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Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata

Tessa Moses (UGent) , Jacob Pollier (UGent) , Ahmad Faizal (UGent) , Sandra Apers, Luc Pieters (UGent) , Johan M Thevelein, Danny Geelen (UGent) and Alain Goossens (UGent)
(2015) MOLECULAR PLANT. 8(1). p.122-135
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.
Keywords
BIOLOGICAL-ACTIVITIES, FUNCTIONAL GENOMICS, LIQUID-CHROMATOGRAPHY, MEDICAGO-TRUNCATULA, SACCHAROMYCES-CEREVISIAE, COMBINATORIAL BIOSYNTHESIS, BETA-AMYRIN SYNTHASE, oxidosqualene cyclase, cytochrome P450, C-28 oxidase, C-16α oxidase, triterpenoid saponin, Maesa lanceolata, MASS-SPECTROMETRY, EVOLUTION, PLANTS

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Chicago
Moses, Tessa, Jacob Pollier, Ahmad Faizal, Sandra Apers, Luc Pieters, Johan M Thevelein, Danny Geelen, and Alain Goossens. 2015. “Unravelling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa Lanceolata.” Molecular Plant 8 (1): 122–135.
APA
Moses, T., Pollier, J., Faizal, A., Apers, S., Pieters, L., Thevelein, J. M., Geelen, D., et al. (2015). Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata. MOLECULAR PLANT, 8(1), 122–135.
Vancouver
1.
Moses T, Pollier J, Faizal A, Apers S, Pieters L, Thevelein JM, et al. Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata. MOLECULAR PLANT. 2015;8(1):122–35.
MLA
Moses, Tessa, Jacob Pollier, Ahmad Faizal, et al. “Unravelling the Triterpenoid Saponin Biosynthesis of the African Shrub Maesa Lanceolata.” MOLECULAR PLANT 8.1 (2015): 122–135. Print.
@article{5810843,
  abstract     = {Maesasaponins produced by the African shrub Maesa lanceolata are oleanane-type saponins with diverse biological activities. Through a combination of transcript profiling of methyl jasmonate-elicited M. lanceolata shoot cultures, functional analysis in transgenic M. lanceolata plants and the heterologous hosts Medicago truncatula and Saccharomyces cerevisiae, we identified three maesaponin biosynthesis genes. These include a β-amyrin synthase and two cytochrome P450s, CYP716A75 and CYP87D16, which catalyze the C-28 and C-16α oxidations of β-amyrin, respectively.},
  author       = {Moses, Tessa and Pollier, Jacob and Faizal, Ahmad and Apers, Sandra and Pieters, Luc and Thevelein, Johan M and Geelen, Danny and Goossens, Alain},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keywords     = {BIOLOGICAL-ACTIVITIES,FUNCTIONAL GENOMICS,LIQUID-CHROMATOGRAPHY,MEDICAGO-TRUNCATULA,SACCHAROMYCES-CEREVISIAE,COMBINATORIAL BIOSYNTHESIS,BETA-AMYRIN SYNTHASE,oxidosqualene cyclase,cytochrome P450,C-28 oxidase,C-16α oxidase,triterpenoid saponin,Maesa lanceolata,MASS-SPECTROMETRY,EVOLUTION,PLANTS},
  language     = {eng},
  number       = {1},
  pages        = {122--135},
  title        = {Unravelling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata},
  url          = {http://dx.doi.org/10.1016/j.molp.2014.11.004},
  volume       = {8},
  year         = {2015},
}

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