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Activation of auxin signalling counteracts photorespiratory H2O2-dependent cell death

Pavel Kerchev (UGent) , Per Mühlenbock (UGent) , Jordi Denecker (UGent) , Kris Morreel (UGent) , Frank Hoeberichts (UGent) , Katrien Van Der Kelen (UGent) , Michaël Vandorpe (UGent) , Long Nguyen (UGent) , Dominique Audenaert (UGent) and Frank Van Breusegem (UGent)
(2015) PLANT CELL AND ENVIRONMENT. 38(2). p.253-265
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
The high metabolic flux through photorespiration constitutes a significant part of the carbon cycle. Although the major enzymatic steps of the photorespiratory pathway are well characterized, little information is available on the functional significance of photorespiration beyond carbon recycling. Particularly important in this respect is the peroxisomal catalase activity which removes photorespiratory H2O2 generated during the oxidation of glycolate to glyoxylate, thus maintaining the cellular redox homeostasis governing the perception, integration and execution of stress responses. By performing a chemical screen, we identified 34 small molecules that alleviate the negative effects of photorespiration in Arabidopsis thaliana mutants lacking photorespiratory catalase (cat2). The chlorophyll fluorescence parameter photosystem II maximum efficiency (F-v/F-m) was used as a high-throughput readout. The most potent chemical that could rescue the photorespiratory phenotype of cat2 is a pro-auxin that contains a synthetic auxin-like substructure belonging to the phenoxy herbicide family, which can be released in planta. The naturally occurring indole-3-acetic acid (IAA) and other chemically distinct synthetic auxins also inhibited the photorespiratory-dependent cell death in cat2 mutants, implying a role for auxin signalling in stress tolerance. Although the photorespiratory pathway is biochemically well characterized, little information is available on the functional significance of photorespiration beyond carbon recycling. Particularly important in this respect is theperoxisomal catalase activity which removes photorespiratory H2O2.. By perfroming a chemical screen, we identified 34 small molecules that alleviate the negative effects of photorespiration in Arabidopsis thaliana mutants lacking photorespiratory catalase (cat2). The most potent chemical that could rescue the photorespiratory phenotype of cat2 is a pro-auxin structure, implying a role for auxin signaling in stress tolerance.
Keywords
INDUCED MORPHOGENIC RESPONSE, HYDROGEN-PEROXIDE, LATERAL ROOT-FORMATION, pro-auxin, photorespiration, chemical genetics, catalase, Arabidopsis, GENE-EXPRESSION, OXIDATIVE STRESS, ARABIDOPSIS, MUTANTS, PLANTS, REDOX, PHOTOSYNTHESIS

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Citation

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Chicago
Kerchev, Pavel, Per Mühlenbock, Jordi Denecker, Kris Morreel, Frank Hoeberichts, Katrien Van Der Kelen, Michaël Vandorpe, Long Nguyen, Dominique Audenaert, and Frank Van Breusegem. 2015. “Activation of Auxin Signalling Counteracts Photorespiratory H2O2-dependent Cell Death.” Plant Cell and Environment 38 (2): 253–265.
APA
Kerchev, P., Mühlenbock, P., Denecker, J., Morreel, K., Hoeberichts, F., Van Der Kelen, K., Vandorpe, M., et al. (2015). Activation of auxin signalling counteracts photorespiratory H2O2-dependent cell death. PLANT CELL AND ENVIRONMENT, 38(2), 253–265.
Vancouver
1.
Kerchev P, Mühlenbock P, Denecker J, Morreel K, Hoeberichts F, Van Der Kelen K, et al. Activation of auxin signalling counteracts photorespiratory H2O2-dependent cell death. PLANT CELL AND ENVIRONMENT. 2015;38(2):253–65.
MLA
Kerchev, Pavel, Per Mühlenbock, Jordi Denecker, et al. “Activation of Auxin Signalling Counteracts Photorespiratory H2O2-dependent Cell Death.” PLANT CELL AND ENVIRONMENT 38.2 (2015): 253–265. Print.
@article{5914220,
  abstract     = {The high metabolic flux through photorespiration constitutes a significant part of the carbon cycle. Although the major enzymatic steps of the photorespiratory pathway are well characterized, little information is available on the functional significance of photorespiration beyond carbon recycling. Particularly important in this respect is the peroxisomal catalase activity which removes photorespiratory H2O2 generated during the oxidation of glycolate to glyoxylate, thus maintaining the cellular redox homeostasis governing the perception, integration and execution of stress responses. By performing a chemical screen, we identified 34 small molecules that alleviate the negative effects of photorespiration in Arabidopsis thaliana mutants lacking photorespiratory catalase (cat2). The chlorophyll fluorescence parameter photosystem II maximum efficiency (F-v/F-m) was used as a high-throughput readout. The most potent chemical that could rescue the photorespiratory phenotype of cat2 is a pro-auxin that contains a synthetic auxin-like substructure belonging to the phenoxy herbicide family, which can be released in planta. The naturally occurring indole-3-acetic acid (IAA) and other chemically distinct synthetic auxins also inhibited the photorespiratory-dependent cell death in cat2 mutants, implying a role for auxin signalling in stress tolerance. 
Although the photorespiratory pathway is biochemically well characterized, little information is available on the functional significance of photorespiration beyond carbon recycling. Particularly important in this respect is theperoxisomal catalase activity which removes photorespiratory H2O2.. By perfroming a chemical screen, we identified 34 small molecules that alleviate the negative effects of photorespiration in Arabidopsis thaliana mutants lacking photorespiratory catalase (cat2). The most potent chemical that could rescue the photorespiratory phenotype of cat2 is a pro-auxin structure, implying a role for auxin signaling in stress tolerance.},
  author       = {Kerchev, Pavel and M{\"u}hlenbock, Per and Denecker, Jordi and Morreel, Kris and Hoeberichts, Frank and Van Der Kelen, Katrien and Vandorpe, Micha{\"e}l and Nguyen, Long and Audenaert, Dominique and Van Breusegem, Frank},
  issn         = {0140-7791},
  journal      = {PLANT CELL AND ENVIRONMENT},
  language     = {eng},
  number       = {2},
  pages        = {253--265},
  title        = {Activation of auxin signalling counteracts photorespiratory H2O2-dependent cell death},
  url          = {http://dx.doi.org/10.1111/pce.12250},
  volume       = {38},
  year         = {2015},
}

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