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GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis

(2015) EMBO JOURNAL. 34(1). p.55-66
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Recognition of extracellular peptides by plasma membrane-localized receptor proteins is commonly used in signal transduction. In plants, very little is known about how extracellular peptides are processed and activated in order to allow recognition by receptors. Here, we show that induction of cell death in planta by a secreted plant protein GRIM REAPER (GRI) is dependent on the activity of the type II metacaspase METACASPASE-9. GRI is cleaved by METACASPASE-9 in vitro resulting in the release of an 11 amino acid peptide. This peptide bound in vivo to the extracellular domain of the plasma membrane-localized, atypical leucine-rich repeat receptor-like kinase POLLEN-SPECIFIC RECEPTOR-LIKE KINASE 5 (PRK5) and was sufficient to induce oxidative stress/ROS-dependent cell death. This shows a signaling pathway in plants from processing and activation of an extracellular protein to recognition by its receptor.
Keywords
OXIDATIVE STRESS, PROTEIN-STRUCTURE, POLLEN-TUBE GROWTH, PATTERN-RECOGNITION RECEPTORS, secreted protein, receptor-like kinase, protease, ligand, PLANT PROTEASES, THALIANA, METACASPASES, ROS, PERCEPTION, ACTIVATION

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Citation

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MLA
Wrzaczek, Michael, Julia P Vainonen, Simon Stael, et al. “GRIM REAPER Peptide Binds to Receptor Kinase PRK5 to Trigger Cell Death in Arabidopsis.” EMBO JOURNAL 34.1 (2015): 55–66. Print.
APA
Wrzaczek, M., Vainonen, J. P., Stael, S., Tsiatsiani, L., Help-Rinta-Rahko, H., Gauthier, A., Kaufholdt, D., et al. (2015). GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis. EMBO JOURNAL, 34(1), 55–66.
Chicago author-date
Wrzaczek, Michael, Julia P Vainonen, Simon Stael, Liana Tsiatsiani, Hanna Help-Rinta-Rahko, Adrien Gauthier, David Kaufholdt, et al. 2015. “GRIM REAPER Peptide Binds to Receptor Kinase PRK5 to Trigger Cell Death in Arabidopsis.” Embo Journal 34 (1): 55–66.
Chicago author-date (all authors)
Wrzaczek, Michael, Julia P Vainonen, Simon Stael, Liana Tsiatsiani, Hanna Help-Rinta-Rahko, Adrien Gauthier, David Kaufholdt, Benjamin Bollhöner, Airi Lamminmäki, An Staes, Kris Gevaert, Hannele Tuominen, Frank Van Breusegem, Ykä Helariutta, and Jaakko Kangasjärvi. 2015. “GRIM REAPER Peptide Binds to Receptor Kinase PRK5 to Trigger Cell Death in Arabidopsis.” Embo Journal 34 (1): 55–66.
Vancouver
1.
Wrzaczek M, Vainonen JP, Stael S, Tsiatsiani L, Help-Rinta-Rahko H, Gauthier A, et al. GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis. EMBO JOURNAL. 2015;34(1):55–66.
IEEE
[1]
M. Wrzaczek et al., “GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis,” EMBO JOURNAL, vol. 34, no. 1, pp. 55–66, 2015.
@article{5846236,
  abstract     = {Recognition of extracellular peptides by plasma membrane-localized receptor proteins is commonly used in signal transduction. In plants, very little is known about how extracellular peptides are processed and activated in order to allow recognition by receptors. Here, we show that induction of cell death in planta by a secreted plant protein GRIM REAPER (GRI) is dependent on the activity of the type II metacaspase METACASPASE-9. GRI is cleaved by METACASPASE-9 in vitro resulting in the release of an 11 amino acid peptide. This peptide bound in vivo to the extracellular domain of the plasma membrane-localized, atypical leucine-rich repeat receptor-like kinase POLLEN-SPECIFIC RECEPTOR-LIKE KINASE 5 (PRK5) and was sufficient to induce oxidative stress/ROS-dependent cell death. This shows a signaling pathway in plants from processing and activation of an extracellular protein to recognition by its receptor.},
  author       = {Wrzaczek, Michael and Vainonen, Julia P and Stael, Simon and Tsiatsiani, Liana and Help-Rinta-Rahko, Hanna and Gauthier, Adrien and Kaufholdt, David and Bollhöner, Benjamin and Lamminmäki, Airi and Staes, An and Gevaert, Kris and Tuominen, Hannele and Van Breusegem, Frank and Helariutta, Ykä and Kangasjärvi, Jaakko},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keywords     = {OXIDATIVE STRESS,PROTEIN-STRUCTURE,POLLEN-TUBE GROWTH,PATTERN-RECOGNITION RECEPTORS,secreted protein,receptor-like kinase,protease,ligand,PLANT PROTEASES,THALIANA,METACASPASES,ROS,PERCEPTION,ACTIVATION},
  language     = {eng},
  number       = {1},
  pages        = {55--66},
  title        = {GRIM REAPER peptide binds to receptor kinase PRK5 to trigger cell death in Arabidopsis},
  url          = {http://dx.doi.org/10.15252/embj.201488582},
  volume       = {34},
  year         = {2015},
}

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