Individual commitment to a group effect: strengths and weaknesses of bovine embryo group culture
- Author
- Eline Wydooghe (UGent) , Leen Vandaele, Sofie Piepers (UGent) , Jeroen Dewulf (UGent) , Etienne Van den Abbeel (UGent) , Petra De Sutter (UGent) and Ann Van Soom (UGent)
- Organization
- Abstract
- Recently, new culture devices such as Corral and Primo Vision dishes have been designed for the culture of human embryos to allow the combination of group culture plus follow-up of individual embryos. Bovine inseminated oocytes were allocated to Primo Vision dishes, Corral dishes, individual culture or classical group culture. Blastocyst development in Primo Vision dishes was similar to classical group culture (34.3 and 39.0% respectively), and better than Corral dishes or individual culture (28.9 and 28.5% respectively). In Primo Vision dishes, a higher number of 'slow' embryos developed to the blastocyst stage compared with their individually cultured counterparts, while no differences were observed for 'fast' embryos. 'Slow' embryos in a 'standard drop' had a higher chance of becoming a blastocyst compared with individual culture (OR: 2.3), whereas blastulation of 'fast' embryos was less efficient in a 'delayed drop' than in individual culture (OR: 0.3). The number of non-cleaved embryos in Primo Vision dishes did not negatively influence blastocyst development. Likewise, removing non-cleaved embryos (NC removed) and regrouping the cleaved embryos afterwards (ReGR) did not affect blastocyst development and quality compared with group culture in Primo Vision dishes (CTRL, 31.6%, NC removed, 29.3% and ReGR, 29.6%). The experiments revealed that group culture of bovine embryos in Primo Vision dishes is superior to individual culture, primarily because of the higher blastocyst rate achieved by slow embryos. Non-cleaved or arrested embryos do not hamper the ability of co-cultured bovine embryos to reach the blastocyst stage in group culture.
- Keywords
- CUMULUS CELL COCULTURE, IN-VITRO DEVELOPMENT, VOLUME, BLASTOCYSTS, OOCYTES, VIVO, PREIMPLANTATION EMBRYOS, MOUSE EMBRYOS, DENSITY, EXPRESSION
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 1.12 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-5785084
- MLA
- Wydooghe, Eline, et al. “Individual Commitment to a Group Effect: Strengths and Weaknesses of Bovine Embryo Group Culture.” REPRODUCTION, vol. 148, no. 5, 2014, pp. 519–29, doi:10.1530/REP-14-0213.
- APA
- Wydooghe, E., Vandaele, L., Piepers, S., Dewulf, J., Van den Abbeel, E., De Sutter, P., & Van Soom, A. (2014). Individual commitment to a group effect: strengths and weaknesses of bovine embryo group culture. REPRODUCTION, 148(5), 519–529. https://doi.org/10.1530/REP-14-0213
- Chicago author-date
- Wydooghe, Eline, Leen Vandaele, Sofie Piepers, Jeroen Dewulf, Etienne Van den Abbeel, Petra De Sutter, and Ann Van Soom. 2014. “Individual Commitment to a Group Effect: Strengths and Weaknesses of Bovine Embryo Group Culture.” REPRODUCTION 148 (5): 519–29. https://doi.org/10.1530/REP-14-0213.
- Chicago author-date (all authors)
- Wydooghe, Eline, Leen Vandaele, Sofie Piepers, Jeroen Dewulf, Etienne Van den Abbeel, Petra De Sutter, and Ann Van Soom. 2014. “Individual Commitment to a Group Effect: Strengths and Weaknesses of Bovine Embryo Group Culture.” REPRODUCTION 148 (5): 519–529. doi:10.1530/REP-14-0213.
- Vancouver
- 1.Wydooghe E, Vandaele L, Piepers S, Dewulf J, Van den Abbeel E, De Sutter P, et al. Individual commitment to a group effect: strengths and weaknesses of bovine embryo group culture. REPRODUCTION. 2014;148(5):519–29.
- IEEE
- [1]E. Wydooghe et al., “Individual commitment to a group effect: strengths and weaknesses of bovine embryo group culture,” REPRODUCTION, vol. 148, no. 5, pp. 519–529, 2014.
@article{5785084, abstract = {{Recently, new culture devices such as Corral and Primo Vision dishes have been designed for the culture of human embryos to allow the combination of group culture plus follow-up of individual embryos. Bovine inseminated oocytes were allocated to Primo Vision dishes, Corral dishes, individual culture or classical group culture. Blastocyst development in Primo Vision dishes was similar to classical group culture (34.3 and 39.0% respectively), and better than Corral dishes or individual culture (28.9 and 28.5% respectively). In Primo Vision dishes, a higher number of 'slow' embryos developed to the blastocyst stage compared with their individually cultured counterparts, while no differences were observed for 'fast' embryos. 'Slow' embryos in a 'standard drop' had a higher chance of becoming a blastocyst compared with individual culture (OR: 2.3), whereas blastulation of 'fast' embryos was less efficient in a 'delayed drop' than in individual culture (OR: 0.3). The number of non-cleaved embryos in Primo Vision dishes did not negatively influence blastocyst development. Likewise, removing non-cleaved embryos (NC removed) and regrouping the cleaved embryos afterwards (ReGR) did not affect blastocyst development and quality compared with group culture in Primo Vision dishes (CTRL, 31.6%, NC removed, 29.3% and ReGR, 29.6%). The experiments revealed that group culture of bovine embryos in Primo Vision dishes is superior to individual culture, primarily because of the higher blastocyst rate achieved by slow embryos. Non-cleaved or arrested embryos do not hamper the ability of co-cultured bovine embryos to reach the blastocyst stage in group culture.}}, author = {{Wydooghe, Eline and Vandaele, Leen and Piepers, Sofie and Dewulf, Jeroen and Van den Abbeel, Etienne and De Sutter, Petra and Van Soom, Ann}}, issn = {{1470-1626}}, journal = {{REPRODUCTION}}, keywords = {{CUMULUS CELL COCULTURE,IN-VITRO DEVELOPMENT,VOLUME,BLASTOCYSTS,OOCYTES,VIVO,PREIMPLANTATION EMBRYOS,MOUSE EMBRYOS,DENSITY,EXPRESSION}}, language = {{eng}}, number = {{5}}, pages = {{519--529}}, title = {{Individual commitment to a group effect: strengths and weaknesses of bovine embryo group culture}}, url = {{http://doi.org/10.1530/REP-14-0213}}, volume = {{148}}, year = {{2014}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: