- Author
- Mieke Boone (UGent) , Maxime Willems (UGent) , Stijn Mouton (UGent) , Tom Artois and Gaetan Borgonie (UGent)
- Organization
- Abstract
- Monitoring the embryonic development is of fundamental importance for developmental biology. Here we report a successful experimental protocol for immunohistological and immunocytological localization of developmental characters in whole-mount embryos of Macrostomum lignano. Conventional techniques for immunolocalization in flatworm embryos engender the use of sharpened needles or a micro-injection system to punctuate the impermeable eggshell. However, these methods are either time-consuming or requiring a lot of dexterity (puncturing) or expensive (micro-injection system). We illustrate the advantages of this new technique by several examples of immunolocalization in M. lignano and discuss the optimal conditions for the protocol in M. lignano.
- Keywords
- embryonic stem cells, development, protocol, Macrostomum, Turbellaria
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-582624
- MLA
- Boone, Mieke, et al. Freeze Cracking Protocol for Permiabilization of Macrostomum Lignano Eggs. 2007.
- APA
- Boone, M., Willems, M., Mouton, S., Artois, T., & Borgonie, G. (2007). Freeze cracking protocol for permiabilization of Macrostomum lignano eggs. Presented at the 1st International Macrostomum Meeting, Basel, Switserland.
- Chicago author-date
- Boone, Mieke, Maxime Willems, Stijn Mouton, Tom Artois, and Gaetan Borgonie. 2007. “Freeze Cracking Protocol for Permiabilization of Macrostomum Lignano Eggs.” In .
- Chicago author-date (all authors)
- Boone, Mieke, Maxime Willems, Stijn Mouton, Tom Artois, and Gaetan Borgonie. 2007. “Freeze Cracking Protocol for Permiabilization of Macrostomum Lignano Eggs.” In .
- Vancouver
- 1.Boone M, Willems M, Mouton S, Artois T, Borgonie G. Freeze cracking protocol for permiabilization of Macrostomum lignano eggs. In 2007.
- IEEE
- [1]M. Boone, M. Willems, S. Mouton, T. Artois, and G. Borgonie, “Freeze cracking protocol for permiabilization of Macrostomum lignano eggs,” presented at the 1st International Macrostomum Meeting, Basel, Switserland, 2007.
@inproceedings{582624, abstract = {{Monitoring the embryonic development is of fundamental importance for developmental biology. Here we report a successful experimental protocol for immunohistological and immunocytological localization of developmental characters in whole-mount embryos of Macrostomum lignano. Conventional techniques for immunolocalization in flatworm embryos engender the use of sharpened needles or a micro-injection system to punctuate the impermeable eggshell. However, these methods are either time-consuming or requiring a lot of dexterity (puncturing) or expensive (micro-injection system). We illustrate the advantages of this new technique by several examples of immunolocalization in M. lignano and discuss the optimal conditions for the protocol in M. lignano.}}, author = {{Boone, Mieke and Willems, Maxime and Mouton, Stijn and Artois, Tom and Borgonie, Gaetan}}, keywords = {{embryonic stem cells,development,protocol,Macrostomum,Turbellaria}}, language = {{eng}}, location = {{Basel, Switserland}}, title = {{Freeze cracking protocol for permiabilization of Macrostomum lignano eggs}}, year = {{2007}}, }