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A multilevel modeling framework to study hepatic perfusion characteristics in case of liver cirrhosis

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Abstract
Liver cirrhosis represents the end-stage of different liver disorders, progressively affecting hepatic architecture, hemodynamics, and function. Morphologically, cirrhosis is characterized by diffuse fibrosis, the conversion of normal liver architecture into structurally abnormal regenerative nodules and the formation of an abundant vascular network. To date, the vascular remodeling and altered hemodynamics due to cirrhosis are still poorly understood, even though they seem to play a pivotal role in cirrhogenesis. This study aims to determine the perfusion characteristics of the cirrhotic circulation using a multilevel modeling approach including computational fluid dynamics (CFD) simulations. Vascular corrosion casting and multilevel micro-CT imaging of a single human cirrhotic liver generated detailed datasets of the hepatic circulation, including typical pathological characteristics of cirrhosis such as shunt vessels and dilated sinusoids. Image processing resulted in anatomically correct 3D reconstructions of the microvasculature up to a diameter of about 500 mu m. Subsequently, two cubic samples (150 x 150 x 150 mu m(3)) were virtually dissected from vascularized zones in between regenerative nodules and applied for CFD simulations to study the altered cirrhotic microperfusion and permeability. Additionally, a conceptual 3D model of the cirrhotic macrocirculation was developed to reveal the hemodynamic impact of regenerative nodules. Our results illustrate that the cirrhotic microcirculation is characterized by an anisotropic permeability showing the highest value in the direction parallel to the central vein (k(d,zz) = 1.68 x 10(-13) m(2) and kd, zz = 7.79 x 10(-13) m(2) for sample 1 and 2, respectively) and lower values in the circumferential (k(d,vv) = 5.78 x 10(-14) m(2) and k(d,vv) = 5.65 x 10(-13) m(2) for sample 1 and 2, respectively) and radial (k(d,rr) = 9.87 x 10(-14) m(2) and k(d,rr) = 5.13 x 10(-13) m(2) for sample 1 and 2, respectively) direction. Overall, the observed permeabilities are markedly higher compared to a normal liver, implying a locally decreased intrahepatic vascular resistance (IVR) probably due to local compensation mechanisms (dilated sinusoids and shunt vessels). These counteract the IVR increase caused by the presence of regenerative nodules and dynamic contraction mechanisms (e.g., stellate cells, NO-concentration, etc.). Our conceptual 3D model of the cirrhotic macrocirculation indicates that regenerative nodules severely increase the IVR beyond about 65 vol. % of regenerative nodules. Numerical modeling allows quantifying perfusion characteristics of the cirrhotic macro-and microcirculation, i.e., the effect of regenerative nodules and compensation mechanisms such as dilated sinusoids and shunt vessels. Future research will focus on the development of models to study time-dependent degenerative adaptation of the cirrhotic macro-and microcirculation.
Keywords
human liver cirrhosis, hepatic perfusion, permeability tensor, computational fluid dynamics, CONTRAST-ENHANCED ULTRASOUND, PORTAL-HYPERTENSION, MICROVASCULAR CHANGES, MICROCIRCULATION, RAT, HEMODYNAMICS, ANGIOGENESIS, FEASIBILITY, CIRCULATION, TOMOGRAPHY

