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An immunogen synthesis strategy for the development of specific anti-deoxynivalenol monoclonal antibodies

Melanie Sanders (UGent) , Yirong Guo (UGent) , Abhishek Iyer (UGent) , Yara Ruiz Garcia (UGent) , Anastasia Galvita (UGent) , Arne Heyerick (UGent) , Dieter Deforce (UGent) , Martijn Risseeuw (UGent) , Serge Van Calenbergh (UGent) , Marc Bracke (UGent) , et al.
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Abstract
An immunogen synthesis strategy was designed to develop anti-deoxynivalenol (DON) monoclonal antibodies with low cross-reactivity against structurally similar trichothecenes. A total of eight different DON immunogens were synthesised, differing in the type and position of the linker on the DON molecule. After immunisation, antisera from mice immunised with different DON immunogens were checked for the presence of relevant antibodies. Then, both homologous and heterologous enzyme-linked immunosorbent assays (ELISAs) were performed for hybridoma screening. Finally, three monoclonal antibodies against DON and its analogues were generated. In addition, monoclonal antibody 13H1 could recognise DON and its analogues in the order of HT-2 toxin>15-acetyldeoxynivalenol (15-ADON)>DON, with IC50 ranging from 1.14 to 2.13 mu gml(-1). Another monoclonal antibody 10H10 manifested relatively close sensitivities to DON, 3-acetyldeoxynivalenol (3-ADON) and 15-ADON, with IC50 values of 22, 15 and 34ngml(-1), respectively. Using an indirect ELISA format decreases the 10H10 sensitivity to 15-ADON with 92%. A third monoclonal antibody 2A9 showed to be very specific and sensitive to 3-ADON, with IC50 of 0.38ngml(-1). Using both 2A9 and 10H10 monoclonal antibodies allows determining sole DON contamination.
Keywords
monoclonal antibodies, cross-reactivity, immunogens, deoxynivalenol, ELISA, MYCOTOXINS DEOXYNIVALENOL, ENZYME-IMMUNOASSAY, PROTEINS, 15-ACETYLDEOXYNIVALENOL, DERIVATIVES, TOXICITY, ANALOGS, TOXIN

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Chicago
Sanders, Melanie, Yirong Guo, Abhishek Iyer, Yara Ruiz Garcia, Anastasia Galvita, Arne Heyerick, Dieter Deforce, et al. 2014. “An Immunogen Synthesis Strategy for the Development of Specific Anti-deoxynivalenol Monoclonal Antibodies.” Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment 31 (10): 1751–1759.
APA
Sanders, Melanie, Guo, Y., Iyer, A., Ruiz Garcia, Y., Galvita, A., Heyerick, A., Deforce, D., et al. (2014). An immunogen synthesis strategy for the development of specific anti-deoxynivalenol monoclonal antibodies. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 31(10), 1751–1759.
Vancouver
1.
Sanders M, Guo Y, Iyer A, Ruiz Garcia Y, Galvita A, Heyerick A, et al. An immunogen synthesis strategy for the development of specific anti-deoxynivalenol monoclonal antibodies. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT. 2014;31(10):1751–9.
MLA
Sanders, Melanie, Yirong Guo, Abhishek Iyer, et al. “An Immunogen Synthesis Strategy for the Development of Specific Anti-deoxynivalenol Monoclonal Antibodies.” FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT 31.10 (2014): 1751–1759. Print.
@article{5794592,
  abstract     = {An immunogen synthesis strategy was designed to develop anti-deoxynivalenol (DON) monoclonal antibodies with low cross-reactivity against structurally similar trichothecenes. A total of eight different DON immunogens were synthesised, differing in the type and position of the linker on the DON molecule. After immunisation, antisera from mice immunised with different DON immunogens were checked for the presence of relevant antibodies. Then, both homologous and heterologous enzyme-linked immunosorbent assays (ELISAs) were performed for hybridoma screening. Finally, three monoclonal antibodies against DON and its analogues were generated. In addition, monoclonal antibody 13H1 could recognise DON and its analogues in the order of HT-2 toxin{\textrangle}15-acetyldeoxynivalenol (15-ADON){\textrangle}DON, with IC50 ranging from 1.14 to 2.13 mu gml(-1). Another monoclonal antibody 10H10 manifested relatively close sensitivities to DON, 3-acetyldeoxynivalenol (3-ADON) and 15-ADON, with IC50 values of 22, 15 and 34ngml(-1), respectively. Using an indirect ELISA format decreases the 10H10 sensitivity to 15-ADON with 92\%. A third monoclonal antibody 2A9 showed to be very specific and sensitive to 3-ADON, with IC50 of 0.38ngml(-1). Using both 2A9 and 10H10 monoclonal antibodies allows determining sole DON contamination.},
  author       = {Sanders, Melanie and Guo, Yirong and Iyer, Abhishek and Ruiz Garcia, Yara and Galvita, Anastasia and Heyerick, Arne and Deforce, Dieter and Risseeuw, Martijn and Van Calenbergh, Serge and Bracke, Marc and Eremin, Sergei and Madder, Annemieke and De Saeger, Sarah},
  issn         = {1944-0049},
  journal      = {FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE \& RISK ASSESSMENT},
  language     = {eng},
  number       = {10},
  pages        = {1751--1759},
  title        = {An immunogen synthesis strategy for the development of specific anti-deoxynivalenol monoclonal antibodies},
  url          = {http://dx.doi.org/10.1080/19440049.2014.955887},
  volume       = {31},
  year         = {2014},
}

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