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γ-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging

Charlot Vandevoorde, Caro Franck UGent, Klaus Bacher UGent, Luc Breysem, Maria Helena Smet, Adelard De Backer, Kris Van De Moortele, Peter Smeets and Hubert Thierens UGent (2015) EUROPEAN RADIOLOGY. 25(3). p.800-811
abstract
Objectives: Investigation of DNA damage induced by CT x-rays in paediatric patients versus patient dose in a multicentre setting. Methods: From 51 paediatric patients (median age, 3.8 years) who underwent an abdomen or chest CT examination in one of the five participating radiology departments, blood samples were taken before and shortly after the examination. DNA damage was estimated by scoring gamma-H2AX foci in peripheral blood T lymphocytes. Patient-specific organ and tissue doses were calculated with a validated Monte Carlo program. Individual lifetime attributable risks (LAR) for cancer incidence and mortality were estimated according to the BEIR VII risk models. Results: Despite the low CT doses, a median increase of 0.13 gamma-H2AX foci/cell was observed. Plotting the induced gamma-H2AX foci versus blood dose indicated a low-dose hypersensitivity, supported also by an in vitro dose-response study. Differences in dose levels between radiology centres were reflected in differences in DNA damage. LAR of cancer mortality for the paediatric chest CT and abdomen CT cohort was 0.08 and 0.13% respectively. Conclusion: CT x-rays induce DNA damage in paediatric patients even at low doses and the level of DNA damage is reduced by application of more effective CT dose reduction techniques and paediatric protocols.
Please use this url to cite or link to this publication:
author
organization
alternative title
gamma-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging
year
type
journalArticle (original)
publication status
published
subject
keyword
Double-strand DNA breaks, X-ray computed tomography, Radiobiology, gammaH2AX protein, Paediatrics, DOUBLE-STRAND BREAKS, RADIATION-EXPOSURE, COMPUTED-TOMOGRAPHY, IONIZING-RADIATION, BLOOD-LYMPHOCYTES, WHOLE-BLOOD, CANCER, REPAIR, REDUCTION, PROTOCOLS
journal title
EUROPEAN RADIOLOGY
Eur. Radiol.
volume
25
issue
3
pages
800 - 811
Web of Science type
Article
Web of Science id
000351226300025
JCR category
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
JCR impact factor
3.64 (2015)
JCR rank
18/124 (2015)
JCR quartile
1 (2015)
ISSN
0938-7994
DOI
10.1007/s00330-014-3463-8
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5919101
handle
http://hdl.handle.net/1854/LU-5919101
date created
2015-03-31 17:08:12
date last changed
2018-02-21 14:21:38
@article{5919101,
  abstract     = {Objectives: Investigation of DNA damage induced by CT x-rays in paediatric patients versus patient dose in a multicentre setting. 
Methods: From 51 paediatric patients (median age, 3.8 years) who underwent an abdomen or chest CT examination in one of the five participating radiology departments, blood samples were taken before and shortly after the examination. DNA damage was estimated by scoring gamma-H2AX foci in peripheral blood T lymphocytes. Patient-specific organ and tissue doses were calculated with a validated Monte Carlo program. Individual lifetime attributable risks (LAR) for cancer incidence and mortality were estimated according to the BEIR VII risk models. 
Results: Despite the low CT doses, a median increase of 0.13 gamma-H2AX foci/cell was observed. Plotting the induced gamma-H2AX foci versus blood dose indicated a low-dose hypersensitivity, supported also by an in vitro dose-response study. Differences in dose levels between radiology centres were reflected in differences in DNA damage. LAR of cancer mortality for the paediatric chest CT and abdomen CT cohort was 0.08 and 0.13\% respectively. 
Conclusion: CT x-rays induce DNA damage in paediatric patients even at low doses and the level of DNA damage is reduced by application of more effective CT dose reduction techniques and paediatric protocols.},
  author       = {Vandevoorde, Charlot and Franck, Caro and Bacher, Klaus and Breysem, Luc and Smet, Maria Helena and De Backer, Adelard and Van De Moortele, Kris and Smeets, Peter and Thierens, Hubert},
  issn         = {0938-7994},
  journal      = {EUROPEAN RADIOLOGY},
  keyword      = {Double-strand DNA breaks,X-ray computed tomography,Radiobiology,gammaH2AX protein,Paediatrics,DOUBLE-STRAND BREAKS,RADIATION-EXPOSURE,COMPUTED-TOMOGRAPHY,IONIZING-RADIATION,BLOOD-LYMPHOCYTES,WHOLE-BLOOD,CANCER,REPAIR,REDUCTION,PROTOCOLS},
  language     = {eng},
  number       = {3},
  pages        = {800--811},
  title        = {\ensuremath{\gamma}-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging},
  url          = {http://dx.doi.org/10.1007/s00330-014-3463-8},
  volume       = {25},
  year         = {2015},
}

Chicago
Vandevoorde, Charlot, Caro Franck, Klaus Bacher, Luc Breysem, Maria Helena Smet, Adelard De Backer, Kris Van De Moortele, Peter Smeets, and Hubert Thierens. 2015. “γ-H2AX Foci as in Vivo Effect Biomarker in Children Emphasize the Importance to Minimize X-ray Doses in Paediatric CT Imaging.” European Radiology 25 (3): 800–811.
APA
Vandevoorde, C., Franck, C., Bacher, K., Breysem, L., Smet, M. H., De Backer, A., Van De Moortele, K., et al. (2015). γ-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging. EUROPEAN RADIOLOGY, 25(3), 800–811.
Vancouver
1.
Vandevoorde C, Franck C, Bacher K, Breysem L, Smet MH, De Backer A, et al. γ-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging. EUROPEAN RADIOLOGY. 2015;25(3):800–11.
MLA
Vandevoorde, Charlot, Caro Franck, Klaus Bacher, et al. “γ-H2AX Foci as in Vivo Effect Biomarker in Children Emphasize the Importance to Minimize X-ray Doses in Paediatric CT Imaging.” EUROPEAN RADIOLOGY 25.3 (2015): 800–811. Print.