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Dynamic changes of the ethylene biosynthesis in 'Jonagold' apple

(2014) PHYSIOLOGIA PLANTARUM. 150(2). p.161-173
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Abstract
In this study, the short-term and dynamic changes of the ethylene biosynthesis of Jonagold apple during and after application of controlled atmosphere (CA) storage conditions were quantified using a systems biology approach. Rapid responses to imposed temperature and atmospheric conditions were captured by continuous online photoacoustic ethylene measurements. Discrete destructive sampling was done to understand observed changes of ethylene biosynthesis at the transcriptional, translational and metabolic level. Application of the ethylene inhibitor 1-methylcyclopropene (1-MCP) allowed for the discrimination between ethylene-mediated changes and ethylene-independent changes related to the imposed conditions. Online ethylene measurements showed fast and slower responses during and after application of CA conditions. The changes in 1-aminocyclopropane-1-carboxylate synthase (ACS) activity were most correlated with changes in ACS1 expression and regulated the cold-induced increase in ethylene production during the early chilling phase. Transcription of ACS3 was found ethylene independent and was triggered upon warming of CA-stored apples. Increased expression of ACO1 during shelf life led to a strong increase in 1-aminocyclopropane-1-carboxylate oxidase (ACO) activity, required for the exponential production of ethylene during system 2. Expression of ACO2 and ACO3 was upregulated in 1-MCP-treated fruit showing a negative correlation with ethylene production. ACO activity never became rate limiting.
Keywords
1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID, PROTEIN, GRANNY-SMITH APPLES, MDACS3 GENE, FRUIT, METHIONINE, IDENTIFICATION, INTERMEDIATE, METABOLISM, PERCEPTION

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MLA
Bulens, Inge, Bram Van de Poel, Maarten LATM Hertog, et al. “Dynamic Changes of the Ethylene Biosynthesis in ‘Jonagold’ Apple.” PHYSIOLOGIA PLANTARUM 150.2 (2014): 161–173. Print.
APA
Bulens, Inge, Van de Poel, B., Hertog, M. L., Cristescu, S. M., Harren, F. J., De Proft, M. P., Geeraerd, A. H., et al. (2014). Dynamic changes of the ethylene biosynthesis in “Jonagold” apple. PHYSIOLOGIA PLANTARUM, 150(2), 161–173.
Chicago author-date
Bulens, Inge, Bram Van de Poel, Maarten LATM Hertog, Simona M Cristescu, Frans JM Harren, Maurice P De Proft, Annemie H Geeraerd, and Bart M Nicolaï. 2014. “Dynamic Changes of the Ethylene Biosynthesis in ‘Jonagold’ Apple.” Physiologia Plantarum 150 (2): 161–173.
Chicago author-date (all authors)
Bulens, Inge, Bram Van de Poel, Maarten LATM Hertog, Simona M Cristescu, Frans JM Harren, Maurice P De Proft, Annemie H Geeraerd, and Bart M Nicolaï. 2014. “Dynamic Changes of the Ethylene Biosynthesis in ‘Jonagold’ Apple.” Physiologia Plantarum 150 (2): 161–173.
Vancouver
1.
Bulens I, Van de Poel B, Hertog ML, Cristescu SM, Harren FJ, De Proft MP, et al. Dynamic changes of the ethylene biosynthesis in “Jonagold” apple. PHYSIOLOGIA PLANTARUM. 2014;150(2):161–73.
IEEE
[1]
I. Bulens et al., “Dynamic changes of the ethylene biosynthesis in ‘Jonagold’ apple,” PHYSIOLOGIA PLANTARUM, vol. 150, no. 2, pp. 161–173, 2014.
@article{5820208,
  abstract     = {In this study, the short-term and dynamic changes of the ethylene biosynthesis of Jonagold apple during and after application of controlled atmosphere (CA) storage conditions were quantified using a systems biology approach. Rapid responses to imposed temperature and atmospheric conditions were captured by continuous online photoacoustic ethylene measurements. Discrete destructive sampling was done to understand observed changes of ethylene biosynthesis at the transcriptional, translational and metabolic level. Application of the ethylene inhibitor 1-methylcyclopropene (1-MCP) allowed for the discrimination between ethylene-mediated changes and ethylene-independent changes related to the imposed conditions. Online ethylene measurements showed fast and slower responses during and after application of CA conditions. The changes in 1-aminocyclopropane-1-carboxylate synthase (ACS) activity were most correlated with changes in ACS1 expression and regulated the cold-induced increase in ethylene production during the early chilling phase. Transcription of ACS3 was found ethylene independent and was triggered upon warming of CA-stored apples. Increased expression of ACO1 during shelf life led to a strong increase in 1-aminocyclopropane-1-carboxylate oxidase (ACO) activity, required for the exponential production of ethylene during system 2. Expression of ACO2 and ACO3 was upregulated in 1-MCP-treated fruit showing a negative correlation with ethylene production. ACO activity never became rate limiting.},
  author       = {Bulens, Inge and Van de Poel, Bram and Hertog, Maarten LATM and Cristescu, Simona M and Harren, Frans JM and De Proft, Maurice P and Geeraerd, Annemie H and Nicolaï, Bart M},
  issn         = {0031-9317},
  journal      = {PHYSIOLOGIA PLANTARUM},
  keywords     = {1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID,PROTEIN,GRANNY-SMITH APPLES,MDACS3 GENE,FRUIT,METHIONINE,IDENTIFICATION,INTERMEDIATE,METABOLISM,PERCEPTION},
  language     = {eng},
  number       = {2},
  pages        = {161--173},
  title        = {Dynamic changes of the ethylene biosynthesis in 'Jonagold' apple},
  url          = {http://dx.doi.org/10.1111/ppl.12084},
  volume       = {150},
  year         = {2014},
}

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