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Change of a conserved amino acid in the MYC2 and MYC3 transcription factors leads to release of JAZ repression and increased activity

Jonas Goossens (UGent) , Gwen Swinnen (UGent) , Robin Vanden Bossche (UGent) , Laurens Pauwels (UGent) and Alain Goossens (UGent)
(2015) NEW PHYTOLOGIST. 206(4). p.1229-1237
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Abstract
The bHLH transcription factor MYC2, together with its paralogues MYC3 and MYC4, is a master regulator of the response to the jasmonate (JA) hormone in Arabidopsis (Arabidopsis thaliana). In the absence of JA, JASMONATE ZIM (JAZ) proteins interact with the MYC proteins to block their activity. Understanding of the mechanism and specificity of this interaction is key to unravel JA signalling. We generated mutant MYC proteins and assessed their activity and the specificity of their interaction with the 12 Arabidopsis JAZ proteins. We show that the D94N mutation present in the atr2D allele of MYC3 abolishes the interaction between MYC3 and most JAZ proteins. The same effect is observed when the corresponding conserved Asp (D105) was mutated in MYC2. Accordingly, MYC2(D105N) activated target genes in the presence of JAZ proteins, in contrast to wild-type MYC2. JAZ1 and JAZ10 were the only JAZ proteins still showing interaction with the mutant MYC proteins, due to a second MYC interaction domain, besides the classical Jas domain. Our results visualize the divergence among JAZ proteins in their interaction with MYC proteins. Ultimately, the transferability of the Asp-to-Asn amino acid change might facilitate the design of hyperactive transcription factors for plant engineering.
Keywords
Arabidopsis, abscisic acid (ABA), bHLH transcription factor, jasmonate (JA), JAZ, MYC2, transactivation, JASMONATE RESPONSES, GLUCOSINOLATE BIOSYNTHESIS, ARABIDOPSIS-THALIANA, GENE-EXPRESSION, SPLICE VARIANT, PROTEINS, DEFENSE, TARGETS, GROWTH, MOTIF

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Chicago
Goossens, Jonas, Gwen Swinnen, Robin Vanden Bossche, Laurens Pauwels, and Alain Goossens. 2015. “Change of a Conserved Amino Acid in the MYC2 and MYC3 Transcription Factors Leads to Release of JAZ Repression and Increased Activity.” New Phytologist 206 (4): 1229–1237.
APA
Goossens, Jonas, Swinnen, G., Vanden Bossche, R., Pauwels, L., & Goossens, A. (2015). Change of a conserved amino acid in the MYC2 and MYC3 transcription factors leads to release of JAZ repression and increased activity. NEW PHYTOLOGIST, 206(4), 1229–1237.
Vancouver
1.
Goossens J, Swinnen G, Vanden Bossche R, Pauwels L, Goossens A. Change of a conserved amino acid in the MYC2 and MYC3 transcription factors leads to release of JAZ repression and increased activity. NEW PHYTOLOGIST. 2015;206(4):1229–37.
MLA
Goossens, Jonas et al. “Change of a Conserved Amino Acid in the MYC2 and MYC3 Transcription Factors Leads to Release of JAZ Repression and Increased Activity.” NEW PHYTOLOGIST 206.4 (2015): 1229–1237. Print.
@article{5927666,
  abstract     = {The bHLH transcription factor MYC2, together with its paralogues MYC3 and MYC4, is a master regulator of the response to the jasmonate (JA) hormone in Arabidopsis (Arabidopsis thaliana). In the absence of JA, JASMONATE ZIM (JAZ) proteins interact with the MYC proteins to block their activity. Understanding of the mechanism and specificity of this interaction is key to unravel JA signalling. We generated mutant MYC proteins and assessed their activity and the specificity of their interaction with the 12 Arabidopsis JAZ proteins. We show that the D94N mutation present in the atr2D allele of MYC3 abolishes the interaction between MYC3 and most JAZ proteins. The same effect is observed when the corresponding conserved Asp (D105) was mutated in MYC2. Accordingly, MYC2(D105N) activated target genes in the presence of JAZ proteins, in contrast to wild-type MYC2. JAZ1 and JAZ10 were the only JAZ proteins still showing interaction with the mutant MYC proteins, due to a second MYC interaction domain, besides the classical Jas domain. Our results visualize the divergence among JAZ proteins in their interaction with MYC proteins. Ultimately, the transferability of the Asp-to-Asn amino acid change might facilitate the design of hyperactive transcription factors for plant engineering.},
  author       = {Goossens, Jonas and Swinnen, Gwen and Vanden Bossche, Robin and Pauwels, Laurens and Goossens, Alain},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keywords     = {Arabidopsis,abscisic acid (ABA),bHLH transcription factor,jasmonate (JA),JAZ,MYC2,transactivation,JASMONATE RESPONSES,GLUCOSINOLATE BIOSYNTHESIS,ARABIDOPSIS-THALIANA,GENE-EXPRESSION,SPLICE VARIANT,PROTEINS,DEFENSE,TARGETS,GROWTH,MOTIF},
  language     = {eng},
  number       = {4},
  pages        = {1229--1237},
  title        = {Change of a conserved amino acid in the MYC2 and MYC3 transcription factors leads to release of JAZ repression and increased activity},
  url          = {http://dx.doi.org/10.1111/nph.13398},
  volume       = {206},
  year         = {2015},
}

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