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Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts

Seppe De Smet, Bram Miserez, Maria Rambla Alegre, Mehmet Talha Yapa, André de Villiers, Patrick Sandra UGent and Frederic Lynen UGent (2015) JOURNAL OF SEPARATION SCIENCE. 38(5). p.724-731
abstract
This paper reports the optimization of the online coupling of 2,2’-azino-bis(3-ethylbenzothiazoline)-6 sulfonic acid based radical scavenging assays with reversed phase HPLC. The residence times in the reactor was reduced down to 6.4 seconds to ensure minimal peak broadening and loss of separation. Peak capacity losses between compound detection and measurement of the radical scavenging potential were reduced to 10% and lower on coupled column systems. The methodology was successfully applied for the detection of the scavenging activity of molecules encompassing a broad hydrophobicity range. The method shows promise for the assessment of low molecular weight polyphenols in red wine via coupled column high resolution HPLC-MS analysis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Wine, PHENOLIC-COMPOUNDS, Radicals, High-performance liquid chromatography, Antioxidants, HPLC, IDENTIFICATION, CAPACITY, CATION, WINES, CHEMILUMINESCENCE, POLYPHENOLS, METABOLITES, CHEMISTRY
journal title
JOURNAL OF SEPARATION SCIENCE
J. Sep. Sci.
volume
38
issue
5
pages
724 - 731
Web of Science type
Article
Web of Science id
000350201900004
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
2.741 (2015)
JCR rank
21/75 (2015)
JCR quartile
2 (2015)
ISSN
1615-9306
DOI
10.1002/jssc.201401222
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5845680
handle
http://hdl.handle.net/1854/LU-5845680
date created
2015-02-11 12:36:14
date last changed
2016-12-19 15:39:43
@article{5845680,
  abstract     = {This paper reports the optimization of the online coupling of 2,2{\textquoteright}-azino-bis(3-ethylbenzothiazoline)-6 sulfonic acid based radical scavenging assays with reversed  phase HPLC. The residence times in the reactor was reduced down to 6.4 seconds to ensure minimal peak broadening and loss of separation. Peak capacity losses between compound detection and measurement of the radical scavenging potential were reduced to 10\% and lower on coupled column systems. The methodology was successfully applied for the detection of the scavenging activity of molecules encompassing a broad hydrophobicity range. The method shows promise for the assessment of low molecular weight polyphenols in red wine via coupled column high resolution HPLC-MS analysis.},
  author       = {De Smet, Seppe and Miserez, Bram and Rambla Alegre, Maria and Yapa, Mehmet Talha and de Villiers, Andr{\'e} and Sandra, Patrick and Lynen, Frederic},
  issn         = {1615-9306},
  journal      = {JOURNAL OF SEPARATION SCIENCE},
  keyword      = {Wine,PHENOLIC-COMPOUNDS,Radicals,High-performance liquid chromatography,Antioxidants,HPLC,IDENTIFICATION,CAPACITY,CATION,WINES,CHEMILUMINESCENCE,POLYPHENOLS,METABOLITES,CHEMISTRY},
  language     = {eng},
  number       = {5},
  pages        = {724--731},
  title        = {Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts},
  url          = {http://dx.doi.org/10.1002/jssc.201401222},
  volume       = {38},
  year         = {2015},
}

Chicago
De Smet, Seppe, Bram Miserez, Maria Rambla Alegre, Mehmet Talha Yapa, André de Villiers, Patrick Sandra, and Frederic Lynen. 2015. “Optimization of a High-resolution Radical Scavenging Assay Coupled On-line to Reversed-phase Liquid Chromatography for Antioxidant Detection in Complex Natural Extracts.” Journal of Separation Science 38 (5): 724–731.
APA
De Smet, Seppe, Miserez, B., Rambla Alegre, M., Yapa, M. T., de Villiers, A., Sandra, P., & Lynen, F. (2015). Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts. JOURNAL OF SEPARATION SCIENCE, 38(5), 724–731.
Vancouver
1.
De Smet S, Miserez B, Rambla Alegre M, Yapa MT, de Villiers A, Sandra P, et al. Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts. JOURNAL OF SEPARATION SCIENCE. 2015;38(5):724–31.
MLA
De Smet, Seppe, Bram Miserez, Maria Rambla Alegre, et al. “Optimization of a High-resolution Radical Scavenging Assay Coupled On-line to Reversed-phase Liquid Chromatography for Antioxidant Detection in Complex Natural Extracts.” JOURNAL OF SEPARATION SCIENCE 38.5 (2015): 724–731. Print.