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The BCESM genomic region contains a regulator involved in quorum sensing and persistence in Burkholderia cenocepacia J2315

Heleen Van Acker (UGent) , Evelien De Canck (UGent) , Filip Van Nieuwerburgh (UGent) , Andrea Sass (UGent) , Dieter Deforce (UGent) , Hans Nelis (UGent) and Tom Coenye (UGent)
(2014) FUTURE MICROBIOLOGY. 9(7). p.845-860
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Abstract
Aim: In this study, we investigated the function of BCAM0257-8-9 located in the BCESM region of the Burkholderia cenocepacia J2315 genome. Materials & methods: Differential RNA sequencing was used to determine transcription start sites. The phenotype of overexpression mutants was studied and a transcriptome analysis of the BCAM0258 overexpression mutant was performed. Results: BCAM0257 and BCAM0258 were identified as belonging to an operon, positively regulated by BCAM0259. We found that this operon is involved in persistence and that BCAM0258 functions as a regulator influencing quorum sensing and activating pathways related to iron acquisition and biofilm formation. Overexpression of BCAM0257 increased virulence. Conclusion: The BCESM genomic region contains an operon that contributes to quorum sensing and is involved in persistence, biofilm formation and virulence. BCAM0257-8-9 is found in all sequenced B. cenocepacia ET12 genomes and these results may help explain why infections with strains of the B. cenocepacia ET12 lineage are difficult to treat.
Keywords
biofilm, BCESM, Burkholderia, persistence, quorum sensing, TA-modules, TOXIN-ANTITOXIN SYSTEMS, ESCHERICHIA-COLI, CEPACIA COMPLEX, MULTIDRUG TOLERANCE, BACTERIAL BIOFILMS, PLASMID, REGULATOR, CELLS, DNA, EXPRESSION

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Chicago
Van Acker, Heleen, Evelien De Canck, Filip Van Nieuwerburgh, Andrea Sass, Dieter Deforce, Hans Nelis, and Tom Coenye. 2014. “The BCESM Genomic Region Contains a Regulator Involved in Quorum Sensing and Persistence in Burkholderia Cenocepacia J2315.” Future Microbiology 9 (7): 845–860.
APA
Van Acker, Heleen, De Canck, E., Van Nieuwerburgh, F., Sass, A., Deforce, D., Nelis, H., & Coenye, T. (2014). The BCESM genomic region contains a regulator involved in quorum sensing and persistence in Burkholderia cenocepacia J2315. FUTURE MICROBIOLOGY, 9(7), 845–860.
Vancouver
1.
Van Acker H, De Canck E, Van Nieuwerburgh F, Sass A, Deforce D, Nelis H, et al. The BCESM genomic region contains a regulator involved in quorum sensing and persistence in Burkholderia cenocepacia J2315. FUTURE MICROBIOLOGY. 2014;9(7):845–60.
MLA
Van Acker, Heleen, Evelien De Canck, Filip Van Nieuwerburgh, et al. “The BCESM Genomic Region Contains a Regulator Involved in Quorum Sensing and Persistence in Burkholderia Cenocepacia J2315.” FUTURE MICROBIOLOGY 9.7 (2014): 845–860. Print.
@article{5793692,
  abstract     = {Aim: In this study, we investigated the function of BCAM0257-8-9 located in the BCESM region of the Burkholderia cenocepacia J2315 genome.
Materials \& methods: Differential RNA sequencing was used to determine transcription start sites. The phenotype of overexpression mutants was studied and a transcriptome analysis of the BCAM0258 overexpression mutant was performed.
Results: BCAM0257 and BCAM0258 were identified as belonging to an operon, positively regulated by BCAM0259. We found that this operon is involved in persistence and that BCAM0258 functions as a regulator influencing quorum sensing and activating pathways related to iron acquisition and biofilm formation. Overexpression of BCAM0257 increased virulence.
Conclusion: The BCESM genomic region contains an operon that contributes to quorum sensing and is involved in persistence, biofilm formation and virulence. BCAM0257-8-9 is found in all sequenced B. cenocepacia ET12 genomes and these results may help explain why infections with strains of the B. cenocepacia ET12 lineage are difficult to treat.},
  author       = {Van Acker, Heleen and De Canck, Evelien and Van Nieuwerburgh, Filip and Sass, Andrea and Deforce, Dieter and Nelis, Hans and Coenye, Tom},
  issn         = {1746-0913},
  journal      = {FUTURE MICROBIOLOGY},
  language     = {eng},
  number       = {7},
  pages        = {845--860},
  title        = {The BCESM genomic region contains a regulator involved in quorum sensing and persistence in Burkholderia cenocepacia J2315},
  url          = {http://dx.doi.org/10.2217/FMB.14.54},
  volume       = {9},
  year         = {2014},
}

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