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Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts

Seppe De Smet, Bram Miserez, Maria Rambla Alegre, Mehmet Talha Yapa, André de Villiers, Patrick Sandra (UGent) and Frederic Lynen (UGent)
(2015) JOURNAL OF SEPARATION SCIENCE. 38(5). p.724-731
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Abstract
This paper reports the optimization of the online coupling of 2,2’-azino-bis(3-ethylbenzothiazoline)-6 sulfonic acid based radical scavenging assays with reversed phase HPLC. The residence times in the reactor was reduced down to 6.4 seconds to ensure minimal peak broadening and loss of separation. Peak capacity losses between compound detection and measurement of the radical scavenging potential were reduced to 10% and lower on coupled column systems. The methodology was successfully applied for the detection of the scavenging activity of molecules encompassing a broad hydrophobicity range. The method shows promise for the assessment of low molecular weight polyphenols in red wine via coupled column high resolution HPLC-MS analysis.
Keywords
Wine, PHENOLIC-COMPOUNDS, Radicals, High-performance liquid chromatography, Antioxidants, HPLC, IDENTIFICATION, CAPACITY, CATION, WINES, CHEMILUMINESCENCE, POLYPHENOLS, METABOLITES, CHEMISTRY

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MLA
De Smet, Seppe, et al. “Optimization of a High-Resolution Radical Scavenging Assay Coupled on-Line to Reversed-Phase Liquid Chromatography for Antioxidant Detection in Complex Natural Extracts.” JOURNAL OF SEPARATION SCIENCE, vol. 38, no. 5, 2015, pp. 724–31, doi:10.1002/jssc.201401222.
APA
De Smet, S., Miserez, B., Rambla Alegre, M., Yapa, M. T., de Villiers, A., Sandra, P., & Lynen, F. (2015). Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts. JOURNAL OF SEPARATION SCIENCE, 38(5), 724–731. https://doi.org/10.1002/jssc.201401222
Chicago author-date
De Smet, Seppe, Bram Miserez, Maria Rambla Alegre, Mehmet Talha Yapa, André de Villiers, Patrick Sandra, and Frederic Lynen. 2015. “Optimization of a High-Resolution Radical Scavenging Assay Coupled on-Line to Reversed-Phase Liquid Chromatography for Antioxidant Detection in Complex Natural Extracts.” JOURNAL OF SEPARATION SCIENCE 38 (5): 724–31. https://doi.org/10.1002/jssc.201401222.
Chicago author-date (all authors)
De Smet, Seppe, Bram Miserez, Maria Rambla Alegre, Mehmet Talha Yapa, André de Villiers, Patrick Sandra, and Frederic Lynen. 2015. “Optimization of a High-Resolution Radical Scavenging Assay Coupled on-Line to Reversed-Phase Liquid Chromatography for Antioxidant Detection in Complex Natural Extracts.” JOURNAL OF SEPARATION SCIENCE 38 (5): 724–731. doi:10.1002/jssc.201401222.
Vancouver
1.
De Smet S, Miserez B, Rambla Alegre M, Yapa MT, de Villiers A, Sandra P, et al. Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts. JOURNAL OF SEPARATION SCIENCE. 2015;38(5):724–31.
IEEE
[1]
S. De Smet et al., “Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts,” JOURNAL OF SEPARATION SCIENCE, vol. 38, no. 5, pp. 724–731, 2015.
@article{5845680,
  abstract     = {{This paper reports the optimization of the online coupling of 2,2’-azino-bis(3-ethylbenzothiazoline)-6 sulfonic acid based radical scavenging assays with reversed  phase HPLC. The residence times in the reactor was reduced down to 6.4 seconds to ensure minimal peak broadening and loss of separation. Peak capacity losses between compound detection and measurement of the radical scavenging potential were reduced to 10% and lower on coupled column systems. The methodology was successfully applied for the detection of the scavenging activity of molecules encompassing a broad hydrophobicity range. The method shows promise for the assessment of low molecular weight polyphenols in red wine via coupled column high resolution HPLC-MS analysis.}},
  author       = {{De Smet, Seppe and Miserez, Bram and Rambla Alegre, Maria and Yapa, Mehmet Talha and de Villiers, André and Sandra, Patrick and Lynen, Frederic}},
  issn         = {{1615-9306}},
  journal      = {{JOURNAL OF SEPARATION SCIENCE}},
  keywords     = {{Wine,PHENOLIC-COMPOUNDS,Radicals,High-performance liquid chromatography,Antioxidants,HPLC,IDENTIFICATION,CAPACITY,CATION,WINES,CHEMILUMINESCENCE,POLYPHENOLS,METABOLITES,CHEMISTRY}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{724--731}},
  title        = {{Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts}},
  url          = {{http://doi.org/10.1002/jssc.201401222}},
  volume       = {{38}},
  year         = {{2015}},
}
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