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Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking

Hirokazu Tanaka (UGent) , Saeko Kitakura (UGent) , Riet De Rycke (UGent) , Ruth De Groodt and Jiri Friml (UGent)
(2009) Current Biology. 19(5). p.391-397
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Abstract
Endocytic vesicle trafficking is crucial for regulating activity and localization of plasma membrane components, but the process Is still poorly genetically defined In plants. Membrane proteins of the PIN-FORMED (PIN) family exhibit polar localization In plant cells and facilitate cellular efflux of the plant hormone auxin, thereby regulating multiple developmental processes [1, 2]. PIN proteins undergo constitutive endocytosis and GNOM ARF GEF-dependent recycling [3-5], and their localization Is under extensive regulation by developmental and environmental cues [6-9]. We designed a fluorescence Imaging-based screen to Identify Arabidopsis thaliana mutants defective in internalization of proteins including PINs from the plasma membrane. We Identified three mutant loci, BFA-visualized endocytic trafficking defective1 (ben1) through ben3that do not efficiently accumulate PIN1-GFP In Intracellular compartments after Inhibition of recycling and secretion by fungal toxin brefeldin A (BFA). Fine mapping revealed that BEN1 encodes an ARF GEF vesicle trafficking regulator from the functionally uncharacterized BIG class. ben 1 mutant has been previously Implicated in pathogen response [10] and shows cell polarity, BFA sensitivity, and growth defects. BEN1 is Involved in endocytosis of plasma membrane proteins and localizes to early endocytic compartments distinct from GNOM-positive endosomes. Our results Identify BEN1 as the ARF GEF mediating early endosomal traffic.

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MLA
Tanaka, Hirokazu et al. “Fluorescence Imaging-based Screen Identifies ARF GEF Component of Early Endosomal Trafficking.” Current Biology 19.5 (2009): 391–397. Print.
APA
Tanaka, H., Kitakura, S., De Rycke, R., De Groodt, R., & Friml, J. (2009). Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking. Current Biology, 19(5), 391–397.
Chicago author-date
Tanaka, Hirokazu, Saeko Kitakura, Riet De Rycke, Ruth De Groodt, and Jiri Friml. 2009. “Fluorescence Imaging-based Screen Identifies ARF GEF Component of Early Endosomal Trafficking.” Current Biology 19 (5): 391–397.
Chicago author-date (all authors)
Tanaka, Hirokazu, Saeko Kitakura, Riet De Rycke, Ruth De Groodt, and Jiri Friml. 2009. “Fluorescence Imaging-based Screen Identifies ARF GEF Component of Early Endosomal Trafficking.” Current Biology 19 (5): 391–397.
Vancouver
1.
Tanaka H, Kitakura S, De Rycke R, De Groodt R, Friml J. Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking. Current Biology. 2009;19(5):391–7.
IEEE
[1]
H. Tanaka, S. Kitakura, R. De Rycke, R. De Groodt, and J. Friml, “Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking,” Current Biology, vol. 19, no. 5, pp. 391–397, 2009.
@article{593010,
  abstract     = {{Endocytic vesicle trafficking is crucial for regulating activity and localization of plasma membrane components, but the process Is still poorly genetically defined In plants. Membrane proteins of the PIN-FORMED (PIN) family exhibit polar localization In plant cells and facilitate cellular efflux of the plant hormone auxin, thereby regulating multiple developmental processes [1, 2]. PIN proteins undergo constitutive endocytosis and GNOM ARF GEF-dependent recycling [3-5], and their localization Is under extensive regulation by developmental and environmental cues [6-9]. We designed a fluorescence Imaging-based screen to Identify Arabidopsis thaliana mutants defective in internalization of proteins including PINs from the plasma membrane. We Identified three mutant loci, BFA-visualized endocytic trafficking defective1 (ben1) through ben3that do not efficiently accumulate PIN1-GFP In Intracellular compartments after Inhibition of recycling and secretion by fungal toxin brefeldin A (BFA). Fine mapping revealed that BEN1 encodes an ARF GEF vesicle trafficking regulator from the functionally uncharacterized BIG class. ben 1 mutant has been previously Implicated in pathogen response [10] and shows cell polarity, BFA sensitivity, and growth defects. BEN1 is Involved in endocytosis of plasma membrane proteins and localizes to early endocytic compartments distinct from GNOM-positive endosomes. Our results Identify BEN1 as the ARF GEF mediating early endosomal traffic.}},
  author       = {{Tanaka, Hirokazu and Kitakura, Saeko and De Rycke, Riet and De Groodt, Ruth and Friml, Jiri}},
  issn         = {{0960-9822}},
  journal      = {{Current Biology}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{391--397}},
  title        = {{Fluorescence imaging-based screen identifies ARF GEF component of early endosomal trafficking}},
  url          = {{http://dx.doi.org/10.1016/j.cub.2009.01.057}},
  volume       = {{19}},
  year         = {{2009}},
}

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