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RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions

Yann Estornes (UGent) , Miguel Aguileta (UGent) , Christel Dubuisson (UGent) , Julie De Keyser (UGent) , Vera Goossens (UGent) , Kristof Kersse (UGent) , A Samali, Peter Vandenabeele (UGent) and Mathieu Bertrand (UGent)
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Ghent researchers on unfolded proteins in inflammatory disease (GROUP-ID)
Abstract
Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress and results in the activation of the unfolded protein response (UPR), which aims at restoring ER homeostasis. However, when the stress is too severe the UPR switches from being a pro-survival response to a pro-death one, and the molecular mechanisms underlying ER stress-mediated death have remained incompletely understood. In this study, we identified receptor interacting protein kinase 1 (RIPK1)-a kinase at the crossroad between life and death downstream of various receptors-as a new regulator of ER stress-induced death. We found that Ripk1-deficient MEFs are protected from apoptosis induced by ER stressors, which is reflected by reduced caspase activation and PARP processing. Interestingly, the pro-apoptotic role of Ripk1 is independent of its kinase activity, is not regulated by its cIAP1/2-mediated ubiquitylation, and does not rely on the direct regulation of JNK or CHOP, two reportedly main players in ER stress-induced death. Instead, we found that ER stress-induced apoptosis in these cells relies on death receptor-independent activation of caspase-8, and identified Ripk1 upstream of caspase-8. However, in contrast to RIPK1-dependent apoptosis downstream of TNFR1, we did not find Ripk1 associated with caspase-8 in a death-inducing complex upon unresolved ER stress. Our data rather suggest that RIPK1 indirectly regulates caspase-8 activation, in part via interaction with the ER stress sensor inositol-requiring protein 1 (IRE1).
Keywords
ENDOPLASMIC-RETICULUM STRESS, UNFOLDED-PROTEIN-RESPONSE, NF-KAPPA-B, ALPHA-DEPENDENT APOPTOSIS, CYTOCHROME-C RELEASE, CELL-DEATH, DOWN-REGULATION, MESSENGER-RNAS, UP-REGULATION, ACTIVATION

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Citation

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Chicago
Estornes, Yann, Miguel Aguileta, Christel Dubuisson, Julie De Keyser, Vera Goossens, Kristof Kersse, A Samali, Peter Vandenabeele, and Mathieu Bertrand. 2014. “RIPK1 Promotes Death Receptor-independent Caspase-8-mediated Apoptosis Under Unresolved ER Stress Conditions.” Cell Death & Disease 5.
APA
Estornes, Y., Aguileta, M., Dubuisson, C., De Keyser, J., Goossens, V., Kersse, K., Samali, A., et al. (2014). RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions. CELL DEATH & DISEASE, 5.
Vancouver
1.
Estornes Y, Aguileta M, Dubuisson C, De Keyser J, Goossens V, Kersse K, et al. RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions. CELL DEATH & DISEASE. 2014;5.
MLA
Estornes, Yann, Miguel Aguileta, Christel Dubuisson, et al. “RIPK1 Promotes Death Receptor-independent Caspase-8-mediated Apoptosis Under Unresolved ER Stress Conditions.” CELL DEATH & DISEASE 5 (2014): n. pag. Print.
@article{5818685,
  abstract     = {Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress and results in the activation of the unfolded protein response (UPR), which aims at restoring ER homeostasis. However, when the stress is too severe the UPR switches from being a pro-survival response to a pro-death one, and the molecular mechanisms underlying ER stress-mediated death have remained incompletely understood. In this study, we identified receptor interacting protein kinase 1 (RIPK1)-a kinase at the crossroad between life and death downstream of various receptors-as a new regulator of ER stress-induced death. We found that Ripk1-deficient MEFs are protected from apoptosis induced by ER stressors, which is reflected by reduced caspase activation and PARP processing. Interestingly, the pro-apoptotic role of Ripk1 is independent of its kinase activity, is not regulated by its cIAP1/2-mediated ubiquitylation, and does not rely on the direct regulation of JNK or CHOP, two reportedly main players in ER stress-induced death. Instead, we found that ER stress-induced apoptosis in these cells relies on death receptor-independent activation of caspase-8, and identified Ripk1 upstream of caspase-8. However, in contrast to RIPK1-dependent apoptosis downstream of TNFR1, we did not find Ripk1 associated with caspase-8 in a death-inducing complex upon unresolved ER stress. Our data rather suggest that RIPK1 indirectly regulates caspase-8 activation, in part via interaction with the ER stress sensor inositol-requiring protein 1 (IRE1).},
  articleno    = {e1555},
  author       = {Estornes, Yann and Aguileta, Miguel and Dubuisson, Christel and De Keyser, Julie and Goossens, Vera and Kersse, Kristof and Samali, A and Vandenabeele, Peter and Bertrand, Mathieu},
  issn         = {2041-4889},
  journal      = {CELL DEATH \& DISEASE},
  language     = {eng},
  pages        = {11},
  title        = {RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions},
  url          = {http://dx.doi.org/10.1038/cddis.2014.523},
  volume       = {5},
  year         = {2014},
}

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