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Freeze cracking protocol for permiabilization of Macrostomum lignano eggs

Mieke Boone (UGent) , Maxime Willems (UGent) , Stijn Mouton (UGent) , Tom Artois and Gaetan Borgonie (UGent)
(2007)
Author
Organization
Abstract
Monitoring the embryonic development is of fundamental importance for developmental biology. Here we report a successful experimental protocol for immunohistological and immunocytological localization of developmental characters in whole-mount embryos of Macrostomum lignano. Conventional techniques for immunolocalization in flatworm embryos engender the use of sharpened needles or a micro-injection system to punctuate the impermeable eggshell. However, these methods are either time-consuming or requiring a lot of dexterity (puncturing) or expensive (micro-injection system). We illustrate the advantages of this new technique by several examples of immunolocalization in M. lignano and discuss the optimal conditions for the protocol in M. lignano.
Keywords
embryonic stem cells, development, protocol, Macrostomum, Turbellaria

Citation

Please use this url to cite or link to this publication:

MLA
Boone, Mieke, et al. Freeze Cracking Protocol for Permiabilization of Macrostomum Lignano Eggs. 2007.
APA
Boone, M., Willems, M., Mouton, S., Artois, T., & Borgonie, G. (2007). Freeze cracking protocol for permiabilization of Macrostomum lignano eggs. Presented at the 1st International Macrostomum Meeting, Basel, Switserland.
Chicago author-date
Boone, Mieke, Maxime Willems, Stijn Mouton, Tom Artois, and Gaetan Borgonie. 2007. “Freeze Cracking Protocol for Permiabilization of Macrostomum Lignano Eggs.” In .
Chicago author-date (all authors)
Boone, Mieke, Maxime Willems, Stijn Mouton, Tom Artois, and Gaetan Borgonie. 2007. “Freeze Cracking Protocol for Permiabilization of Macrostomum Lignano Eggs.” In .
Vancouver
1.
Boone M, Willems M, Mouton S, Artois T, Borgonie G. Freeze cracking protocol for permiabilization of Macrostomum lignano eggs. In 2007.
IEEE
[1]
M. Boone, M. Willems, S. Mouton, T. Artois, and G. Borgonie, “Freeze cracking protocol for permiabilization of Macrostomum lignano eggs,” presented at the 1st International Macrostomum Meeting, Basel, Switserland, 2007.
@inproceedings{582624,
  abstract     = {{Monitoring the embryonic development is of fundamental importance for developmental biology. Here we report a successful experimental protocol for immunohistological and immunocytological localization of developmental characters in whole-mount embryos of Macrostomum lignano. Conventional techniques for immunolocalization in flatworm embryos engender the use of sharpened needles or a micro-injection system to punctuate the impermeable eggshell. However, these methods are either time-consuming or requiring a lot of dexterity (puncturing) or expensive (micro-injection system). We illustrate the advantages of this new technique by several examples of immunolocalization in M. lignano and discuss the optimal conditions for the protocol in M. lignano.}},
  author       = {{Boone, Mieke and Willems, Maxime and Mouton, Stijn and Artois, Tom and Borgonie, Gaetan}},
  keywords     = {{embryonic stem cells,development,protocol,Macrostomum,Turbellaria}},
  language     = {{eng}},
  location     = {{Basel, Switserland}},
  title        = {{Freeze cracking protocol for permiabilization of Macrostomum lignano eggs}},
  year         = {{2007}},
}