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Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coli and their fimbriae

Tiphanie Goetstouwers, Mario Van Poucke UGent, Annelies Coddens UGent, Ut Nguyen Van UGent, Vesna Melkebeek, Dieter Deforce UGent, Eric Cox UGent and Luc Peelman UGent (2014) BMC GENETICS. 15.
abstract
Background: Glycosphingolipids (GSLs) are important membrane components composed of a carbohydrate structure attached to a hydrophobic ceramide. They can serve as specific membrane receptors for microbes and microbial products, such as F4 Escherichia coli (F4 ETEC) and isolated F4 fimbriae. The aim of this study was to investigate the hypothesis that variation in genes involved in the assembly of the F4 binding carbohydrate moiety of GSLs (i.e. ARSA, B4GALT6, GAL3ST1, GALC, GBA, GLA, GLB1, GLB1L, NEU1, NEU2, UGCG, UGT8) could account for differential binding of F4 ETEC and their fimbriae. Results: RT-PCR could not reveal any differential expression of the 12 genes in the jejunum of F4 receptor-positive (F4R(+)) and F4 receptor-negative (F4R(-)) pigs. Sequencing the complete open reading frame of the 11 expressed genes (NEU2 was not expressed) identified 72 mutations. Although some of them might have a structural effect, none of them could be associated with a F4R phenotype. Conclusion: We conclude that no regulatory or structural variation in any of the investigated genes is responsible for the genetic susceptibility of pigs towards F4 ETEC.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Glycosphingolipids, Variation, Pig, Binding, F4 Escherichia coli, PIGS, PHENOTYPES, PIGLETS, BREEDS, K88, SUSCEPTIBILITY, EXPRESSION, ADHESIVE, DIARRHEA, F4AB/AC
journal title
BMC GENETICS
BMC Genet.
volume
15
article number
103
pages
5 pages
Web of Science type
Article
Web of Science id
000342596900001
JCR category
GENETICS & HEREDITY
JCR impact factor
2.397 (2014)
JCR rank
91/167 (2014)
JCR quartile
3 (2014)
ISSN
1471-2156
DOI
10.1186/s12863-014-0103-x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
5810275
handle
http://hdl.handle.net/1854/LU-5810275
date created
2015-01-20 09:20:04
date last changed
2016-12-21 15:42:49
@article{5810275,
  abstract     = {Background: Glycosphingolipids (GSLs) are important membrane components composed of a carbohydrate structure attached to a hydrophobic ceramide. They can serve as specific membrane receptors for microbes and microbial products, such as F4 Escherichia coli (F4 ETEC) and isolated F4 fimbriae. The aim of this study was to investigate the hypothesis that variation in genes involved in the assembly of the F4 binding carbohydrate moiety of GSLs (i.e. ARSA, B4GALT6, GAL3ST1, GALC, GBA, GLA, GLB1, GLB1L, NEU1, NEU2, UGCG, UGT8) could account for differential binding of F4 ETEC and their fimbriae. 
Results: RT-PCR could not reveal any differential expression of the 12 genes in the jejunum of F4 receptor-positive (F4R(+)) and F4 receptor-negative (F4R(-)) pigs. Sequencing the complete open reading frame of the 11 expressed genes (NEU2 was not expressed) identified 72 mutations. Although some of them might have a structural effect, none of them could be associated with a F4R phenotype. 
Conclusion: We conclude that no regulatory or structural variation in any of the investigated genes is responsible for the genetic susceptibility of pigs towards F4 ETEC.},
  articleno    = {103},
  author       = {Goetstouwers, Tiphanie and Van Poucke, Mario and Coddens, Annelies and Nguyen Van, Ut and Melkebeek, Vesna and Deforce, Dieter and Cox, Eric and Peelman, Luc},
  issn         = {1471-2156},
  journal      = {BMC GENETICS},
  keyword      = {Glycosphingolipids,Variation,Pig,Binding,F4 Escherichia coli,PIGS,PHENOTYPES,PIGLETS,BREEDS,K88,SUSCEPTIBILITY,EXPRESSION,ADHESIVE,DIARRHEA,F4AB/AC},
  language     = {eng},
  pages        = {5},
  title        = {Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coli and their fimbriae},
  url          = {http://dx.doi.org/10.1186/s12863-014-0103-x},
  volume       = {15},
  year         = {2014},
}

Chicago
Goetstouwers, Tiphanie, Mario Van Poucke, Annelies Coddens, Ut Nguyen Van, Vesna Melkebeek, Dieter Deforce, Eric Cox, and Luc Peelman. 2014. “Variation in 12 Porcine Genes Involved in the Carbohydrate Moiety Assembly of Glycosphingolipids Does Not Account for Differential Binding of F4 Escherichia Coli and Their Fimbriae.” Bmc Genetics 15.
APA
Goetstouwers, T., Van Poucke, M., Coddens, A., Nguyen Van, U., Melkebeek, V., Deforce, D., Cox, E., et al. (2014). Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coli and their fimbriae. BMC GENETICS, 15.
Vancouver
1.
Goetstouwers T, Van Poucke M, Coddens A, Nguyen Van U, Melkebeek V, Deforce D, et al. Variation in 12 porcine genes involved in the carbohydrate moiety assembly of glycosphingolipids does not account for differential binding of F4 Escherichia coli and their fimbriae. BMC GENETICS. 2014;15.
MLA
Goetstouwers, Tiphanie, Mario Van Poucke, Annelies Coddens, et al. “Variation in 12 Porcine Genes Involved in the Carbohydrate Moiety Assembly of Glycosphingolipids Does Not Account for Differential Binding of F4 Escherichia Coli and Their Fimbriae.” BMC GENETICS 15 (2014): n. pag. Print.