Advanced search
1 file | 1.42 MB Add to list

The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes

Author
Organization
Abstract
In the brain, astrocytes represent the cellular population that expresses the highest amount of connexins (Cxs). This family of membrane proteins is the molecular constituent of gap junction channels and hemichannels that provide pathways for direct cytoplasm-to-cytoplasm and inside-out exchange, respectively. Both types of Cx channels are permeable to ions and small signaling molecules allowing astrocytes to establish dynamic interactions with neurons. So far, most pharmacological approaches currently available do not distinguish between these two channel functions, stressing the need to develop new specific molecular tools. In astrocytes two major Cxs are expressed, Cx43 and Cx30, and there is now evidence indicating that at least Cx43 operates as a gap junction channel as well as a hemichannel in these cells. Based on studies in primary cultures as well as in acute hippocampal slices, we report here that Gap 19, a nonapeptide derived from the cytoplasmic loop of Cx43, inhibits astroglial Cx43 hemichannels in a dose-dependent manner, without affecting gap junction channels. This peptide, which not only selectively inhibits hemichannels but is also specific for Cx43, can be delivered in vivo in mice as TAT-Gap19, and displays penetration into the brain parenchyma. As a result, Gap 19 combined with other tools opens up new avenues to decipher the role of Cx43 hemichannels in interactions between astrocytes and neurons in physiological as well as pathological situations.
Keywords
glial cells, connexins, gap junctions, astroglia, mimetic peptide, BLOOD-BRAIN-BARRIER, ASTROGLIAL NETWORKS, INTERCELLULAR COMMUNICATION, LOOP/TAIL INTERACTIONS, SYNAPTIC-TRANSMISSION, CULTURED ASTROCYTES, CX43 HEMICHANNELS, CELL-DEATH, CHANNELS, RELEASE

Downloads

  • Abudara2014.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.42 MB

Citation

Please use this url to cite or link to this publication:

MLA
Abudara, Verónica, et al. “The Connexin43 Mimetic Peptide Gap19 Inhibits Hemichannels without Altering Gap Junctional Communication in Astrocytes.” FRONTIERS IN CELLULAR NEUROSCIENCE, vol. 8, 2014, doi:10.3389/fncel.2014.00306.
APA
Abudara, V., Bechberger, J., Freitas-Andrade, M., De Bock, M., Wang, N., Bultynck, G., … Giaume, C. (2014). The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes. FRONTIERS IN CELLULAR NEUROSCIENCE, 8. https://doi.org/10.3389/fncel.2014.00306
Chicago author-date
Abudara, Verónica, John Bechberger, Moises Freitas-Andrade, Marijke De Bock, Nan Wang, Geert Bultynck, Christian C Naus, Luc Leybaert, and Christian Giaume. 2014. “The Connexin43 Mimetic Peptide Gap19 Inhibits Hemichannels without Altering Gap Junctional Communication in Astrocytes.” FRONTIERS IN CELLULAR NEUROSCIENCE 8. https://doi.org/10.3389/fncel.2014.00306.
Chicago author-date (all authors)
Abudara, Verónica, John Bechberger, Moises Freitas-Andrade, Marijke De Bock, Nan Wang, Geert Bultynck, Christian C Naus, Luc Leybaert, and Christian Giaume. 2014. “The Connexin43 Mimetic Peptide Gap19 Inhibits Hemichannels without Altering Gap Junctional Communication in Astrocytes.” FRONTIERS IN CELLULAR NEUROSCIENCE 8. doi:10.3389/fncel.2014.00306.
Vancouver
1.
Abudara V, Bechberger J, Freitas-Andrade M, De Bock M, Wang N, Bultynck G, et al. The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes. FRONTIERS IN CELLULAR NEUROSCIENCE. 2014;8.
IEEE
[1]
V. Abudara et al., “The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes,” FRONTIERS IN CELLULAR NEUROSCIENCE, vol. 8, 2014.
@article{5829802,
  abstract     = {{In the brain, astrocytes represent the cellular population that expresses the highest amount of connexins (Cxs). This family of membrane proteins is the molecular constituent of gap junction channels and hemichannels that provide pathways for direct cytoplasm-to-cytoplasm and inside-out exchange, respectively. Both types of Cx channels are permeable to ions and small signaling molecules allowing astrocytes to establish dynamic interactions with neurons. So far, most pharmacological approaches currently available do not distinguish between these two channel functions, stressing the need to develop new specific molecular tools. In astrocytes two major Cxs are expressed, Cx43 and Cx30, and there is now evidence indicating that at least Cx43 operates as a gap junction channel as well as a hemichannel in these cells. Based on studies in primary cultures as well as in acute hippocampal slices, we report here that Gap 19, a nonapeptide derived from the cytoplasmic loop of Cx43, inhibits astroglial Cx43 hemichannels in a dose-dependent manner, without affecting gap junction channels. This peptide, which not only selectively inhibits hemichannels but is also specific for Cx43, can be delivered in vivo in mice as TAT-Gap19, and displays penetration into the brain parenchyma. As a result, Gap 19 combined with other tools opens up new avenues to decipher the role of Cx43 hemichannels in interactions between astrocytes and neurons in physiological as well as pathological situations.}},
  articleno    = {{306}},
  author       = {{Abudara, Verónica and Bechberger, John and Freitas-Andrade, Moises and De Bock, Marijke and Wang, Nan and Bultynck, Geert and Naus, Christian C and Leybaert, Luc and Giaume, Christian}},
  issn         = {{1662-5102}},
  journal      = {{FRONTIERS IN CELLULAR NEUROSCIENCE}},
  keywords     = {{glial cells,connexins,gap junctions,astroglia,mimetic peptide,BLOOD-BRAIN-BARRIER,ASTROGLIAL NETWORKS,INTERCELLULAR COMMUNICATION,LOOP/TAIL INTERACTIONS,SYNAPTIC-TRANSMISSION,CULTURED ASTROCYTES,CX43 HEMICHANNELS,CELL-DEATH,CHANNELS,RELEASE}},
  language     = {{eng}},
  pages        = {{8}},
  title        = {{The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes}},
  url          = {{http://doi.org/10.3389/fncel.2014.00306}},
  volume       = {{8}},
  year         = {{2014}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: