Advanced search
1 file | 4.55 MB

Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways

(2014) PLOS ONE. 9(12).
Author
Organization
Project
Bioinformatics: from nucleotids to networks (N2N)
Abstract
Sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. Other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. Here we aim to study the effects of the association between the diazotroph Gluconacetobacter diazotrophicus PAL5 and sugarcane cv. SP70-1143 during water depletion by characterizing differential transcriptome profiles of sugarcane. RNA-seq libraries were generated from roots and shoots of sugarcane plants free of endophytes that were inoculated with G. diazotrophicus and subjected to water depletion for 3 days. A sugarcane reference transcriptome was constructed and used for the identification of differentially expressed transcripts. The differential profile of non-inoculated SP70-1143 suggests that it responds to water deficit stress by the activation of drought-responsive markers and hormone pathways, as ABA and Ethylene. qRT-PCR revealed that root samples had higher levels of G. diazotrophicus 3 days after water deficit, compared to roots of inoculated plants watered normally. With prolonged drought only inoculated plants survived, indicating that SP70-1143 plants colonized with G. diazotrophicus become more tolerant to drought stress than non-inoculated plants. Strengthening this hypothesis, several gene expression responses to drought were inactivated or regulated in an opposite manner, especially in roots, when plants were colonized by the bacteria. The data suggests that colonized roots would not be suffering from stress in the same way as non-inoculated plants. On the other hand, shoots specifically activate ABA-dependent signaling genes, which could act as key elements in the drought resistance conferred by G. diazotrophicus to SP70-1143. This work reports for the first time the involvement of G. diazotrophicus in the promotion of drought-tolerance to sugarcane cv. SP70-1143, and it describes the initial molecular events that may trigger the increased drought tolerance in the host plant.
Keywords
ABSCISIC-ACID, ABIOTIC STRESS RESPONSES, GENE-EXPRESSION, NITROGEN-FIXATION, RNA-SEQ, ARABIDOPSIS-THALIANA, SIGNALING PATHWAYS, STOMATAL APERTURE, WATER-STRESS, PLANT-RESPONSES

Downloads

  • Vargas et al. 2014 PLoS ONE 9 e114744.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 4.55 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Vargas, Lívia, Ailton B Santa Brigida, José P Mota Filho, Thais G de Carvalho, Cristian A Rojas, Dries Vaneechoutte, Michiel Van Bel, et al. 2014. “Drought Tolerance Conferred to Sugarcane by Association with Gluconacetobacter Diazotrophicus: a Transcriptomic View of Hormone Pathways.” Plos One 9 (12).
APA
Vargas, L., Santa Brigida, A. B., Mota Filho, J. P., de Carvalho, T. G., Rojas, C. A., Vaneechoutte, D., Van Bel, M., et al. (2014). Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways. PLOS ONE, 9(12).
Vancouver
1.
Vargas L, Santa Brigida AB, Mota Filho JP, de Carvalho TG, Rojas CA, Vaneechoutte D, et al. Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways. PLOS ONE. 2014;9(12).
MLA
Vargas, Lívia, Ailton B Santa Brigida, José P Mota Filho, et al. “Drought Tolerance Conferred to Sugarcane by Association with Gluconacetobacter Diazotrophicus: a Transcriptomic View of Hormone Pathways.” PLOS ONE 9.12 (2014): n. pag. Print.
@article{5841790,
  abstract     = {Sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. Other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. Here we aim to study the effects of the association between the diazotroph Gluconacetobacter diazotrophicus PAL5 and sugarcane cv. SP70-1143 during water depletion by characterizing differential transcriptome profiles of sugarcane. RNA-seq libraries were generated from roots and shoots of sugarcane plants free of endophytes that were inoculated with G. diazotrophicus and subjected to water depletion for 3 days. A sugarcane reference transcriptome was constructed and used for the identification of differentially expressed transcripts. The differential profile of non-inoculated SP70-1143 suggests that it responds to water deficit stress by the activation of drought-responsive markers and hormone pathways, as ABA and Ethylene. qRT-PCR revealed that root samples had higher levels of G. diazotrophicus 3 days after water deficit, compared to roots of inoculated plants watered normally. With prolonged drought only inoculated plants survived, indicating that SP70-1143 plants colonized with G. diazotrophicus become more tolerant to drought stress than non-inoculated plants. Strengthening this hypothesis, several gene expression responses to drought were inactivated or regulated in an opposite manner, especially in roots, when plants were colonized by the bacteria. The data suggests that colonized roots would not be suffering from stress in the same way as non-inoculated plants. On the other hand, shoots specifically activate ABA-dependent signaling genes, which could act as key elements in the drought resistance conferred by G. diazotrophicus to SP70-1143. This work reports for the first time the involvement of G. diazotrophicus in the promotion of drought-tolerance to sugarcane cv. SP70-1143, and it describes the initial molecular events that may trigger the increased drought tolerance in the host plant.},
  articleno    = {e114744},
  author       = {Vargas, L{\'i}via and Santa Brigida, Ailton B and Mota Filho, Jos{\'e} P and de Carvalho, Thais G and Rojas, Cristian A and Vaneechoutte, Dries and Van Bel, Michiel and Farrinelli, Laurent and Ferreira, Paulo CG and Vandepoele, Klaas and Hemerly, Adriana S},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {ABSCISIC-ACID,ABIOTIC STRESS RESPONSES,GENE-EXPRESSION,NITROGEN-FIXATION,RNA-SEQ,ARABIDOPSIS-THALIANA,SIGNALING PATHWAYS,STOMATAL APERTURE,WATER-STRESS,PLANT-RESPONSES},
  language     = {eng},
  number       = {12},
  pages        = {37},
  title        = {Drought tolerance conferred to sugarcane by association with Gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways},
  url          = {http://dx.doi.org/10.1371/journal.pone.0114744},
  volume       = {9},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: