Advanced search
1 file | 427.67 KB Add to list

Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus

Author
Organization
Abstract
The effects of different calcium (Ca2+), magnesium (Mg2+), and zinc (Zn2+) concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus was studied. The culture system consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl, 1.011 g/L glucose, 1,000 UI/ml penicillin, 1,000 mu g/ml treptomycin, heat inactivated fetal calf serum (FCS) 20% for primary cell culture and 10% for subculture. The RNA/DNA ratio of the cell cultures was measured. The results show that the cell division of hepatopancreatic cells of P. japonicus was enhanced by the optimal concentration of inorganic salt (Ca2+, 1.0 g/L; Mg2+, 5.0 g/L; Zn2+, 80 mu g/L). The hepatopancreatic cell culture system and improved culture conditions described here will be very useful for in vitro experiments to study viruses responsible for infections in shrimp leading to tremendous economic losses.
Keywords
LYMPHOID ORGAN, CULTURED-CELLS, SHRIMP, GROWTH, VIRUS, INFECTION, BACULOVIRUS MBV, ENDOTHELIAL-CELLS, RNA-DNA RATIO, ELECTRON-MICROSCOPIC EVIDENCE, Zn2+, Mg2+, Ca2+, Cell culture, Penaeus japonicus, Hepatopancreatic

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 427.67 KB

Citation

Please use this url to cite or link to this publication:

MLA
Wang, Hong-Wei, Duanbo Cai, Kaiming Li, et al. “Effects of Different Calcium, Magnesium, and Zinc Concentrations Supplemented on Hepatopancreatic Cell Proliferation of Kuruma Prawn, Penaeus Japonicus.” BIOLOGICAL TRACE ELEMENT RESEARCH 148.2 (2012): 198–202. Print.
APA
Wang, H.-W., Cai, D., Li, K., Xu, H., Yang, L., & Zhu, N. (2012). Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus. BIOLOGICAL TRACE ELEMENT RESEARCH, 148(2), 198–202.
Chicago author-date
Wang, Hong-Wei, Duanbo Cai, Kaiming Li, Haiming Xu, Likun Yang, and Na Zhu. 2012. “Effects of Different Calcium, Magnesium, and Zinc Concentrations Supplemented on Hepatopancreatic Cell Proliferation of Kuruma Prawn, Penaeus Japonicus.” Biological Trace Element Research 148 (2): 198–202.
Chicago author-date (all authors)
Wang, Hong-Wei, Duanbo Cai, Kaiming Li, Haiming Xu, Likun Yang, and Na Zhu. 2012. “Effects of Different Calcium, Magnesium, and Zinc Concentrations Supplemented on Hepatopancreatic Cell Proliferation of Kuruma Prawn, Penaeus Japonicus.” Biological Trace Element Research 148 (2): 198–202.
Vancouver
1.
Wang H-W, Cai D, Li K, Xu H, Yang L, Zhu N. Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus. BIOLOGICAL TRACE ELEMENT RESEARCH. 2012;148(2):198–202.
IEEE
[1]
H.-W. Wang, D. Cai, K. Li, H. Xu, L. Yang, and N. Zhu, “Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus,” BIOLOGICAL TRACE ELEMENT RESEARCH, vol. 148, no. 2, pp. 198–202, 2012.
@article{5827475,
  abstract     = {The effects of different calcium (Ca2+), magnesium (Mg2+), and zinc (Zn2+) concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus was studied. The culture system consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl, 1.011 g/L glucose, 1,000 UI/ml penicillin, 1,000 mu g/ml treptomycin, heat inactivated fetal calf serum (FCS) 20% for primary cell culture and 10% for subculture. The RNA/DNA ratio of the cell cultures was measured. The results show that the cell division of hepatopancreatic cells of P. japonicus was enhanced by the optimal concentration of inorganic salt (Ca2+, 1.0 g/L; Mg2+, 5.0 g/L; Zn2+, 80 mu g/L). The hepatopancreatic cell culture system and improved culture conditions described here will be very useful for in vitro experiments to study viruses responsible for infections in shrimp leading to tremendous economic losses.},
  author       = {Wang, Hong-Wei and Cai, Duanbo and Li, Kaiming and Xu, Haiming and Yang, Likun and Zhu, Na},
  issn         = {0163-4984},
  journal      = {BIOLOGICAL TRACE ELEMENT RESEARCH},
  keywords     = {LYMPHOID ORGAN,CULTURED-CELLS,SHRIMP,GROWTH,VIRUS,INFECTION,BACULOVIRUS MBV,ENDOTHELIAL-CELLS,RNA-DNA RATIO,ELECTRON-MICROSCOPIC EVIDENCE,Zn2+,Mg2+,Ca2+,Cell culture,Penaeus japonicus,Hepatopancreatic},
  language     = {eng},
  number       = {2},
  pages        = {198--202},
  title        = {Effects of different calcium, magnesium, and zinc concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus},
  url          = {http://dx.doi.org/10.1007/s12011-012-9366-0},
  volume       = {148},
  year         = {2012},
}

Altmetric
View in Altmetric
Web of Science
Times cited: