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Structural and functional investigation of a secreted chorismate mutase from the plant-parasitic nematode Heterodera schachtii in the context of related enzymes from diverse origin

Bartel Vanholme UGent, Peter Kast, Annelies Haegeman UGent, Joachim Jacob UGent, Wim Grunewald UGent and Godelieve Gheysen UGent (2009) Molecular Plant Pathology. 10(2). p.189-200
abstract
In this article, we present the cloning of Hscm1, a gene for chorismate mutase (CM) from the beet cyst nematode Heterodera schachtii. CM is a key branch-point enzyme of the shikimate pathway, and secondary metabolites that arise from this pathway control developmental programmes and defence responses of the plant. By manipulating the plant's endogenous shikimate pathway, the nematode can influence the plant physiology for its own benefit. Hscm1 is a member of the CM gene family and is expressed during the pre-parasitic and parasitic stages of the nematode's life cycle. In situ mRNA hybridization reveals an expression pattern specific to the subventral and dorsal pharyngeal glands. The predicted protein has a signal peptide for secretion in addition to two domains. The N-terminal domain of the mature protein, which is only found in cyst nematodes, contains six conserved cysteine residues, which may reflect the importance of disulphide bond formation for protein stabilization. The C-terminal domain holds a single catalytic site and has similarity to secreted CMs of pathogenic bacteria, classifying HsCM1 as an AroQ(gamma) enzyme. The presumed catalytic residues are discussed in detail, and genetic complementation experiments indicate that the C-terminal domain is essential for enzyme activity. Finally, we show how the modular design of the protein is mirrored in the genomic sequence by the intron/exon organization, suggesting exon shuffling as a mechanism for the evolutionary assembly of this protein.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
Molecular Plant Pathology
Mol. Plant Pathol.
volume
10
issue
2
pages
189 - 200
Web of Science type
Article
Web of Science id
000263256800004
JCR category
PLANT SCIENCES
JCR impact factor
3.455 (2009)
JCR rank
19/172 (2009)
JCR quartile
1 (2009)
ISSN
1464-6722
DOI
10.1111/j.1364-3703.2008.00521.x
language
English
UGent publication?
yes
classification
A1
id
593044
handle
http://hdl.handle.net/1854/LU-593044
date created
2009-04-09 18:10:53
date last changed
2009-04-10 14:37:22
@article{593044,
  abstract     = {In this article, we present the cloning of Hscm1, a gene for chorismate mutase (CM) from the beet cyst nematode Heterodera schachtii. CM is a key branch-point enzyme of the shikimate pathway, and secondary metabolites that arise from this pathway control developmental programmes and defence responses of the plant. By manipulating the plant's endogenous shikimate pathway, the nematode can influence the plant physiology for its own benefit. Hscm1 is a member of the CM gene family and is expressed during the pre-parasitic and parasitic stages of the nematode's life cycle. In situ mRNA hybridization reveals an expression pattern specific to the subventral and dorsal pharyngeal glands. The predicted protein has a signal peptide for secretion in addition to two domains. The N-terminal domain of the mature protein, which is only found in cyst nematodes, contains six conserved cysteine residues, which may reflect the importance of disulphide bond formation for protein stabilization. The C-terminal domain holds a single catalytic site and has similarity to secreted CMs of pathogenic bacteria, classifying HsCM1 as an AroQ(gamma) enzyme. The presumed catalytic residues are discussed in detail, and genetic complementation experiments indicate that the C-terminal domain is essential for enzyme activity. Finally, we show how the modular design of the protein is mirrored in the genomic sequence by the intron/exon organization, suggesting exon shuffling as a mechanism for the evolutionary assembly of this protein.},
  author       = {Vanholme, Bartel and Kast, Peter and Haegeman, Annelies and Jacob, Joachim and Grunewald, Wim and Gheysen, Godelieve},
  issn         = {1464-6722},
  journal      = {Molecular Plant Pathology},
  language     = {eng},
  number       = {2},
  pages        = {189--200},
  title        = {Structural and functional investigation of a secreted chorismate mutase from the plant-parasitic nematode Heterodera schachtii in the context of related enzymes from diverse origin},
  url          = {http://dx.doi.org/10.1111/j.1364-3703.2008.00521.x},
  volume       = {10},
  year         = {2009},
}

Chicago
Vanholme, Bartel, Peter Kast, Annelies Haegeman, Joachim Jacob, Wim Grunewald, and Godelieve Gheysen. 2009. “Structural and Functional Investigation of a Secreted Chorismate Mutase from the Plant-parasitic Nematode Heterodera Schachtii in the Context of Related Enzymes from Diverse Origin.” Molecular Plant Pathology 10 (2): 189–200.
APA
Vanholme, B., Kast, P., Haegeman, A., Jacob, J., Grunewald, W., & Gheysen, G. (2009). Structural and functional investigation of a secreted chorismate mutase from the plant-parasitic nematode Heterodera schachtii in the context of related enzymes from diverse origin. Molecular Plant Pathology, 10(2), 189–200.
Vancouver
1.
Vanholme B, Kast P, Haegeman A, Jacob J, Grunewald W, Gheysen G. Structural and functional investigation of a secreted chorismate mutase from the plant-parasitic nematode Heterodera schachtii in the context of related enzymes from diverse origin. Molecular Plant Pathology. 2009;10(2):189–200.
MLA
Vanholme, Bartel, Peter Kast, Annelies Haegeman, et al. “Structural and Functional Investigation of a Secreted Chorismate Mutase from the Plant-parasitic Nematode Heterodera Schachtii in the Context of Related Enzymes from Diverse Origin.” Molecular Plant Pathology 10.2 (2009): 189–200. Print.