Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata
- Author
- Tessa Moses (UGent) , Jacob Pollier (UGent) , Ahmad Faizal (UGent) , Sandra Apers, Luc Pieters (UGent) , Johan M Thevelein, Danny Geelen (UGent) and Alain Goossens (UGent)
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- Project
- Abstract
- Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.
- Keywords
- BIOLOGICAL-ACTIVITIES, FUNCTIONAL GENOMICS, LIQUID-CHROMATOGRAPHY, MEDICAGO-TRUNCATULA, SACCHAROMYCES-CEREVISIAE, COMBINATORIAL BIOSYNTHESIS, BETA-AMYRIN SYNTHASE, oxidosqualene cyclase, cytochrome P450, C-28 oxidase, C-16α oxidase, triterpenoid saponin, Maesa lanceolata, MASS-SPECTROMETRY, EVOLUTION, PLANTS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-5810843
- MLA
- Moses, Tessa, et al. “Unravelling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa Lanceolata.” MOLECULAR PLANT, vol. 8, no. 1, 2015, pp. 122–35, doi:10.1016/j.molp.2014.11.004.
- APA
- Moses, T., Pollier, J., Faizal, A., Apers, S., Pieters, L., Thevelein, J. M., … Goossens, A. (2015). Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata. MOLECULAR PLANT, 8(1), 122–135. https://doi.org/10.1016/j.molp.2014.11.004
- Chicago author-date
- Moses, Tessa, Jacob Pollier, Ahmad Faizal, Sandra Apers, Luc Pieters, Johan M Thevelein, Danny Geelen, and Alain Goossens. 2015. “Unravelling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa Lanceolata.” MOLECULAR PLANT 8 (1): 122–35. https://doi.org/10.1016/j.molp.2014.11.004.
- Chicago author-date (all authors)
- Moses, Tessa, Jacob Pollier, Ahmad Faizal, Sandra Apers, Luc Pieters, Johan M Thevelein, Danny Geelen, and Alain Goossens. 2015. “Unravelling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa Lanceolata.” MOLECULAR PLANT 8 (1): 122–135. doi:10.1016/j.molp.2014.11.004.
- Vancouver
- 1.Moses T, Pollier J, Faizal A, Apers S, Pieters L, Thevelein JM, et al. Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata. MOLECULAR PLANT. 2015;8(1):122–35.
- IEEE
- [1]T. Moses et al., “Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata,” MOLECULAR PLANT, vol. 8, no. 1, pp. 122–135, 2015.
@article{5810843, abstract = {{Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.}}, author = {{Moses, Tessa and Pollier, Jacob and Faizal, Ahmad and Apers, Sandra and Pieters, Luc and Thevelein, Johan M and Geelen, Danny and Goossens, Alain}}, issn = {{1674-2052}}, journal = {{MOLECULAR PLANT}}, keywords = {{BIOLOGICAL-ACTIVITIES,FUNCTIONAL GENOMICS,LIQUID-CHROMATOGRAPHY,MEDICAGO-TRUNCATULA,SACCHAROMYCES-CEREVISIAE,COMBINATORIAL BIOSYNTHESIS,BETA-AMYRIN SYNTHASE,oxidosqualene cyclase,cytochrome P450,C-28 oxidase,C-16α oxidase,triterpenoid saponin,Maesa lanceolata,MASS-SPECTROMETRY,EVOLUTION,PLANTS}}, language = {{eng}}, number = {{1}}, pages = {{122--135}}, title = {{Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata}}, url = {{http://doi.org/10.1016/j.molp.2014.11.004}}, volume = {{8}}, year = {{2015}}, }
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