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Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?

(2014) RADIATION PROTECTION DOSIMETRY. 159(1-4). p.87-94
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Abstract
Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution sigma(2)/A mu obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.
Keywords
DOSIMETRY, IRRADIATION, BIODOSIMETRY, IMAGE-ANALYSIS, DICENTRIC CHROMOSOMES, TOOL, LYMPHOCYTES, RADIATION, NETWORK, EXPOSURE

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Citation

Please use this url to cite or link to this publication:

Chicago
Thierens, Hubert, Anne Vral, Charlot Vandevoorde, Veerle Vandersickel, Virginie de Gelder, H Romm, U Oestreicher, et al. 2014. “Is a Semi-automated Approach Indicated in the Application of the Automated Micronucleus Assay for Triage Purposes?” Radiation Protection Dosimetry 159 (1-4): 87–94.
APA
Thierens, H., Vral, A., Vandevoorde, C., Vandersickel, V., de Gelder, V., Romm, H., Oestreicher, U., et al. (2014). Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes? RADIATION PROTECTION DOSIMETRY, 159(1-4), 87–94.
Vancouver
1.
Thierens H, Vral A, Vandevoorde C, Vandersickel V, de Gelder V, Romm H, et al. Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes? RADIATION PROTECTION DOSIMETRY. 2014;159(1-4):87–94.
MLA
Thierens, Hubert, Anne Vral, Charlot Vandevoorde, et al. “Is a Semi-automated Approach Indicated in the Application of the Automated Micronucleus Assay for Triage Purposes?” RADIATION PROTECTION DOSIMETRY 159.1-4 (2014): 87–94. Print.
@article{5779920,
  abstract     = {Within the EU MULTIBIODOSE project, the automated micronucleus (MN) assay was optimised for population triage in large-scale radiological emergencies. For MN scoring, two approaches were applied using the Metafer4 platform (MetaSystems, Germany): fully automated scoring and semi-automated scoring with visual inspection of the gallery of MN-positive objects. Dose-response curves were established for acute and protracted whole-body and partial-body exposures. A database of background MN yields was set up, allowing determination of the dose detection threshold in both scoring modes. An analysis of the overdispersion of the MN frequency distribution sigma(2)/A mu obtained by semi-automated scoring showed that the value of this parameter represents a reliability check of the calculated equivalent total body dose in case the accident overexposure is a partial-body exposure. The elaborated methodology was validated in an accident training exercise. Overall, the semi-automated scoring procedure represents important added value to the automated MN assay.},
  author       = {Thierens, Hubert and Vral, Anne and Vandevoorde, Charlot and Vandersickel, Veerle and de Gelder, Virginie and Romm, H and Oestreicher, U and Rothkamm, K and Barnard, S and Ainsbury, E and Sommer, S and Beinke, C and Wojcik, A},
  issn         = {0144-8420},
  journal      = {RADIATION PROTECTION DOSIMETRY},
  language     = {eng},
  number       = {1-4},
  pages        = {87--94},
  title        = {Is a semi-automated approach indicated in the application of the automated micronucleus assay for triage purposes?},
  url          = {http://dx.doi.org/10.1093/rpd/ncu130},
  volume       = {159},
  year         = {2014},
}

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