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Imaging regulatory T cell dynamics and CTLA4-mediated suppression of T cell priming

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Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
Abstract
Foxp3(+) regulatory T cells (Tregs) maintain immune homoeostasis through mechanisms that remain incompletely defined. Here by two-photon (2P) imaging, we examine the cellular dynamics of endogenous Tregs. Tregs are identified as two non-overlapping populations in the T-zone and follicular regions of the lymph node (LN). In the T-zone, Tregs migrate more rapidly than conventional T cells (Tconv), extend longer processes and interact with resident dendritic cells (DC) and Tconv. Tregs intercept immigrant DCs and interact with antigen-induced DC: Tconv clusters, while continuing to form contacts with activated Tconv. During antigen-specific responses, blocking CTLA4-B7 interactions reduces Treg-Tconv interaction times, increases the volume of DC: Tconv clusters and enhances subsequent Tconv proliferation in vivo. Our results demonstrate a role for altered cellular choreography of Tregs through CTLA4-based interactions to limit T-cell priming.
Keywords
LYMPH-NODE, IMMUNE-RESPONSE, IN-VIVO, DENDRITIC CELLS, TCR STOP-SIGNAL, COSTIMULATORY MOLECULES, CTLA-4, ANTIGEN, ACTIVATION, TOLERANCE

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Citation

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Chicago
Matheu, Melanie, Shivashankar Othy, Milton L Greenberg, Tobias X Dong, Martijn Schuijs, Kim Deswarte, Hamida Hammad, Bart Lambrecht, Ian Parker, and Michael D Cahalan. 2015. “Imaging Regulatory T Cell Dynamics and CTLA4-mediated Suppression of T Cell Priming.” Nature Communications 6.
APA
Matheu, M., Othy, S., Greenberg, M. L., Dong, T. X., Schuijs, M., Deswarte, K., Hammad, H., et al. (2015). Imaging regulatory T cell dynamics and CTLA4-mediated suppression of T cell priming. NATURE COMMUNICATIONS, 6.
Vancouver
1.
Matheu M, Othy S, Greenberg ML, Dong TX, Schuijs M, Deswarte K, et al. Imaging regulatory T cell dynamics and CTLA4-mediated suppression of T cell priming. NATURE COMMUNICATIONS. 2015;6.
MLA
Matheu, Melanie et al. “Imaging Regulatory T Cell Dynamics and CTLA4-mediated Suppression of T Cell Priming.” NATURE COMMUNICATIONS 6 (2015): n. pag. Print.
@article{5929967,
  abstract     = {Foxp3(+) regulatory T cells (Tregs) maintain immune homoeostasis through mechanisms that remain incompletely defined. Here by two-photon (2P) imaging, we examine the cellular dynamics of endogenous Tregs. Tregs are identified as two non-overlapping populations in the T-zone and follicular regions of the lymph node (LN). In the T-zone, Tregs migrate more rapidly than conventional T cells (Tconv), extend longer processes and interact with resident dendritic cells (DC) and Tconv. Tregs intercept immigrant DCs and interact with antigen-induced DC: Tconv clusters, while continuing to form contacts with activated Tconv. During antigen-specific responses, blocking CTLA4-B7 interactions reduces Treg-Tconv interaction times, increases the volume of DC: Tconv clusters and enhances subsequent Tconv proliferation in vivo. Our results demonstrate a role for altered cellular choreography of Tregs through CTLA4-based interactions to limit T-cell priming.},
  articleno    = {6219},
  author       = {Matheu, Melanie and Othy, Shivashankar and Greenberg, Milton L and Dong, Tobias X and Schuijs, Martijn and Deswarte, Kim and Hammad, Hamida and Lambrecht, Bart and Parker, Ian and Cahalan, Michael D},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {LYMPH-NODE,IMMUNE-RESPONSE,IN-VIVO,DENDRITIC CELLS,TCR STOP-SIGNAL,COSTIMULATORY MOLECULES,CTLA-4,ANTIGEN,ACTIVATION,TOLERANCE},
  language     = {eng},
  pages        = {11},
  title        = {Imaging regulatory T cell dynamics and CTLA4-mediated suppression of T cell priming},
  url          = {http://dx.doi.org/10.1038/ncomms7219},
  volume       = {6},
  year         = {2015},
}

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