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Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications

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Center for nano- and biophotonics (NB-Photonics)
Abstract
Porous biodegradable scaffolds represent promising candidates for tissue-engineering applications because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold designed with the aim of inducing and supporting enzyme-mediated formation of apatite minerals in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase (ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical findings revealed homogeneous mineralization, with the phase structure of the formed minerals resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and colonization. Copyright © 2015 John Wiley & Sons, Ltd.
Keywords
chitosan, enzyme, alkaline phosphatase, biomineralization, scaffolds, bone, osteogenic, tissue engineering, CALCIUM-PHOSPHATE MINERALIZATION, ALKALINE-PHOSPHATASE, BONE SUBSTITUTES, CEMENT COMPOSITES, APATITE FORMATION, METAL-IONS, BIODEGRADATION, DEACETYLATION, DEGRADATION, HYDROGELS

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Citation

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Chicago
Dash, Mamoni, Sangram Keshari Samal, Timothy Douglas, David Schaubroeck, Sander C Leeuwenburgh, Pascal Van Der Voort, Heidi Declercq, and Peter Dubruel. 2017. “Enzymatically Biomineralized Chitosan Scaffolds for Tissue-engineering Applications.” Journal of Tissue Engineering and Regenerative Medicine 11 (5): 1500–1513.
APA
Dash, M., Samal, S. K., Douglas, T., Schaubroeck, D., Leeuwenburgh, S. C., Van Der Voort, P., Declercq, H., et al. (2017). Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 11(5), 1500–1513.
Vancouver
1.
Dash M, Samal SK, Douglas T, Schaubroeck D, Leeuwenburgh SC, Van Der Voort P, et al. Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE. 2017;11(5):1500–13.
MLA
Dash, Mamoni, Sangram Keshari Samal, Timothy Douglas, et al. “Enzymatically Biomineralized Chitosan Scaffolds for Tissue-engineering Applications.” JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 11.5 (2017): 1500–1513. Print.
@article{5927969,
  abstract     = {Porous biodegradable scaffolds represent promising candidates for tissue-engineering applications
because of their capability to be preseeded with cells. We report an uncrosslinked chitosan scaffold
designed with the aim of inducing and supporting enzyme-mediated formation of apatite minerals
in the absence of osteogenic growth factors. To realize this, natural enzyme alkaline phosphatase
(ALP) was incorporated into uncrosslinked chitosan scaffolds. The uncrosslinked chitosan makes
available amine and alcohol functionalities to enhance the biomineralization process. The physicochemical
findings revealed homogeneous mineralization, with the phase structure of the formed minerals
resembling that of apatite at low mineral concentrations, and similar to dicalcium phosphate
dihydrate (DCPD) with increasing ALP content. The MC3T3 cell activity clearly showed that the mineralization
of the chitosan scaffolds was effective in improving cellular adhesion, proliferation and
colonization. Copyright {\textcopyright} 2015 John Wiley \& Sons, Ltd.},
  author       = {Dash, Mamoni and Samal, Sangram Keshari and Douglas, Timothy and Schaubroeck, David and Leeuwenburgh, Sander C and Van Der Voort, Pascal and Declercq, Heidi and Dubruel, Peter},
  issn         = {1932-6254},
  journal      = {JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE},
  keyword      = {chitosan,enzyme,alkaline phosphatase,biomineralization,scaffolds,bone,osteogenic,tissue engineering,CALCIUM-PHOSPHATE MINERALIZATION,ALKALINE-PHOSPHATASE,BONE SUBSTITUTES,CEMENT COMPOSITES,APATITE FORMATION,METAL-IONS,BIODEGRADATION,DEACETYLATION,DEGRADATION,HYDROGELS},
  language     = {eng},
  number       = {5},
  pages        = {1500--1513},
  title        = {Enzymatically biomineralized chitosan scaffolds for tissue-engineering applications},
  url          = {http://dx.doi.org/10.1002/term.2048},
  volume       = {11},
  year         = {2017},
}

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