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Profiling and elucidation of the phenolic compounds in the aerial parts of Gynura bicolor and G. divaricata collected from different Chinese origins

(2015) CHEMISTRY & BIODIVERSITY. 12(1). p.96-115
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01SF0209 Special Research Fund Joint PhD
Abstract
Gynura bicolor and G. divaricata are not only known to be nutritive as cultured vegetables, but also beneficial as folk medicines in East Asia. As demonstrated by the current phytochemical knowledge, the genus Gynura is a promising source of phenolics with multiple medicinal activities. To expand this phytochemical knowledge, the phenolic secondary metabolites of G. bicolor and G. divaricata were studied. From the aerial parts of these two species, collected in five different Chinese locations, two fractions of phenolic compounds with different polarity were obtained by extraction and chromatographic separation. Using UPLC/MS/MS analysis, a total of 53 phenolics were either identified by comparison with respective reference compounds or tentatively characterized by their chromatographic behavior, UV-absorption patterns, and MS fragmentations. Some naturally existing positional isomers of O-caffeoylquinic acid, O-p-coumaroylquinic acid, O-feruloylquinic acid, and dicaffeoylquinic acid as well as their methyl esters were qualitatively characterized by their specific fragmentation patterns in targeted MS/MS. In addition, the aerial parts of the two Gynura species contained kaempferol, quercetin oligoglycosides, and a variety of derivatives of benzoic acid, hydroxycinnamic acid, and caffeic acid. Furthermore, the distribution of phenolic compounds in the two species from different Chinese origins was discussed. Finally, an investigation of the total phenolic content and in vitro antioxidant activity of the various phenolic fractions was completed, to evaluate the potential of the extracts of these species for medicinal development. The free-radical-scavenging activities of the extracts derived from plants originating from Nanjing were proven to be higher than those of the other extracts, which correlated well with their total phenolic content.
Keywords
LC-MSN, IDENTIFICATION, ANTIOXIDANT ACTIVITY, CONSTITUENTS, LEAVES, ACID, FRACTION, ISOMERS, RATS

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Citation

Please use this url to cite or link to this publication:

MLA
Chen, Jian et al. “Profiling and Elucidation of the Phenolic Compounds in the Aerial Parts of Gynura Bicolor and G. Divaricata Collected from Different Chinese Origins.” CHEMISTRY & BIODIVERSITY 12.1 (2015): 96–115. Print.
APA
Chen, Jian, Mangelinckx, S., Lu, H., Wang, Z.-T., Li, W.-L., & De Kimpe, N. (2015). Profiling and elucidation of the phenolic compounds in the aerial parts of Gynura bicolor and G. divaricata collected from different Chinese origins. CHEMISTRY & BIODIVERSITY, 12(1), 96–115.
Chicago author-date
Chen, Jian, Sven Mangelinckx, Han Lu, Zheng-Tao Wang, Wei-Lin Li, and Norbert De Kimpe. 2015. “Profiling and Elucidation of the Phenolic Compounds in the Aerial Parts of Gynura Bicolor and G. Divaricata Collected from Different Chinese Origins.” Chemistry & Biodiversity 12 (1): 96–115.
Chicago author-date (all authors)
Chen, Jian, Sven Mangelinckx, Han Lu, Zheng-Tao Wang, Wei-Lin Li, and Norbert De Kimpe. 2015. “Profiling and Elucidation of the Phenolic Compounds in the Aerial Parts of Gynura Bicolor and G. Divaricata Collected from Different Chinese Origins.” Chemistry & Biodiversity 12 (1): 96–115.
Vancouver
1.
Chen J, Mangelinckx S, Lu H, Wang Z-T, Li W-L, De Kimpe N. Profiling and elucidation of the phenolic compounds in the aerial parts of Gynura bicolor and G. divaricata collected from different Chinese origins. CHEMISTRY & BIODIVERSITY. 2015;12(1):96–115.
IEEE
[1]
J. Chen, S. Mangelinckx, H. Lu, Z.-T. Wang, W.-L. Li, and N. De Kimpe, “Profiling and elucidation of the phenolic compounds in the aerial parts of Gynura bicolor and G. divaricata collected from different Chinese origins,” CHEMISTRY & BIODIVERSITY, vol. 12, no. 1, pp. 96–115, 2015.
@article{5919394,
  abstract     = {Gynura bicolor and G. divaricata are not only known to be nutritive as cultured vegetables, but also beneficial as folk medicines in East Asia. As demonstrated by the current phytochemical knowledge, the genus Gynura is a promising source of phenolics with multiple medicinal activities. To expand this phytochemical knowledge, the phenolic secondary metabolites of G. bicolor and G. divaricata were studied. From the aerial parts of these two species, collected in five different Chinese locations, two fractions of phenolic compounds with different polarity were obtained by extraction and chromatographic separation. Using UPLC/MS/MS analysis, a total of 53 phenolics were either identified by comparison with respective reference compounds or tentatively characterized by their chromatographic behavior, UV-absorption patterns, and MS fragmentations. Some naturally existing positional isomers of O-caffeoylquinic acid, O-p-coumaroylquinic acid, O-feruloylquinic acid, and dicaffeoylquinic acid as well as their methyl esters were qualitatively characterized by their specific fragmentation patterns in targeted MS/MS. In addition, the aerial parts of the two Gynura species contained kaempferol, quercetin oligoglycosides, and a variety of derivatives of benzoic acid, hydroxycinnamic acid, and caffeic acid. Furthermore, the distribution of phenolic compounds in the two species from different Chinese origins was discussed. Finally, an investigation of the total phenolic content and in vitro antioxidant activity of the various phenolic fractions was completed, to evaluate the potential of the extracts of these species for medicinal development. The free-radical-scavenging activities of the extracts derived from plants originating from Nanjing were proven to be higher than those of the other extracts, which correlated well with their total phenolic content.},
  author       = {Chen, Jian and Mangelinckx, Sven and Lu, Han and Wang, Zheng-Tao and Li, Wei-Lin and De Kimpe, Norbert},
  issn         = {1612-1872},
  journal      = {CHEMISTRY & BIODIVERSITY},
  keywords     = {LC-MSN,IDENTIFICATION,ANTIOXIDANT ACTIVITY,CONSTITUENTS,LEAVES,ACID,FRACTION,ISOMERS,RATS},
  language     = {eng},
  number       = {1},
  pages        = {96--115},
  title        = {Profiling and elucidation of the phenolic compounds in the aerial parts of Gynura bicolor and G. divaricata collected from different Chinese origins},
  url          = {http://dx.doi.org/10.1002/cbdv.201400134},
  volume       = {12},
  year         = {2015},
}

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