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Refined candidate region for F4ab/ac enterotoxigenic Escherichia coli susceptibility situated proximal to MUC13 in pigs

Tiphanie Goetstouwers, Mario Van Poucke UGent, Wouter Coppieters, Ut Nguyen Van UGent, Vesna Melkebeek, Annelies Coddens UGent, Katleen Van Steendam, Dieter Deforce UGent, Eric Cox UGent and Luc Peelman UGent (2014) PLOS ONE. 9(8).
abstract
F4 enterotoxigenic Escherichia coli (F4 ETEC) are an important cause of diarrhea in neonatal and newly-weaned pigs. Based on the predicted differential O-glycosylation patterns of the 2 MUC13 variants (MUC13A and MUC13B) in F4ac ETEC susceptible and F4ac ETEC resistant pigs, the MUC13 gene was recently proposed as the causal gene for F4ac ETEC susceptibility. Because the absence of MUC13 on Western blot from brush border membrane vesicles of F4ab/acR(+) pigs and the absence of F4ac attachment to immunoprecipitated MUC13 could not support this hypothesis, a new GWAS study was performed using 52 non-adhesive and 68 strong adhesive pigs for F4ab/ac ETEC originating from 5 Belgian farms. A refined candidate region (chr13: 144,810,100-144,993,222) for F4ab/ac ETEC susceptibility was identified with MUC13 adjacent to the distal part of the region. This candidate region lacks annotated genes and contains a sequence gap based on the sequence of the porcine GenomeBuild 10.2. We hypothesize that a porcine orphan gene or trans-acting element present in the identified candidate region has an effect on the glycosylation of F4 binding proteins and therefore determines the F4ab/ac ETEC susceptibility in pigs.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ASSOCIATION, ADHESION, PIGLETS, LINKAGE, VILLI, DIARRHEA, IDENTIFICATION, PURIFIED F4, INTESTINAL RECEPTOR, FIMBRIAE
journal title
PLOS ONE
PLoS One
volume
9
issue
8
article number
e105013
pages
8 pages
Web of Science type
Article
Web of Science id
000340742100059
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
3.234 (2014)
JCR rank
9/57 (2014)
JCR quartile
1 (2014)
ISSN
1932-6203
DOI
10.1371/journal.pone.0105013
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5810291
handle
http://hdl.handle.net/1854/LU-5810291
date created
2015-01-20 09:20:04
date last changed
2016-12-21 15:41:36
@article{5810291,
  abstract     = {F4 enterotoxigenic Escherichia coli (F4 ETEC) are an important cause of diarrhea in neonatal and newly-weaned pigs. Based on the predicted differential O-glycosylation patterns of the 2 MUC13 variants (MUC13A and MUC13B) in F4ac ETEC susceptible and F4ac ETEC resistant pigs, the MUC13 gene was recently proposed as the causal gene for F4ac ETEC susceptibility. Because the absence of MUC13 on Western blot from brush border membrane vesicles of F4ab/acR(+) pigs and the absence of F4ac attachment to immunoprecipitated MUC13 could not support this hypothesis, a new GWAS study was performed using 52 non-adhesive and 68 strong adhesive pigs for F4ab/ac ETEC originating from 5 Belgian farms. A refined candidate region (chr13: 144,810,100-144,993,222) for F4ab/ac ETEC susceptibility was identified with MUC13 adjacent to the distal part of the region. This candidate region lacks annotated genes and contains a sequence gap based on the sequence of the porcine GenomeBuild 10.2. We hypothesize that a porcine orphan gene or trans-acting element present in the identified candidate region has an effect on the glycosylation of F4 binding proteins and therefore determines the F4ab/ac ETEC susceptibility in pigs.},
  articleno    = {e105013},
  author       = {Goetstouwers, Tiphanie and Van Poucke, Mario and Coppieters, Wouter and Nguyen Van, Ut and Melkebeek, Vesna and Coddens, Annelies and Van Steendam, Katleen and Deforce, Dieter and Cox, Eric and Peelman, Luc},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {ASSOCIATION,ADHESION,PIGLETS,LINKAGE,VILLI,DIARRHEA,IDENTIFICATION,PURIFIED F4,INTESTINAL RECEPTOR,FIMBRIAE},
  language     = {eng},
  number       = {8},
  pages        = {8},
  title        = {Refined candidate region for F4ab/ac enterotoxigenic Escherichia coli susceptibility situated proximal to MUC13 in pigs},
  url          = {http://dx.doi.org/10.1371/journal.pone.0105013},
  volume       = {9},
  year         = {2014},
}

Chicago
Goetstouwers, Tiphanie, Mario Van Poucke, Wouter Coppieters, Ut Nguyen Van, Vesna Melkebeek, Annelies Coddens, Katleen Van Steendam, Dieter Deforce, Eric Cox, and Luc Peelman. 2014. “Refined Candidate Region for F4ab/ac Enterotoxigenic Escherichia Coli Susceptibility Situated Proximal to MUC13 in Pigs.” Plos One 9 (8).
APA
Goetstouwers, T., Van Poucke, M., Coppieters, W., Nguyen Van, U., Melkebeek, V., Coddens, A., Van Steendam, K., et al. (2014). Refined candidate region for F4ab/ac enterotoxigenic Escherichia coli susceptibility situated proximal to MUC13 in pigs. PLOS ONE, 9(8).
Vancouver
1.
Goetstouwers T, Van Poucke M, Coppieters W, Nguyen Van U, Melkebeek V, Coddens A, et al. Refined candidate region for F4ab/ac enterotoxigenic Escherichia coli susceptibility situated proximal to MUC13 in pigs. PLOS ONE. 2014;9(8).
MLA
Goetstouwers, Tiphanie, Mario Van Poucke, Wouter Coppieters, et al. “Refined Candidate Region for F4ab/ac Enterotoxigenic Escherichia Coli Susceptibility Situated Proximal to MUC13 in Pigs.” PLOS ONE 9.8 (2014): n. pag. Print.