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Photonic detection and characterization of DNA using sapphire microspheres

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Center for nano- and biophotonics (NB-Photonics)
Abstract
A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500 mu m, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q approximate to 10(4)) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Keywords
whispering gallery modes, sapphire microsphere, WHISPERING-GALLERY-MODE, MICRORING RESONATOR, OPTICAL BIOSENSORS, DIAMOND, SHIFT, SURFACES, SENSOR, MICROCAVITY, SENSITIVITY. deoxyribonucleic acid, label-free biosensor, microcavity, photonics, quality factor, LABEL-FREE DETECTION

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Citation

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MLA
Murib, Mohammed Sharif, Weng-Siang Yeap, Daan Martens, et al. “Photonic Detection and Characterization of DNA Using Sapphire Microspheres.” JOURNAL OF BIOMEDICAL OPTICS 19.9 (2014): n. pag. Print.
APA
Murib, M. S., Yeap, W.-S., Martens, D., Bienstman, P., De Ceuninck, W., van Grinsven, B., Schoening, M. J., et al. (2014). Photonic detection and characterization of DNA using sapphire microspheres. JOURNAL OF BIOMEDICAL OPTICS, 19(9).
Chicago author-date
Murib, Mohammed Sharif, Weng-Siang Yeap, Daan Martens, Peter Bienstman, Ward De Ceuninck, Bart van Grinsven, Michael J Schoening, et al. 2014. “Photonic Detection and Characterization of DNA Using Sapphire Microspheres.” Journal of Biomedical Optics 19 (9).
Chicago author-date (all authors)
Murib, Mohammed Sharif, Weng-Siang Yeap, Daan Martens, Peter Bienstman, Ward De Ceuninck, Bart van Grinsven, Michael J Schoening, Luc Michiels, Ken Haenen, Marcel Ameloot, Ali Serpenguzel, and Patrick Wagner. 2014. “Photonic Detection and Characterization of DNA Using Sapphire Microspheres.” Journal of Biomedical Optics 19 (9).
Vancouver
1.
Murib MS, Yeap W-S, Martens D, Bienstman P, De Ceuninck W, van Grinsven B, et al. Photonic detection and characterization of DNA using sapphire microspheres. JOURNAL OF BIOMEDICAL OPTICS. 2014;19(9).
IEEE
[1]
M. S. Murib et al., “Photonic detection and characterization of DNA using sapphire microspheres,” JOURNAL OF BIOMEDICAL OPTICS, vol. 19, no. 9, 2014.
@article{5847253,
  abstract     = {A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500 mu m, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q approximate to 10(4)) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)},
  articleno    = {097006},
  author       = {Murib, Mohammed Sharif and Yeap, Weng-Siang and Martens, Daan and Bienstman, Peter and De Ceuninck, Ward and van Grinsven, Bart and Schoening, Michael J and Michiels, Luc and Haenen, Ken and Ameloot, Marcel and Serpenguzel, Ali and Wagner, Patrick},
  issn         = {1083-3668},
  journal      = {JOURNAL OF BIOMEDICAL OPTICS},
  keywords     = {whispering gallery modes,sapphire microsphere,WHISPERING-GALLERY-MODE,MICRORING RESONATOR,OPTICAL BIOSENSORS,DIAMOND,SHIFT,SURFACES,SENSOR,MICROCAVITY,SENSITIVITY. deoxyribonucleic acid,label-free biosensor,microcavity,photonics,quality factor,LABEL-FREE DETECTION},
  language     = {eng},
  number       = {9},
  pages        = {8},
  title        = {Photonic detection and characterization of DNA using sapphire microspheres},
  url          = {http://dx.doi.org/10.1117/1.JBO.19.9.097006},
  volume       = {19},
  year         = {2014},
}

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