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CCR2⁺CD103⁻ intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells

(2015) MUCOSAL IMMUNOLOGY. 8(2). p.327-339
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Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
Abstract
The identification of intestinal macrophages (m phi s) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and m phi s based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C hi monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by Tcells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.
Keywords
LAMINA-PROPRIA, CHEMOKINE RECEPTOR, LY6C(HI) MONOCYTES, MICE LACKING, MACROPHAGES, DIFFERENTIATION, HOMEOSTASIS, EXPRESSION, RESPONSES, MUCOSAL

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MLA
Scott, Charlotte et al. “CCR2+CD103 Intestinal Dendritic Cells Develop from DC-committed Precursors and Induce Interleukin-17 Production by T Cells.” MUCOSAL IMMUNOLOGY 8.2 (2015): 327–339. Print.
APA
Scott, C., Bain, C., Wright, P., Sichien, D., Kotarsky, K., Persson, E., Luda, K., et al. (2015). CCR2+CD103 intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells. MUCOSAL IMMUNOLOGY, 8(2), 327–339.
Chicago author-date
Scott, Charlotte, CC Bain, PB Wright, Dorine Sichien, K Kotarsky, Emma Persson, K Luda, et al. 2015. “CCR2+CD103 Intestinal Dendritic Cells Develop from DC-committed Precursors and Induce Interleukin-17 Production by T Cells.” Mucosal Immunology 8 (2): 327–339.
Chicago author-date (all authors)
Scott, Charlotte, CC Bain, PB Wright, Dorine Sichien, K Kotarsky, Emma Persson, K Luda, Martin Guilliams, Bart Lambrecht, WW Agace, SWF Milling, and AM Mowat. 2015. “CCR2+CD103 Intestinal Dendritic Cells Develop from DC-committed Precursors and Induce Interleukin-17 Production by T Cells.” Mucosal Immunology 8 (2): 327–339.
Vancouver
1.
Scott C, Bain C, Wright P, Sichien D, Kotarsky K, Persson E, et al. CCR2+CD103 intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells. MUCOSAL IMMUNOLOGY. 2015;8(2):327–39.
IEEE
[1]
C. Scott et al., “CCR2+CD103 intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells,” MUCOSAL IMMUNOLOGY, vol. 8, no. 2, pp. 327–339, 2015.
@article{5929902,
  abstract     = {The identification of intestinal macrophages (m phi s) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and m phi s based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C hi monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by Tcells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.},
  author       = {Scott, Charlotte and Bain, CC and Wright, PB and Sichien, Dorine and Kotarsky, K and Persson, Emma and Luda, K and Guilliams, Martin and Lambrecht, Bart and Agace, WW and Milling, SWF and Mowat, AM},
  issn         = {1933-0219},
  journal      = {MUCOSAL IMMUNOLOGY},
  keywords     = {LAMINA-PROPRIA,CHEMOKINE RECEPTOR,LY6C(HI) MONOCYTES,MICE LACKING,MACROPHAGES,DIFFERENTIATION,HOMEOSTASIS,EXPRESSION,RESPONSES,MUCOSAL},
  language     = {eng},
  number       = {2},
  pages        = {327--339},
  title        = {CCR2⁺CD103⁻ intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells},
  url          = {http://dx.doi.org/10.1038/mi.2014.70},
  volume       = {8},
  year         = {2015},
}

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