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Alpha-catenins control cardiomyocyte proliferation by regulating Yap activity

(2015) CIRCULATION RESEARCH. 116(1). p.70-79
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Abstract
Rationale: Shortly after birth, muscle cells of the mammalian heart lose their ability to divide. Thus, they are unable to effectively replace dying cells in the injured heart. The recent discovery that the transcriptional coactivator Yes-associated protein (Yap) is necessary and sufficient for cardiomyocyte proliferation has gained considerable attention. However, the upstream regulators and signaling pathways that control Yap activity in the heart are poorly understood. Objective: To investigate the role of alpha-catenins in the heart using cardiac-specific alpha E- and alpha T-catenin double knockout mice. Methods and Results: We used 2 cardiac-specific Cre transgenes to delete both alpha E-catenin (Ctnna1) and alpha T-catenin (Ctnna3) genes either in the perinatal or in the adult heart. Perinatal depletion of alpha-catenins increased cardiomyocyte number in the postnatal heart. Increased nuclear Yap and the cell cycle regulator cyclin D1 accompanied cardiomyocyte proliferation in the alpha-catenin double knockout hearts. Fetal genes were increased in the alpha-catenin double knockout hearts indicating a less mature cardiac gene expression profile. Knockdown of alpha-catenins in neonatal rat cardiomyocytes also resulted in increased proliferation, which could be blocked by knockdown of Yap. Finally, inactivation of alpha-catenins in the adult heart using an inducible Cre led to increased nuclear Yap and cardiomyocyte proliferation and improved contractility after myocardial infarction. Conclusions: These studies demonstrate that alpha-catenins are critical regulators of Yap, a transcriptional coactivator essential for cardiomyocyte proliferation. Furthermore, we provide proof of concept that inhibiting alpha-catenins might be a useful strategy to promote myocardial regeneration after injury.
Keywords
alpha-catenin, cytokinesis, models, animal, myocardial infarction, myocytes, cardiac, Yap protein, mouse, T-CATENIN, HIPPO PATHWAY, MYOCARDIAL-INFARCTION, SIGNALING PATHWAY, ADHERENS JUNCTION, TUMOR-SUPPRESSOR, AREA COMPOSITA, BETA-CATENIN, CELL-CELL, HEART

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Citation

Please use this url to cite or link to this publication:

Chicago
Li, Jifen, Erhe Gao, Alexia Vite, Roslyn Yi, Ludovic Gomez, Steven Goossens, Frans Van Roy, and Glenn L Radice. 2015. “Alpha-catenins Control Cardiomyocyte Proliferation by Regulating Yap Activity.” Circulation Research 116 (1): 70–79.
APA
Li, Jifen, Gao, E., Vite, A., Yi, R., Gomez, L., Goossens, S., Van Roy, F., et al. (2015). Alpha-catenins control cardiomyocyte proliferation by regulating Yap activity. CIRCULATION RESEARCH, 116(1), 70–79.
Vancouver
1.
Li J, Gao E, Vite A, Yi R, Gomez L, Goossens S, et al. Alpha-catenins control cardiomyocyte proliferation by regulating Yap activity. CIRCULATION RESEARCH. 2015;116(1):70–9.
MLA
Li, Jifen et al. “Alpha-catenins Control Cardiomyocyte Proliferation by Regulating Yap Activity.” CIRCULATION RESEARCH 116.1 (2015): 70–79. Print.
@article{5923459,
  abstract     = {Rationale: Shortly after birth, muscle cells of the mammalian heart lose their ability to divide. Thus, they are unable to effectively replace dying cells in the injured heart. The recent discovery that the transcriptional coactivator Yes-associated protein (Yap) is necessary and sufficient for cardiomyocyte proliferation has gained considerable attention. However, the upstream regulators and signaling pathways that control Yap activity in the heart are poorly understood.
Objective: To investigate the role of alpha-catenins in the heart using cardiac-specific alpha E- and alpha T-catenin double knockout mice.
Methods and Results: We used 2 cardiac-specific Cre transgenes to delete both alpha E-catenin (Ctnna1) and alpha T-catenin (Ctnna3) genes either in the perinatal or in the adult heart. Perinatal depletion of alpha-catenins increased cardiomyocyte number in the postnatal heart. Increased nuclear Yap and the cell cycle regulator cyclin D1 accompanied cardiomyocyte proliferation in the alpha-catenin double knockout hearts. Fetal genes were increased in the alpha-catenin double knockout hearts indicating a less mature cardiac gene expression profile. Knockdown of alpha-catenins in neonatal rat cardiomyocytes also resulted in increased proliferation, which could be blocked by knockdown of Yap. Finally, inactivation of alpha-catenins in the adult heart using an inducible Cre led to increased nuclear Yap and cardiomyocyte proliferation and improved contractility after myocardial infarction.
Conclusions: These studies demonstrate that alpha-catenins are critical regulators of Yap, a transcriptional coactivator essential for cardiomyocyte proliferation. Furthermore, we provide proof of concept that inhibiting alpha-catenins might be a useful strategy to promote myocardial regeneration after injury.},
  author       = {Li, Jifen and Gao, Erhe and Vite, Alexia and Yi, Roslyn and Gomez, Ludovic and Goossens, Steven and Van Roy, Frans and Radice, Glenn L},
  issn         = {0009-7330},
  journal      = {CIRCULATION RESEARCH},
  keywords     = {alpha-catenin,cytokinesis,models,animal,myocardial infarction,myocytes,cardiac,Yap protein,mouse,T-CATENIN,HIPPO PATHWAY,MYOCARDIAL-INFARCTION,SIGNALING PATHWAY,ADHERENS JUNCTION,TUMOR-SUPPRESSOR,AREA COMPOSITA,BETA-CATENIN,CELL-CELL,HEART},
  language     = {eng},
  number       = {1},
  pages        = {70--79},
  title        = {Alpha-catenins control cardiomyocyte proliferation by regulating Yap activity},
  url          = {http://dx.doi.org/10.1161/CIRCRESAHA.116.304472},
  volume       = {116},
  year         = {2015},
}

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