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In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers

Eva Vergucht (UGent) , Toon Brans (UGent) , Filip Beunis (UGent) , Jan Garrevoet (UGent) , Maarten De Rijcke (UGent) , Stephen Bauters (UGent) , David Deruytter (UGent) , Michiel Vandegehuchte (UGent) , Ine Van Nieuwenhove (UGent) , Colin Janssen (UGent) , et al.
Author
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Center for nano- and biophotonics (NB-Photonics)
Abstract
We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability.
Keywords
microalgae, single cell imaging, biological model organisms, confocal, X-ray fluorescence, In vivo, optical tweezers

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Chicago
Vergucht, Eva, Toon Brans, Filip Beunis, Jan Garrevoet, Maarten De Rijcke, Stephen Bauters, David Deruytter, et al. 2015. “In Vivo X-ray Elemental Imaging of Single Cell Model Organisms Manipulated by Laser-based Optical Tweezers.” Scientific Reports 5.
APA
Vergucht, E., Brans, T., Beunis, F., Garrevoet, J., De Rijcke, M., Bauters, S., Deruytter, D., et al. (2015). In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers. SCIENTIFIC REPORTS, 5.
Vancouver
1.
Vergucht E, Brans T, Beunis F, Garrevoet J, De Rijcke M, Bauters S, et al. In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers. SCIENTIFIC REPORTS. 2015;5.
MLA
Vergucht, Eva, Toon Brans, Filip Beunis, et al. “In Vivo X-ray Elemental Imaging of Single Cell Model Organisms Manipulated by Laser-based Optical Tweezers.” SCIENTIFIC REPORTS 5 (2015): n. pag. Print.
@article{5894087,
  abstract     = {We report on a radically new elemental imaging approach for the analysis of biological model organisms and single cells in their natural, in vivo state. The methodology combines optical tweezers (OT) technology for non-contact, laser based sample manipulation with synchrotron radiation confocal X-ray fluorescence (XRF) microimaging for the first time. The main objective of this work is to establish a new method for in vivo elemental imaging in a two-dimensional (2D) projection mode in free-standing biological microorganisms or single cells, present in their aqueous environment. Using the model organism Scrippsiella trochoidea, a first proof of principle experiment at beamline ID13 of the European Synchrotron Radiation Facility (ESRF) demonstrates the feasibility of the OT XRF methodology, which is applied to study mixture toxicity of Cu-Ni and Cu-Zn as a result of elevated exposure. We expect that the new OT XRF methodology will significantly contribute to the new trend of investigating microorganisms at the cellular level with added in vivo capability.},
  articleno    = {9049},
  author       = {Vergucht, Eva and Brans, Toon and Beunis, Filip and Garrevoet, Jan and De Rijcke, Maarten and Bauters, Stephen and Deruytter, David and Vandegehuchte, Michiel and Van Nieuwenhove, Ine and Janssen, Colin and Burghammer, Manfred and Vincze, Laszlo},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  language     = {eng},
  pages        = {8},
  title        = {In vivo X-ray elemental imaging of single cell model organisms manipulated by laser-based optical tweezers},
  url          = {http://dx.doi.org/10.1038/srep09049},
  volume       = {5},
  year         = {2015},
}

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