT CELL. 2015;27(1):149–61.
MLA
Hu, Zhubing, Toon Cools, Pooneh Kalhorzadeh, et al. “Deficiency of the Arabidopsis Helicase RTEL1 Triggers a SOG1-dependent Replication Checkpoint in Response to DNA Cross-links.” PLANT CELL 27.1 (2015): 149–161. Print.
@article{5930802,
  abstract     = {To maintain genome integrity, DNA replication is executed and regulated by a complex molecular network of numerous proteins, including helicases and cell cycle checkpoint regulators. Through a systematic screening for putative replication mutants, we identified an Arabidopsis thaliana homolog of human Regulator of Telomere Length 1 (RTEL1), which functions in DNA replication, DNA repair, and recombination. RTEL1 deficiency retards plant growth, a phenotype including a prolonged S-phase duration and decreased cell proliferation. Genetic analysis revealed that rtel1 mutant plants show activated cell cycle checkpoints, specific sensitivity to DNA cross-linking agents, and increased homologous recombination, but a lack of progressive shortening of telomeres, indicating that RTEL1 functions have only been partially conserved between mammals and plants. Surprisingly, RTEL1 deficiency induces tolerance to the deoxynucleotide-depleting drug hydroxyurea, which could be mimicked by DNA cross-linking agents. This resistance does not rely on the essential replication checkpoint regulator WEE1 but could be blocked by a mutation in the SOG1 transcription factor. Taken together, our data indicate that RTEL1 is required for DNA replication and that its deficiency activates a SOG1-dependent replication checkpoint.},
  author       = {Hu, Zhubing and Cools, Toon and Kalhorzadeh, Pooneh and Heyman, Jefri and De Veylder, Lieven},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  language     = {eng},
  number       = {1},
  pages        = {149--161},
  title        = {Deficiency of the Arabidopsis helicase RTEL1 triggers a SOG1-dependent replication checkpoint in response to DNA cross-links},
  url          = {http://dx.doi.org/10.1105/tpc.114.134312},
  volume       = {27},
  year         = {2015},
}

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