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Gene expression profiling of giant cell tumor of bone reveals downregulation of extracellular matrix components decorin and lumican associated with lung metastasis

(2014) VIRCHOWS ARCHIV. 465(6). p.703-713
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Organization
Abstract
Giant cell tumor of bone (GCTB) displays worrisome clinical features such as local recurrence and occasionally metastatic disease which are unpredictable by morphology. Additional routinely usable biomarkers do not exist. Gene expression profiles of six clinically defined groups of GCTB and one group of aneurysmal bone cyst (ABC) were determined by microarray (n = 33). The most promising differentially expressed genes were validated by Q-PCR as potential biomarkers in a larger patient group (n = 41). Corresponding protein expression was confirmed by immunohistochemistry. Unsupervised hierarchical clustering reveals a metastatic GCTB cluster, a heterogeneous, non-metastatic GCTB cluster, and a primary ABC cluster. Balanced score testing indicates that lumican (LUM) and decorin (DCN) are the most promising biomarkers as they have lower level of expression in the metastatic group. Expression of dermatopontin (DPT) was significantly lower in recurrent tumors. Validation of the results was performed by paired and unpaired t test in primary GCTB and corresponding metastases, which proved that the differential expression of LUM and DCN is tumor specific rather than location specific. Our findings show that several genes related to extracellular matrix integrity (LUM, DCN, and DPT) are differentially expressed and may serve as biomarkers for metastatic and recurrent GCTB.
Keywords
Decorin, Giant cell tumor of bone, Osteoclastogenesis, Gene expression profiling, Extracellular matrix, GROWTH-FACTOR-BETA, LEUCINE-RICH PROTEOGLYCANS, OSTEOCLAST FORMATION, SKIN FRAGILITY, RECEPTOR, DERMATOPONTIN, LIGAND, VEGF, DIFFERENTIATION, PROGRESSION

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MLA
Lieveld, Marusya, ERIK BODSON, G De Boeck, et al. “Gene Expression Profiling of Giant Cell Tumor of Bone Reveals Downregulation of Extracellular Matrix Components Decorin and Lumican Associated with Lung Metastasis.” VIRCHOWS ARCHIV 465.6 (2014): 703–713. Print.
APA
Lieveld, Marusya, BODSON, E., De Boeck, G., NOUMAN, B., Cleton-Jansen, A., Korsching, E., Benassi, M., et al. (2014). Gene expression profiling of giant cell tumor of bone reveals downregulation of extracellular matrix components decorin and lumican associated with lung metastasis. VIRCHOWS ARCHIV, 465(6), 703–713.
Chicago author-date
Lieveld, Marusya, ERIK BODSON, G De Boeck, BOUCHRA NOUMAN, AM Cleton-Jansen, E Korsching, MS Benassi, et al. 2014. “Gene Expression Profiling of Giant Cell Tumor of Bone Reveals Downregulation of Extracellular Matrix Components Decorin and Lumican Associated with Lung Metastasis.” Virchows Archiv 465 (6): 703–713.
Chicago author-date (all authors)
Lieveld, Marusya, ERIK BODSON, G De Boeck, BOUCHRA NOUMAN, AM Cleton-Jansen, E Korsching, MS Benassi, P Picci, Gwen Sys, Bart Poffyn, NA Athanasou, PCW Hogendoorn, and Ramses Forsyth. 2014. “Gene Expression Profiling of Giant Cell Tumor of Bone Reveals Downregulation of Extracellular Matrix Components Decorin and Lumican Associated with Lung Metastasis.” Virchows Archiv 465 (6): 703–713.
Vancouver
1.
Lieveld M, BODSON E, De Boeck G, NOUMAN B, Cleton-Jansen A, Korsching E, et al. Gene expression profiling of giant cell tumor of bone reveals downregulation of extracellular matrix components decorin and lumican associated with lung metastasis. VIRCHOWS ARCHIV. 2014;465(6):703–13.
IEEE
[1]
M. Lieveld et al., “Gene expression profiling of giant cell tumor of bone reveals downregulation of extracellular matrix components decorin and lumican associated with lung metastasis,” VIRCHOWS ARCHIV, vol. 465, no. 6, pp. 703–713, 2014.
@article{5838186,
  abstract     = {Giant cell tumor of bone (GCTB) displays worrisome clinical features such as local recurrence and occasionally metastatic disease which are unpredictable by morphology. Additional routinely usable biomarkers do not exist. Gene expression profiles of six clinically defined groups of GCTB and one group of aneurysmal bone cyst (ABC) were determined by microarray (n = 33). The most promising differentially expressed genes were validated by Q-PCR as potential biomarkers in a larger patient group (n = 41). Corresponding protein expression was confirmed by immunohistochemistry. Unsupervised hierarchical clustering reveals a metastatic GCTB cluster, a heterogeneous, non-metastatic GCTB cluster, and a primary ABC cluster. Balanced score testing indicates that lumican (LUM) and decorin (DCN) are the most promising biomarkers as they have lower level of expression in the metastatic group. Expression of dermatopontin (DPT) was significantly lower in recurrent tumors. Validation of the results was performed by paired and unpaired t test in primary GCTB and corresponding metastases, which proved that the differential expression of LUM and DCN is tumor specific rather than location specific. Our findings show that several genes related to extracellular matrix integrity (LUM, DCN, and DPT) are differentially expressed and may serve as biomarkers for metastatic and recurrent GCTB.},
  author       = {Lieveld, Marusya and BODSON, ERIK and De Boeck, G and NOUMAN, BOUCHRA and Cleton-Jansen, AM and Korsching, E and Benassi, MS and Picci, P and Sys, Gwen and Poffyn, Bart and Athanasou, NA and Hogendoorn, PCW and Forsyth, Ramses},
  issn         = {0945-6317},
  journal      = {VIRCHOWS ARCHIV},
  keywords     = {Decorin,Giant cell tumor of bone,Osteoclastogenesis,Gene expression profiling,Extracellular matrix,GROWTH-FACTOR-BETA,LEUCINE-RICH PROTEOGLYCANS,OSTEOCLAST FORMATION,SKIN FRAGILITY,RECEPTOR,DERMATOPONTIN,LIGAND,VEGF,DIFFERENTIATION,PROGRESSION},
  language     = {eng},
  number       = {6},
  pages        = {703--713},
  title        = {Gene expression profiling of giant cell tumor of bone reveals downregulation of extracellular matrix components decorin and lumican associated with lung metastasis},
  url          = {http://dx.doi.org/10.1007/s00428-014-1666-7},
  volume       = {465},
  year         = {2014},
}

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