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Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense

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Abstract
Root nodule bacteria were isolated from Centrolobium paraense Tul. grown in soils from the Amazon region, State of Roraima (Brazil). 16S rRNA gene sequence analysis of seven strains (BR 10247(T), BR 10296, BR 10297, BR 10298, BR 10299, BR 10300 and BR 10301) placed them in the genus Bradyrhizobium with the closest neighbours being the type strains of Bradyrhizobium paxllaeri (98.8 % similarity), Bradyrhizobium icense (98.8 %), Bradyrhizobium lablabi (98.7 %), Bradyrhizobium jicamae (98.6 %), Bradyrhizobium elkanii (98.6 %), Bradyrhizobium pachyrhizi (98.6%) and Bradyrhizobium retamae (98.3 %). This high similarity, however, was not confirmed by the intergenic transcribed spacer (ITS) 16S-23S rRNA region sequence analysis nor by multi-locus sequence analysis. Phylogenetic analyses of five housekeeping genes (dnaK, gin/I, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EKO5(T) (=LMG 24129(T)) to. be the most closely related type strain (95.7% sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [major components being C-16:0 and summed feature 8 (18:1 omega 6c/18:1 omega 7c)] DNA G+C content, slow growth rate and carbon compound utilization patterns, supported the placement of the novel strains in the genus Bradyrhizobium. Results of DNA-DNA relatedness studies and physiological data (especially carbon source utilization) differentiated the strains from the closest recognized species of the genus Bradyrhizobium. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nil/-I) placed the novel species in a new branch within the genus Bradyrhizobium. Based on the current data, these seven strains represent a novel species for which the name Bradyrhizobium neotropicale sp. nov. is proposed. The type strain is BR 10247(T) (=HAMBI 3599(T)).
Keywords
STRAINS, RHIZOBIA, DEOXYRIBONUCLEIC-ACID, BRAZIL, SINORHIZOBIUM, LEGUME, DIVERSITY, MULTILOCUS SEQUENCE-ANALYSIS, SYMBIOTIC RELATIONSHIPS, DNA-DNA HYBRIDIZATION

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Citation

Please use this url to cite or link to this publication:

MLA
Zilli, Jerri E et al. “Bradyrhizobium Neotropicale Sp. Nov., Isolated from Effective Nodules of Centrolobium Paraense.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.12 (2014): 3950–3957. Print.
APA
Zilli, J. E., Baraúna, A. C., da Silva, K., De Meyer, S., Farias, E. N., Kaminski, P. E., da Costa, I. B., et al. (2014). Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(12), 3950–3957.
Chicago author-date
Zilli, Jerri E, Alexandre C Baraúna, Krisle da Silva, Sofie De Meyer, Eliane NC Farias, Paulo E Kaminski, Ismaele B da Costa, et al. 2014. “Bradyrhizobium Neotropicale Sp. Nov., Isolated from Effective Nodules of Centrolobium Paraense.” International Journal of Systematic and Evolutionary Microbiology 64 (12): 3950–3957.
Chicago author-date (all authors)
Zilli, Jerri E, Alexandre C Baraúna, Krisle da Silva, Sofie De Meyer, Eliane NC Farias, Paulo E Kaminski, Ismaele B da Costa, Julie K Ardley, Anne Willems, Natalia N Camacho, Fernanda dos S Dourado, and Graham O’Hara. 2014. “Bradyrhizobium Neotropicale Sp. Nov., Isolated from Effective Nodules of Centrolobium Paraense.” International Journal of Systematic and Evolutionary Microbiology 64 (12): 3950–3957.
Vancouver
1.
Zilli JE, Baraúna AC, da Silva K, De Meyer S, Farias EN, Kaminski PE, et al. Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(12):3950–7.
IEEE
[1]
J. E. Zilli et al., “Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 12, pp. 3950–3957, 2014.
@article{5889472,
  abstract     = {Root nodule bacteria were isolated from Centrolobium paraense Tul. grown in soils from the Amazon region, State of Roraima (Brazil). 16S rRNA gene sequence analysis of seven strains (BR 10247(T), BR 10296, BR 10297, BR 10298, BR 10299, BR 10300 and BR 10301) placed them in the genus Bradyrhizobium with the closest neighbours being the type strains of Bradyrhizobium paxllaeri (98.8 % similarity), Bradyrhizobium icense (98.8 %), Bradyrhizobium lablabi (98.7 %), Bradyrhizobium jicamae (98.6 %), Bradyrhizobium elkanii (98.6 %), Bradyrhizobium pachyrhizi (98.6%) and Bradyrhizobium retamae (98.3 %). This high similarity, however, was not confirmed by the intergenic transcribed spacer (ITS) 16S-23S rRNA region sequence analysis nor by multi-locus sequence analysis. Phylogenetic analyses of five housekeeping genes (dnaK, gin/I, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EKO5(T) (=LMG 24129(T)) to. be the most closely related type strain (95.7% sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [major components being C-16:0 and summed feature 8 (18:1 omega 6c/18:1 omega 7c)] DNA G+C content, slow growth rate and carbon compound utilization patterns, supported the placement of the novel strains in the genus Bradyrhizobium. Results of DNA-DNA relatedness studies and physiological data (especially carbon source utilization) differentiated the strains from the closest recognized species of the genus Bradyrhizobium. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nil/-I) placed the novel species in a new branch within the genus Bradyrhizobium. Based on the current data, these seven strains represent a novel species for which the name Bradyrhizobium neotropicale sp. nov. is proposed. The type strain is BR 10247(T) (=HAMBI 3599(T)).},
  author       = {Zilli, Jerri E and Baraúna, Alexandre C and da Silva, Krisle and De Meyer, Sofie and Farias, Eliane NC and Kaminski, Paulo E and da Costa, Ismaele B and Ardley, Julie K and Willems, Anne and Camacho, Natalia N and Dourado, Fernanda dos S and O'Hara, Graham},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {STRAINS,RHIZOBIA,DEOXYRIBONUCLEIC-ACID,BRAZIL,SINORHIZOBIUM,LEGUME,DIVERSITY,MULTILOCUS SEQUENCE-ANALYSIS,SYMBIOTIC RELATIONSHIPS,DNA-DNA HYBRIDIZATION},
  language     = {eng},
  number       = {12},
  pages        = {3950--3957},
  title        = {Bradyrhizobium neotropicale sp. nov., isolated from effective nodules of Centrolobium paraense},
  url          = {http://dx.doi.org/10.1099/ijs.0.065458-0},
  volume       = {64},
  year         = {2014},
}

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