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Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy

(2014) PLOS ONE. 9(4).
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Abstract
Photosensitizers (PSs) are of crucial importance in the effectiveness of photodynamic therapy (PDT) for cancer. Due to their high reactive oxygen species production and strong absorption in the wavelength range between 650 and 850 nm, where tissue light penetration is rather high, phthalocyanines (Pcs) have been studied as PSs of excellence. In this work, we report the evaluation of a phthalocyanine surrounded by a carbohydrate shell of sixteen galactose units distributed in a dendritic manner (PcGal(16)) as a new and efficient third generation PSs for PDT against two bladder cancer cell lines, HT-1376 and UM-UC-3. Here, we define the role of galacto-dendritic units in promoting the uptake of a Pc through interaction with GLUT1 and galectin-1. The photoactivation of PcGal(16) induces cell death by generating oxidative stress. Although PDT with PcGal(16) induces an increase on the activity of antioxidant enzymes immediately after PDT, bladder cancer cells are unable to recover from the PDT-induced damage effects for at least 72 h after treatment. PcGal(16) co-localization with galectin-1 and GLUT1 and/or generation of oxidative stress after PcGal(16) photoactivation induces changes in the levels of these proteins. Knockdown of galectin-1 and GLUT1, via small interfering RNA (siRNA), in bladder cancer cells decreases intracellular uptake and phototoxicity of PcGal(16). The results reported herein show PcGal(16) as a promising therapeutic agent for the treatment of bladder cancer, which is the fifth most common type of cancer with the highest rate of recurrence of any cancer.
Keywords
GLYCODENDRIMERIC PHENYLPORPHYRINS, PHOTOPHYSICAL PROPERTIES, SUPEROXIDE-DISMUTASE, CONJUGATED CHLORINS, ANTICANCER DRUGS, CELL RECOGNITION, TRANSPORT SYSTEM, CANCER-CELLS, EXPRESSION, PHOTOSENSITIZERS

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Citation

Please use this url to cite or link to this publication:

MLA
Pereira, Patrícia MR, Sandrina Silva, José AS Cavaleiro, et al. “Galactodendritic Phthalocyanine Targets Carbohydrate-binding Proteins Enhancing Photodynamic Therapy.” PLOS ONE 9.4 (2014): n. pag. Print.
APA
Pereira, Patrícia MR, Silva, S., Cavaleiro, J. A., Ribeiro, C. A., Tomé, J., & Fernandes, R. (2014). Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy. PLOS ONE, 9(4).
Chicago author-date
Pereira, Patrícia MR, Sandrina Silva, José AS Cavaleiro, Carlos AF Ribeiro, João Tomé, and Rosa Fernandes. 2014. “Galactodendritic Phthalocyanine Targets Carbohydrate-binding Proteins Enhancing Photodynamic Therapy.” Plos One 9 (4).
Chicago author-date (all authors)
Pereira, Patrícia MR, Sandrina Silva, José AS Cavaleiro, Carlos AF Ribeiro, João Tomé, and Rosa Fernandes. 2014. “Galactodendritic Phthalocyanine Targets Carbohydrate-binding Proteins Enhancing Photodynamic Therapy.” Plos One 9 (4).
Vancouver
1.
Pereira PM, Silva S, Cavaleiro JA, Ribeiro CA, Tomé J, Fernandes R. Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy. PLOS ONE. 2014;9(4).
IEEE
[1]
P. M. Pereira, S. Silva, J. A. Cavaleiro, C. A. Ribeiro, J. Tomé, and R. Fernandes, “Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy,” PLOS ONE, vol. 9, no. 4, 2014.
@article{5786569,
  abstract     = {Photosensitizers (PSs) are of crucial importance in the effectiveness of photodynamic therapy (PDT) for cancer. Due to their high reactive oxygen species production and strong absorption in the wavelength range between 650 and 850 nm, where tissue light penetration is rather high, phthalocyanines (Pcs) have been studied as PSs of excellence. In this work, we report the evaluation of a phthalocyanine surrounded by a carbohydrate shell of sixteen galactose units distributed in a dendritic manner (PcGal(16)) as a new and efficient third generation PSs for PDT against two bladder cancer cell lines, HT-1376 and UM-UC-3. Here, we define the role of galacto-dendritic units in promoting the uptake of a Pc through interaction with GLUT1 and galectin-1. The photoactivation of PcGal(16) induces cell death by generating oxidative stress. Although PDT with PcGal(16) induces an increase on the activity of antioxidant enzymes immediately after PDT, bladder cancer cells are unable to recover from the PDT-induced damage effects for at least 72 h after treatment. PcGal(16) co-localization with galectin-1 and GLUT1 and/or generation of oxidative stress after PcGal(16) photoactivation induces changes in the levels of these proteins. Knockdown of galectin-1 and GLUT1, via small interfering RNA (siRNA), in bladder cancer cells decreases intracellular uptake and phototoxicity of PcGal(16). The results reported herein show PcGal(16) as a promising therapeutic agent for the treatment of bladder cancer, which is the fifth most common type of cancer with the highest rate of recurrence of any cancer.},
  articleno    = {e95529},
  author       = {Pereira, Patrícia MR and Silva, Sandrina and Cavaleiro, José AS and Ribeiro, Carlos AF and Tomé, João and Fernandes, Rosa},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {GLYCODENDRIMERIC PHENYLPORPHYRINS,PHOTOPHYSICAL PROPERTIES,SUPEROXIDE-DISMUTASE,CONJUGATED CHLORINS,ANTICANCER DRUGS,CELL RECOGNITION,TRANSPORT SYSTEM,CANCER-CELLS,EXPRESSION,PHOTOSENSITIZERS},
  language     = {eng},
  number       = {4},
  pages        = {13},
  title        = {Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy},
  url          = {http://dx.doi.org/10.1371/journal.pone.0095529},
  volume       = {9},
  year         = {2014},
}

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