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Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout

Annelies Declercq UGent, Koen Chiers UGent, Wim Van Den Broeck UGent, Jeroen Dewulf UGent, Venessa Eeckhaut UGent, Maria Cornelissen UGent, Peter Bossier UGent, Freddy Haesebrouck UGent and Annemie Decostere UGent (2015) VETERINARY RESEARCH. 46.
abstract
The interactions of Flavobacterium columnare isolates of different virulence with the gills of carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum) were investigated. Both fish species were exposed to different high (HV) or low virulence (LV) isolates and sacrificed at seven predetermined times post-challenge. Histopathological and ultrastructural examination of carp and rainbow trout inoculated with the HV-isolate disclosed bacterial invasion and concomitant destruction of the gill tissue, gradually spreading from the filament tips towards the base, with outer membrane vesicles surrounding most bacterial cells. In carp, 5-10% of the fish inoculated with the LV-isolate became moribund and their gill tissue displayed the same features as described for the HV-isolate, albeit to a lesser degree. The bacterial numbers retrieved from the gill tissue were significantly higher for HV- compared to LV-challenged carp and rainbow trout. TUNEL-stained and caspase-3-immunostained gill sections demonstrated significantly higher apoptotic cell counts in carp and rainbow trout challenged with the HV-isolate compared to control animals. Periodic acid-Schiff/alcian blue staining demonstrated a significantly higher total gill goblet cell count for HV- and LV-isolate challenged compared to control carp. Moreover, bacterial clusters were embedded in a neutral matrix while being encased by acid mucins, resembling biofilm formation. Eosinophilic granular cell counts were significantly higher in the HV-isolate compared to LV-isolate inoculated and control carp. The present data indicate a high colonization capacity, and the destructive and apoptotic-promoting features of the HV-isolate, and point towards important dynamic host mucin–F. columnare interactions warranting further research.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BIOFILM FORMATION, TELEOSTEAN FISH, ONCORHYNCHUS-MYKISS, RODLET CELLS, DISEASE, CYPRINUS-CARPIO, FISH PATHOGEN, FLEXIBACTER-COLUMNARIS, CELLS/EOSINOPHILIC GRANULE CELLS, OUTER-MEMBRANE VESICLES
journal title
VETERINARY RESEARCH
Vet. Res.
volume
46
article number
25
pages
16 pages
Web of Science type
Article
Web of Science id
000350376700001
JCR category
VETERINARY SCIENCES
JCR impact factor
2.928 (2015)
JCR rank
3/138 (2015)
JCR quartile
1 (2015)
ISSN
0928-4249
DOI
10.1186/s13567-015-0164-5
project
Study of the early pathogenesis of columnaris disease in freshwaterfish
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
5798866
handle
http://hdl.handle.net/1854/LU-5798866
date created
2015-01-07 17:46:33
date last changed
2017-06-07 14:16:26
@article{5798866,
  abstract     = {The interactions of Flavobacterium columnare isolates of different virulence with the gills of carp (Cyprinus carpio L.) and rainbow trout (Oncorhynchus mykiss Walbaum) were investigated. Both fish species were exposed to different high (HV) or low virulence (LV) isolates and sacrificed at seven predetermined times post-challenge. Histopathological and ultrastructural examination of carp and rainbow trout inoculated with the HV-isolate disclosed bacterial invasion and concomitant destruction of the gill tissue, gradually spreading from the filament tips towards the base, with outer membrane vesicles surrounding most bacterial cells. In carp, 5-10\% of the fish inoculated with the LV-isolate became moribund and their gill tissue displayed the same features as described for the HV-isolate, albeit to a lesser degree. The bacterial numbers retrieved from the gill tissue were significantly higher for HV- compared to LV-challenged carp and rainbow trout. TUNEL-stained and caspase-3-immunostained gill sections demonstrated significantly higher apoptotic cell counts in carp  and rainbow trout challenged with the HV-isolate compared to control animals. Periodic acid-Schiff/alcian blue staining demonstrated a significantly higher total gill goblet cell count for HV- and LV-isolate challenged compared to control carp. Moreover, bacterial clusters were embedded in a neutral matrix while being encased by acid mucins, resembling biofilm formation. Eosinophilic granular cell counts were significantly higher in the HV-isolate compared to LV-isolate inoculated and control carp. The present data indicate a high colonization capacity, and the destructive and apoptotic-promoting features of the HV-isolate, and point towards important dynamic host mucin--F. columnare interactions warranting further research.},
  articleno    = {25},
  author       = {Declercq, Annelies and Chiers, Koen and Van Den Broeck, Wim and Dewulf, Jeroen and Eeckhaut, Venessa and Cornelissen, Maria and Bossier, Peter and Haesebrouck, Freddy and Decostere, Annemie},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {BIOFILM FORMATION,TELEOSTEAN FISH,ONCORHYNCHUS-MYKISS,RODLET CELLS,DISEASE,CYPRINUS-CARPIO,FISH PATHOGEN,FLEXIBACTER-COLUMNARIS,CELLS/EOSINOPHILIC GRANULE CELLS,OUTER-MEMBRANE VESICLES},
  language     = {eng},
  pages        = {16},
  title        = {Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout},
  url          = {http://dx.doi.org/10.1186/s13567-015-0164-5},
  volume       = {46},
  year         = {2015},
}

Chicago
Declercq, Annelies, Koen Chiers, Wim Van Den Broeck, Jeroen Dewulf, Venessa Eeckhaut, Maria Cornelissen, Peter Bossier, Freddy Haesebrouck, and Annemie Decostere. 2015. “Interactions of Highly and Low Virulent Flavobacterium Columnare Isolates with Gill Tissue in Carp and Rainbow Trout.” Veterinary Research 46.
APA
Declercq, Annelies, Chiers, K., Van Den Broeck, W., Dewulf, J., Eeckhaut, V., Cornelissen, M., Bossier, P., et al. (2015). Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout. VETERINARY RESEARCH, 46.
Vancouver
1.
Declercq A, Chiers K, Van Den Broeck W, Dewulf J, Eeckhaut V, Cornelissen M, et al. Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout. VETERINARY RESEARCH. 2015;46.
MLA
Declercq, Annelies, Koen Chiers, Wim Van Den Broeck, et al. “Interactions of Highly and Low Virulent Flavobacterium Columnare Isolates with Gill Tissue in Carp and Rainbow Trout.” VETERINARY RESEARCH 46 (2015): n. pag. Print.