Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity
- Author
- U Uladzimir Karniychuk and Hans Nauwynck (UGent)
- Organization
- Abstract
- Porcine reproductive and respiratory syndrome is the most economically important viral disease in the swine industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) strains are classified into two distinct genotypes, the European genotype and the North American genotype. The European PRRSV genotype has been divided into three subtypes: a pan-European subtype 1 and East European subtypes 2 and 3. The aim of this study was to evaluate the performance of commercial and homemade serological assays to test field sera from a geographical region with an extreme PRRSV heterogeneity. Belarus became the country of choice for sample collection because heterologous PRRSV strains of all known European subtypes circulate in this country. Sera from Belarusian swine farms were tested in immunoperoxidase monolayer assays based on pan-European subtype 1, East European subtype 3 and North American strains as antigens and commercial enzyme-linked immunosorbent assays (IDEXX and INGEZIM). The obtained results suggest that none of the serological tools for PRRSV diagnosis can guarantee a flawless detection of antibodies at the individual animal level. Considering heterogeneity of recently isolated European PRRSV strains the problem can be relevant in many countries.
- Keywords
- Belarus, SYNDROME SIRS VIRUS, diagnosis, serology, porcine reproductive and respiratory syndrome virus, SUBTYPES, INFECTION, LELYSTAD VIRUS, EASTERN-EUROPE, STRAINS, DIVERSITY, IDENTIFICATION, INFERTILITY, DEFINITION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-5862355
- MLA
- Karniychuk, U. Uladzimir, and Hans Nauwynck. “Performance of Assays for Testing Antibodies against Porcine Reproductive and Respiratory Syndrome Virus in Sera Collected from Swine Farms in a Region with an Extreme Virus Heterogeneity.” ACTA VETERINARIA-BEOGRAD, vol. 64, no. 1, 2014, pp. 44–51, doi:10.2478/acve-2014-0005.
- APA
- Karniychuk, U. U., & Nauwynck, H. (2014). Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity. ACTA VETERINARIA-BEOGRAD, 64(1), 44–51. https://doi.org/10.2478/acve-2014-0005
- Chicago author-date
- Karniychuk, U Uladzimir, and Hans Nauwynck. 2014. “Performance of Assays for Testing Antibodies against Porcine Reproductive and Respiratory Syndrome Virus in Sera Collected from Swine Farms in a Region with an Extreme Virus Heterogeneity.” ACTA VETERINARIA-BEOGRAD 64 (1): 44–51. https://doi.org/10.2478/acve-2014-0005.
- Chicago author-date (all authors)
- Karniychuk, U Uladzimir, and Hans Nauwynck. 2014. “Performance of Assays for Testing Antibodies against Porcine Reproductive and Respiratory Syndrome Virus in Sera Collected from Swine Farms in a Region with an Extreme Virus Heterogeneity.” ACTA VETERINARIA-BEOGRAD 64 (1): 44–51. doi:10.2478/acve-2014-0005.
- Vancouver
- 1.Karniychuk UU, Nauwynck H. Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity. ACTA VETERINARIA-BEOGRAD. 2014;64(1):44–51.
- IEEE
- [1]U. U. Karniychuk and H. Nauwynck, “Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity,” ACTA VETERINARIA-BEOGRAD, vol. 64, no. 1, pp. 44–51, 2014.
@article{5862355,
abstract = {{Porcine reproductive and respiratory syndrome is the most economically important viral disease in the swine industry worldwide. Porcine reproductive and respiratory syndrome virus (PRRSV) strains are classified into two distinct genotypes, the European genotype and the North American genotype. The European PRRSV genotype has been divided into three subtypes: a pan-European subtype 1 and East European subtypes 2 and 3. The aim of this study was to evaluate the performance of commercial and homemade serological assays to test field sera from a geographical region with an extreme PRRSV heterogeneity. Belarus became the country of choice for sample collection because heterologous PRRSV strains of all known European subtypes circulate in this country. Sera from Belarusian swine farms were tested in immunoperoxidase monolayer assays based on pan-European subtype 1, East European subtype 3 and North American strains as antigens and commercial enzyme-linked immunosorbent assays (IDEXX and INGEZIM). The obtained results suggest that none of the serological tools for PRRSV diagnosis can guarantee a flawless detection of antibodies at the individual animal level. Considering heterogeneity of recently isolated European PRRSV strains the problem can be relevant in many countries.}},
author = {{Karniychuk, U Uladzimir and Nauwynck, Hans}},
issn = {{0567-8315}},
journal = {{ACTA VETERINARIA-BEOGRAD}},
keywords = {{Belarus,SYNDROME SIRS VIRUS,diagnosis,serology,porcine reproductive and respiratory syndrome virus,SUBTYPES,INFECTION,LELYSTAD VIRUS,EASTERN-EUROPE,STRAINS,DIVERSITY,IDENTIFICATION,INFERTILITY,DEFINITION}},
language = {{eng}},
number = {{1}},
pages = {{44--51}},
title = {{Performance of assays for testing antibodies against porcine reproductive and respiratory syndrome virus in sera collected from swine farms in a region with an extreme virus heterogeneity}},
url = {{http://doi.org/10.2478/acve-2014-0005}},
volume = {{64}},
year = {{2014}},
}
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