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Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis

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Abstract
Objectives: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting. Methods: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were cultured in parallel on simple Middlebrook 7H9, manual MGIT and Ogawa. A case of SN-TB was defined as one with a positive culture in any medium. Results: Among samples from 542 patients, 151 (28%) cases of SN-TB were identified. The sensitivity of Middlebrook 7H9 (0.76, 95% CI 0.69-0.83) was not substantially different from that of MGIT (0.85, 95% CI 0.79-0.91). Ogawa had the lowest sensitivity (0.63, 95% CI 0.55-0.71). The median turnaround time was similar for both liquid media (18days), and it was shorter than that of Ogawa (30days). Conclusions: Culture on simple Middlebrook 7H9 performs almost as well as MGIT, at a probably more affordable cost. Further studies on the cost-effectiveness of this overlooked technique should be performed.
Keywords
smear-negative tuberculosis, diagnosis, Middlebrook 7H9, MGIT, Ogawa, M, tuberculosis, diagnostic, tuberculose a frottis negatif, Middlebrook 7H9, MGIT, Ogawa, M, tuberculosis, diagnostico, tuberculosis con baciloscopia negativa, Ogawa, mycobacterial growth indicator tube, Middlebrook 7H9, Mycobacterium tuberculosis, GROWTH INDICATOR TUBE, SOLID MEDIA, BACTEC, MYCOBACTERIA

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Chicago
Battaglioli, T, A Soto, J Agapito, V Acurio, and Patrick Van Der Stuyft. 2014. “Manual Liquid Culture on Simple Middlebrook 7H9 or MGIT for the Diagnosis of Smear-negative Pulmonary Tuberculosis.” Tropical Medicine & International Health 19 (12): 1500–1503.
APA
Battaglioli, T., Soto, A., Agapito, J., Acurio, V., & Van Der Stuyft, P. (2014). Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 19(12), 1500–1503.
Vancouver
1.
Battaglioli T, Soto A, Agapito J, Acurio V, Van Der Stuyft P. Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis. TROPICAL MEDICINE & INTERNATIONAL HEALTH. 2014;19(12):1500–3.
MLA
Battaglioli, T, A Soto, J Agapito, et al. “Manual Liquid Culture on Simple Middlebrook 7H9 or MGIT for the Diagnosis of Smear-negative Pulmonary Tuberculosis.” TROPICAL MEDICINE & INTERNATIONAL HEALTH 19.12 (2014): 1500–1503. Print.
@article{5864439,
  abstract     = {Objectives: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting. 
Methods: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were cultured in parallel on simple Middlebrook 7H9, manual MGIT and Ogawa. A case of SN-TB was defined as one with a positive culture in any medium. 
Results: Among samples from 542 patients, 151 (28\%) cases of SN-TB were identified. The sensitivity of Middlebrook 7H9 (0.76, 95\% CI 0.69-0.83) was not substantially different from that of MGIT (0.85, 95\% CI 0.79-0.91). Ogawa had the lowest sensitivity (0.63, 95\% CI 0.55-0.71). The median turnaround time was similar for both liquid media (18days), and it was shorter than that of Ogawa (30days). 
Conclusions: Culture on simple Middlebrook 7H9 performs almost as well as MGIT, at a probably more affordable cost. Further studies on the cost-effectiveness of this overlooked technique should be performed.},
  author       = {Battaglioli, T and Soto, A and Agapito, J and Acurio, V and Van Der Stuyft, Patrick},
  issn         = {1360-2276},
  journal      = {TROPICAL MEDICINE \& INTERNATIONAL HEALTH},
  language     = {eng},
  number       = {12},
  pages        = {1500--1503},
  title        = {Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis},
  url          = {http://dx.doi.org/10.1111/tmi.12384},
  volume       = {19},
  year         = {2014},
}

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