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Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis

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Abstract
Objectives: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting. Methods: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were cultured in parallel on simple Middlebrook 7H9, manual MGIT and Ogawa. A case of SN-TB was defined as one with a positive culture in any medium. Results: Among samples from 542 patients, 151 (28%) cases of SN-TB were identified. The sensitivity of Middlebrook 7H9 (0.76, 95% CI 0.69-0.83) was not substantially different from that of MGIT (0.85, 95% CI 0.79-0.91). Ogawa had the lowest sensitivity (0.63, 95% CI 0.55-0.71). The median turnaround time was similar for both liquid media (18days), and it was shorter than that of Ogawa (30days). Conclusions: Culture on simple Middlebrook 7H9 performs almost as well as MGIT, at a probably more affordable cost. Further studies on the cost-effectiveness of this overlooked technique should be performed.
Keywords
smear-negative tuberculosis, diagnosis, Middlebrook 7H9, MGIT, Ogawa, M, tuberculosis, diagnostic, tuberculose a frottis negatif, Middlebrook 7H9, MGIT, Ogawa, M, tuberculosis, diagnostico, tuberculosis con baciloscopia negativa, Ogawa, mycobacterial growth indicator tube, Middlebrook 7H9, Mycobacterium tuberculosis, GROWTH INDICATOR TUBE, SOLID MEDIA, BACTEC, MYCOBACTERIA

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MLA
Battaglioli, T et al. “Manual Liquid Culture on Simple Middlebrook 7H9 or MGIT for the Diagnosis of Smear-negative Pulmonary Tuberculosis.” TROPICAL MEDICINE & INTERNATIONAL HEALTH 19.12 (2014): 1500–1503. Print.
APA
Battaglioli, T., Soto, A., Agapito, J., Acurio, V., & Van Der Stuyft, P. (2014). Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 19(12), 1500–1503.
Chicago author-date
Battaglioli, T, A Soto, J Agapito, V Acurio, and Patrick Van Der Stuyft. 2014. “Manual Liquid Culture on Simple Middlebrook 7H9 or MGIT for the Diagnosis of Smear-negative Pulmonary Tuberculosis.” Tropical Medicine & International Health 19 (12): 1500–1503.
Chicago author-date (all authors)
Battaglioli, T, A Soto, J Agapito, V Acurio, and Patrick Van Der Stuyft. 2014. “Manual Liquid Culture on Simple Middlebrook 7H9 or MGIT for the Diagnosis of Smear-negative Pulmonary Tuberculosis.” Tropical Medicine & International Health 19 (12): 1500–1503.
Vancouver
1.
Battaglioli T, Soto A, Agapito J, Acurio V, Van Der Stuyft P. Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis. TROPICAL MEDICINE & INTERNATIONAL HEALTH. 2014;19(12):1500–3.
IEEE
[1]
T. Battaglioli, A. Soto, J. Agapito, V. Acurio, and P. Van Der Stuyft, “Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis,” TROPICAL MEDICINE & INTERNATIONAL HEALTH, vol. 19, no. 12, pp. 1500–1503, 2014.
@article{5864439,
  abstract     = {Objectives: To compare the performance of liquid culture on simple Middlebrook 7H9 to the one of manual mycobacterial growth indicator tube (MGIT) and solid culture on Ogawa for the diagnosis of smear-negative tuberculosis (SN-TB) in a high-burden, resource-constrained setting. 
Methods: Sputum samples from patients with clinical suspicion of SN-PTB admitted to two-third-level hospitals in Lima between September 2005 and May 2008 were cultured in parallel on simple Middlebrook 7H9, manual MGIT and Ogawa. A case of SN-TB was defined as one with a positive culture in any medium. 
Results: Among samples from 542 patients, 151 (28%) cases of SN-TB were identified. The sensitivity of Middlebrook 7H9 (0.76, 95% CI 0.69-0.83) was not substantially different from that of MGIT (0.85, 95% CI 0.79-0.91). Ogawa had the lowest sensitivity (0.63, 95% CI 0.55-0.71). The median turnaround time was similar for both liquid media (18days), and it was shorter than that of Ogawa (30days). 
Conclusions: Culture on simple Middlebrook 7H9 performs almost as well as MGIT, at a probably more affordable cost. Further studies on the cost-effectiveness of this overlooked technique should be performed.},
  author       = {Battaglioli, T and Soto, A and Agapito, J and Acurio, V and Van Der Stuyft, Patrick},
  issn         = {1360-2276},
  journal      = {TROPICAL MEDICINE & INTERNATIONAL HEALTH},
  keywords     = {smear-negative tuberculosis,diagnosis,Middlebrook 7H9,MGIT,Ogawa,M,tuberculosis,diagnostic,tuberculose a frottis negatif,Middlebrook 7H9,MGIT,Ogawa,M,tuberculosis,diagnostico,tuberculosis con baciloscopia negativa,Ogawa,mycobacterial growth indicator tube,Middlebrook 7H9,Mycobacterium tuberculosis,GROWTH INDICATOR TUBE,SOLID MEDIA,BACTEC,MYCOBACTERIA},
  language     = {eng},
  number       = {12},
  pages        = {1500--1503},
  title        = {Manual liquid culture on simple Middlebrook 7H9 or MGIT for the diagnosis of smear-negative pulmonary tuberculosis},
  url          = {http://dx.doi.org/10.1111/tmi.12384},
  volume       = {19},
  year         = {2014},
}

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