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Chicago
Peeters, Geert, Charlotte Debbaut, Pieter Cornillie, Thomas De Schryver, Diethard Monbaliu, Wim Laleman, and Patrick Segers. 2015. “A Multilevel Modeling Framework to Study Hepatic Perfusion Characteristics in Case of Liver Cirrhosis.” Journal of Biomechanical Engineering-transactions of the Asme 137 (5).
APA
Peeters, Geert, Debbaut, C., Cornillie, P., De Schryver, T., Monbaliu, D., Laleman, W., & Segers, P. (2015). A multilevel modeling framework to study hepatic perfusion characteristics in case of liver cirrhosis. JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 137(5).
Vancouver
1.
Peeters G, Debbaut C, Cornillie P, De Schryver T, Monbaliu D, Laleman W, et al. A multilevel modeling framework to study hepatic perfusion characteristics in case of liver cirrhosis. JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME. 2015;137(5).
MLA
Peeters, Geert, Charlotte Debbaut, Pieter Cornillie, et al. “A Multilevel Modeling Framework to Study Hepatic Perfusion Characteristics in Case of Liver Cirrhosis.” JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME 137.5 (2015): n. pag. Print.
@article{5798069,
  abstract     = {Liver cirrhosis represents the end-stage of different liver disorders, progressively affecting hepatic architecture, hemodynamics, and function. Morphologically, cirrhosis is characterized by diffuse fibrosis, the conversion of normal liver architecture into structurally abnormal regenerative nodules and the formation of an abundant vascular network. To date, the vascular remodeling and altered hemodynamics due to cirrhosis are still poorly understood, even though they seem to play a pivotal role in cirrhogenesis. This study aims to determine the perfusion characteristics of the cirrhotic circulation using a multilevel modeling approach including computational fluid dynamics (CFD) simulations. Vascular corrosion casting and multilevel micro-CT imaging of a single human cirrhotic liver generated detailed datasets of the hepatic circulation, including typical pathological characteristics of cirrhosis such as shunt vessels and dilated sinusoids. Image processing resulted in anatomically correct 3D reconstructions of the microvasculature up to a diameter of about 500 mu m. Subsequently, two cubic samples (150 x 150 x 150 mu m(3)) were virtually dissected from vascularized zones in between regenerative nodules and applied for CFD simulations to study the altered cirrhotic microperfusion and permeability. Additionally, a conceptual 3D model of the cirrhotic macrocirculation was developed to reveal the hemodynamic impact of regenerative nodules. Our results illustrate that the cirrhotic microcirculation is characterized by an anisotropic permeability showing the highest value in the direction parallel to the central vein (k(d,zz) = 1.68 x 10(-13) m(2) and kd, zz = 7.79 x 10(-13) m(2) for sample 1 and 2, respectively) and lower values in the circumferential (k(d,vv) = 5.78 x 10(-14) m(2) and k(d,vv) = 5.65 x 10(-13) m(2) for sample 1 and 2, respectively) and radial (k(d,rr) = 9.87 x 10(-14) m(2) and k(d,rr) = 5.13 x 10(-13) m(2) for sample 1 and 2, respectively) direction. Overall, the observed permeabilities are markedly higher compared to a normal liver, implying a locally decreased intrahepatic vascular resistance (IVR) probably due to local compensation mechanisms (dilated sinusoids and shunt vessels). These counteract the IVR increase caused by the presence of regenerative nodules and dynamic contraction mechanisms (e.g., stellate cells, NO-concentration, etc.). Our conceptual 3D model of the cirrhotic macrocirculation indicates that regenerative nodules severely increase the IVR beyond about 65 vol. \% of regenerative nodules. Numerical modeling allows quantifying perfusion characteristics of the cirrhotic macro-and microcirculation, i.e., the effect of regenerative nodules and compensation mechanisms such as dilated sinusoids and shunt vessels. Future research will focus on the development of models to study time-dependent degenerative adaptation of the cirrhotic macro-and microcirculation.},
  articleno    = {051007},
  author       = {Peeters, Geert and Debbaut, Charlotte and Cornillie, Pieter and De Schryver, Thomas and Monbaliu, Diethard and Laleman, Wim and Segers, Patrick},
  issn         = {0148-0731},
  journal      = {JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME},
  language     = {eng},
  number       = {5},
  pages        = {9},
  title        = {A multilevel modeling framework to study hepatic perfusion characteristics in case of liver cirrhosis},
  url          = {http://dx.doi.org/10.1115/1.4029280},
  volume       = {137},
  year         = {2015},
}

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