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Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil

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Abstract
Root-nodule bacteria were isolated from lnga laurina (Sw.) Willd. growing in the Cerrado Amazon region, State of Roraima, Brazil. The 16S rRNA gene sequences of six strains (BR 10250(T), BR 10248, BR 10249, BR 10251, BR 10252 and BR 10253) showed low similarities with currently described species of the genus Bradyrhizobium. Phylogenetic analyses of sequences of five housekeeping genes (dnaK, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EKO5(T) to be the closest type strain (97.4% sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with the major components C-16:0 and summed feature 8 (C-18:1 omega 6c/C-18:1 omega 7c)], the slow growth rate and carbon compound utilization patterns supported the assignment of our strains to the genus Bradyrhizobium. Results from DNA DNA hybridizations and physiological traits differentiated our strains from the closest related species of the genus Bradyrhizobium with validly published names. Sequences of symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped together with those of B. iriomotense EKO5(T) and Bradyrhizobium sp. strains BR 6610 (used as a commercial inoculant for Inga marginata in Brazil) and TUXTLAS-10 (previously observed in Central America). Based on these data, the six strains represent a novel species, for which the name Bradyrhizobium ingae sp. nov. is proposed. The type strain is BR 10250(T) (=HAMBI 3600(T)).
Keywords
DEOXYRIBONUCLEIC-ACID, BACTERIA, BRAZIL, ROOT, DNA, HYBRIDIZATION, STRAINS, GENE, RHIZOBIUM, SINORHIZOBIUM

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Citation

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MLA
da Silva, Krisle et al. “Bradyrhizobium Ingae Sp. Nov., Isolated from Effective Nodules of Inga Laurina Grown in Cerrado Soil.” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 64.10 (2014): 3395–3401. Print.
APA
da Silva, K., De Meyer, S., Rouws, L. F., Farias, E. N., dos Santos, M. A., O’Hara, G., Ardley, J. K., et al. (2014). Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 64(10), 3395–3401.
Chicago author-date
da Silva, Krisle, Sofie De Meyer, Luc FM Rouws, Eliane NC Farias, Marco AO dos Santos, Graham O’Hara, Julie K Ardley, Anne Willems, Rosa Maria Pitard, and Jerri E Zilli. 2014. “Bradyrhizobium Ingae Sp. Nov., Isolated from Effective Nodules of Inga Laurina Grown in Cerrado Soil.” International Journal of Systematic and Evolutionary Microbiology 64 (10): 3395–3401.
Chicago author-date (all authors)
da Silva, Krisle, Sofie De Meyer, Luc FM Rouws, Eliane NC Farias, Marco AO dos Santos, Graham O’Hara, Julie K Ardley, Anne Willems, Rosa Maria Pitard, and Jerri E Zilli. 2014. “Bradyrhizobium Ingae Sp. Nov., Isolated from Effective Nodules of Inga Laurina Grown in Cerrado Soil.” International Journal of Systematic and Evolutionary Microbiology 64 (10): 3395–3401.
Vancouver
1.
da Silva K, De Meyer S, Rouws LF, Farias EN, dos Santos MA, O’Hara G, et al. Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY. 2014;64(10):3395–401.
IEEE
[1]
K. da Silva et al., “Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil,” INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, vol. 64, no. 10, pp. 3395–3401, 2014.
@article{5783723,
  abstract     = {Root-nodule bacteria were isolated from lnga laurina (Sw.) Willd. growing in the Cerrado Amazon region, State of Roraima, Brazil. The 16S rRNA gene sequences of six strains (BR 10250(T), BR 10248, BR 10249, BR 10251, BR 10252 and BR 10253) showed low similarities with currently described species of the genus Bradyrhizobium. Phylogenetic analyses of sequences of five housekeeping genes (dnaK, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EKO5(T) to be the closest type strain (97.4% sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with the major components C-16:0 and summed feature 8 (C-18:1 omega 6c/C-18:1 omega 7c)], the slow growth rate and carbon compound utilization patterns supported the assignment of our strains to the genus Bradyrhizobium. Results from DNA DNA hybridizations and physiological traits differentiated our strains from the closest related species of the genus Bradyrhizobium with validly published names. Sequences of symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped together with those of B. iriomotense EKO5(T) and Bradyrhizobium sp. strains BR 6610 (used as a commercial inoculant for Inga marginata in Brazil) and TUXTLAS-10 (previously observed in Central America). Based on these data, the six strains represent a novel species, for which the name Bradyrhizobium ingae sp. nov. is proposed. The type strain is BR 10250(T) (=HAMBI 3600(T)).},
  author       = {da Silva, Krisle and De Meyer, Sofie and Rouws, Luc FM and Farias, Eliane NC and dos Santos, Marco AO and O’Hara, Graham and Ardley, Julie K and Willems, Anne and Pitard, Rosa Maria and Zilli, Jerri E},
  issn         = {1466-5026},
  journal      = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY},
  keywords     = {DEOXYRIBONUCLEIC-ACID,BACTERIA,BRAZIL,ROOT,DNA,HYBRIDIZATION,STRAINS,GENE,RHIZOBIUM,SINORHIZOBIUM},
  language     = {eng},
  number       = {10},
  pages        = {3395--3401},
  title        = {Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil},
  url          = {http://dx.doi.org/10.1099/ijs.0.063727-0},
  volume       = {64},
  year         = {2014},
}

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