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Spatial heterogeneity in degradation characteristics and microbial community composition of pesticide biopurification systems

Pieter Verhagen (UGent) , Chiara Destino (UGent) , Nico Boon (UGent) and Leen De Gelder (UGent)
(2015) JOURNAL OF APPLIED MICROBIOLOGY. 118(2). p.368-378
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Abstract
Aims: To investigate spatial and temporal differences in degradation characteristics and microbial community composition of pesticide biopurification systems. Methods and Results: Pilot-scale biofilters were supplemented with the potato-sprouting suppressant chloropropham. Two biofilters were inoculated with a chloropropham-degrading mixed culture, while the other two were not inoculated. Biodegradation rate, size and composition of the microbial community were monitored during 72days at different biofilter depths. First of all, results showed that inoculation was not necessary to obtain efficient degradation although it shortens the biofilter's start-up period. Secondly, a higher biodegradation rate and chloropropham- and 3-chloroaniline-degrading microbial community size could be seen in the top part of the inoculated as well as the noninoculated biofilters. Finally, analysis of the microbial community composition shows that no clear spatial stratification of the microbial community could be found in any biofilter. However, the microbial diversity increases over time in all biofilters and on all biofilter depths, suggesting that during the time of the experiment, the biofilters develop a broad carrying capacity in which a genetically very diverse range of chloropropham- and 3-chloroaniline-degrading species can thrive. Conclusions: In this study, a vertical gradient of the chloropropham- and 3-chloroaniline-degrading community composition, in terms of density and temporal and spatial diversity, was clearly established and was directly connected to a vertical gradient of chloropropham biodegradation activity. Significance and Impact of the Study: The major part of degradation activity takes place in the top part of the biofilter, suggesting that it could be possible to use shorter biofilter reactors or higher loading rates to treat chloropropham waste streams, making this type of bioremediation technique economically more feasible.
Keywords
biodegradation, 3-chloroaniline, bioremediation, chloropropham, pesticide biopurification system, VOLATILE ORGANIC-COMPOUNDS, PSEUDOMONAS-ALCALIGENES, BACTERIAL CONSORTIUM, REMOVAL EFFICIENCY, SUBSURFACE SOILS, SHOCK LOADS, BIODEGRADATION, 3-CHLOROANILINE, INOCULATION, BIOFILTERS

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Please use this url to cite or link to this publication:

MLA
Verhagen, Pieter, Chiara Destino, Nico Boon, et al. “Spatial Heterogeneity in Degradation Characteristics and Microbial Community Composition of Pesticide Biopurification Systems.” JOURNAL OF APPLIED MICROBIOLOGY 118.2 (2015): 368–378. Print.
APA
Verhagen, Pieter, Destino, C., Boon, N., & De Gelder, L. (2015). Spatial heterogeneity in degradation characteristics and microbial community composition of pesticide biopurification systems. JOURNAL OF APPLIED MICROBIOLOGY, 118(2), 368–378.
Chicago author-date
Verhagen, Pieter, Chiara Destino, Nico Boon, and Leen De Gelder. 2015. “Spatial Heterogeneity in Degradation Characteristics and Microbial Community Composition of Pesticide Biopurification Systems.” Journal of Applied Microbiology 118 (2): 368–378.
Chicago author-date (all authors)
Verhagen, Pieter, Chiara Destino, Nico Boon, and Leen De Gelder. 2015. “Spatial Heterogeneity in Degradation Characteristics and Microbial Community Composition of Pesticide Biopurification Systems.” Journal of Applied Microbiology 118 (2): 368–378.
Vancouver
1.
Verhagen P, Destino C, Boon N, De Gelder L. Spatial heterogeneity in degradation characteristics and microbial community composition of pesticide biopurification systems. JOURNAL OF APPLIED MICROBIOLOGY. 2015;118(2):368–78.
IEEE
[1]
P. Verhagen, C. Destino, N. Boon, and L. De Gelder, “Spatial heterogeneity in degradation characteristics and microbial community composition of pesticide biopurification systems,” JOURNAL OF APPLIED MICROBIOLOGY, vol. 118, no. 2, pp. 368–378, 2015.
@article{5804119,
  abstract     = {Aims: To investigate spatial and temporal differences in degradation characteristics and microbial community composition of pesticide biopurification systems. 
Methods and Results: Pilot-scale biofilters were supplemented with the potato-sprouting suppressant chloropropham. Two biofilters were inoculated with a chloropropham-degrading mixed culture, while the other two were not inoculated. Biodegradation rate, size and composition of the microbial community were monitored during 72days at different biofilter depths. First of all, results showed that inoculation was not necessary to obtain efficient degradation although it shortens the biofilter's start-up period. Secondly, a higher biodegradation rate and chloropropham- and 3-chloroaniline-degrading microbial community size could be seen in the top part of the inoculated as well as the noninoculated biofilters. Finally, analysis of the microbial community composition shows that no clear spatial stratification of the microbial community could be found in any biofilter. However, the microbial diversity increases over time in all biofilters and on all biofilter depths, suggesting that during the time of the experiment, the biofilters develop a broad carrying capacity in which a genetically very diverse range of chloropropham- and 3-chloroaniline-degrading species can thrive. 
Conclusions: In this study, a vertical gradient of the chloropropham- and 3-chloroaniline-degrading community composition, in terms of density and temporal and spatial diversity, was clearly established and was directly connected to a vertical gradient of chloropropham biodegradation activity. 
Significance and Impact of the Study: The major part of degradation activity takes place in the top part of the biofilter, suggesting that it could be possible to use shorter biofilter reactors or higher loading rates to treat chloropropham waste streams, making this type of bioremediation technique economically more feasible.},
  author       = {Verhagen, Pieter and Destino, Chiara and Boon, Nico and De Gelder, Leen},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keywords     = {biodegradation,3-chloroaniline,bioremediation,chloropropham,pesticide biopurification system,VOLATILE ORGANIC-COMPOUNDS,PSEUDOMONAS-ALCALIGENES,BACTERIAL CONSORTIUM,REMOVAL EFFICIENCY,SUBSURFACE SOILS,SHOCK LOADS,BIODEGRADATION,3-CHLOROANILINE,INOCULATION,BIOFILTERS},
  language     = {eng},
  number       = {2},
  pages        = {368--378},
  title        = {Spatial heterogeneity in degradation characteristics and microbial community composition of pesticide biopurification systems},
  url          = {http://dx.doi.org/10.1111/jam.12716},
  volume       = {118},
  year         = {2015},
}

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