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Thiouracil-forming bacteria identified and characterized upon porcine in vitro digestion of Brassicaceae feed

Julie Kiebooms, Jella Wauters UGent, Julie Vanden Bussche, Kurt Houf UGent, Paul De Vos UGent, Stefanie Van Trappen, Ilse Cleenwerck UGent and Lynn Vanhaecke UGent (2014) APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 80(23). p.7433-7442
abstract
In recent years, the frequent detection of the banned thyreostat thiouracil (TU) in livestock urine has been related to endogenous TU formation following digestion of glucosinolate-rich Brassicaceae crops. Recently, it was demonstrated that, upon in vitro digestion of Brassicaceae, fecal bacteria induce TU detection in livestock (porcine livestock > bovines). Therefore, the present study was intended to isolate and identify bacteria involved in this intestinal TU formation upon Brassicaceae digestion and to gain more insight into the underlying mechanism in porcine livestock. Twenty porcine fecal inocula (gilts and multiparous sows) were assessed through static in vitro colonic-digestion simulations with rapeseed. After derivatization and extraction of the fecal suspensions, TU was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS2). On average, lower TU concentrations were observed in fecal colonic simulations in gilts (8.35 ng g-1 rapeseed +- 3.42 [mean +- standard deviation]) than in multiparous sows (52.63 ng g-1 +- 16.17), which correlates with maturation of the gut microbial population with age. Further exploration of the mechanism showed cell-dependent activity of the microbial conversion and sustained TU-forming activity after subjection of the fecal inoculum to moderate heat over a time span of up to 30 min. Finally, nine TU-producing bacterial species were successfully isolated and identified by a combination of biochemical and molecular techniques as Escherichia coli (n = 5), Lactobacillus reuteri (n = 2), Enterococcus faecium (n = 1), and Salmonella enterica subsp. arizonae (n = 1). This report demonstrates that endogenous formation of TU is Brassicaceae induced and occurs under colonic conditions most likely through myrosinase-like enzyme activity expressed by different common intestinal bacterial species.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LC-MS2, rapeseed, endogenous thiouracil, static in vitro colonic digestion simulations, DNA-sequencing, pig, APLASIA-CUTIS, MASS-SPECTROMETRY, GLUCOSINOLATE CONTENT, ESCHERICHIA-COLI, RAPESEED MEAL TOXICITY, SP NOV., LACTOBACILLUS, PIGS, RATS, SINIGRIN
journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Appl. Environ. Microbiol.
volume
80
issue
23
pages
7433 - 7442
Web of Science type
Article
Web of Science id
000344163700034
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.668 (2014)
JCR rank
34/163 (2014)
JCR quartile
1 (2014)
ISSN
0099-2240
DOI
10.1128/AEM.02370-14
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5775832
handle
http://hdl.handle.net/1854/LU-5775832
date created
2014-12-08 14:05:56
date last changed
2016-12-19 15:39:51
@article{5775832,
  abstract     = {In recent years, the frequent detection of the banned thyreostat thiouracil (TU) in livestock urine has been related to endogenous TU formation following digestion of glucosinolate-rich Brassicaceae crops. Recently, it was demonstrated that, upon in vitro digestion of Brassicaceae, fecal bacteria induce TU detection in livestock (porcine livestock {\textrangle} bovines). Therefore, the present study was intended to isolate and identify bacteria involved in this intestinal TU formation upon Brassicaceae digestion and to gain more insight into the underlying mechanism in porcine livestock. Twenty porcine fecal inocula (gilts and multiparous sows) were assessed through static in vitro colonic-digestion simulations with rapeseed. After derivatization and extraction of the fecal suspensions, TU was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS2). On average, lower TU concentrations were observed in fecal colonic simulations in gilts (8.35 ng g-1 rapeseed +- 3.42 [mean +- standard deviation]) than in multiparous sows (52.63 ng g-1 +- 16.17), which correlates with maturation of the gut microbial population with age. Further exploration of the mechanism showed cell-dependent activity of the microbial conversion and sustained TU-forming activity after subjection of the fecal inoculum to moderate heat over a time span of up to 30 min. Finally, nine TU-producing bacterial species were successfully isolated and identified by a combination of biochemical and molecular techniques as Escherichia coli (n = 5), Lactobacillus reuteri (n = 2), Enterococcus faecium (n = 1), and Salmonella enterica subsp. arizonae (n = 1). This report demonstrates that endogenous formation of TU is Brassicaceae induced and occurs under colonic conditions most likely through myrosinase-like enzyme activity expressed by different common intestinal bacterial species.},
  author       = {Kiebooms, Julie and Wauters, Jella and Vanden Bussche, Julie and Houf, Kurt and De Vos, Paul and Van Trappen, Stefanie and Cleenwerck, Ilse and Vanhaecke, Lynn},
  issn         = {0099-2240},
  journal      = {APPLIED AND ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {LC-MS2,rapeseed,endogenous thiouracil,static in vitro colonic digestion simulations,DNA-sequencing,pig,APLASIA-CUTIS,MASS-SPECTROMETRY,GLUCOSINOLATE CONTENT,ESCHERICHIA-COLI,RAPESEED MEAL TOXICITY,SP NOV.,LACTOBACILLUS,PIGS,RATS,SINIGRIN},
  language     = {eng},
  number       = {23},
  pages        = {7433--7442},
  title        = {Thiouracil-forming bacteria identified and characterized upon porcine in vitro digestion of Brassicaceae feed},
  url          = {http://dx.doi.org/10.1128/AEM.02370-14},
  volume       = {80},
  year         = {2014},
}

Chicago
Kiebooms, Julie, Jella Wauters, Julie Vanden Bussche, Kurt Houf, Paul De Vos, Stefanie Van Trappen, Ilse Cleenwerck, and Lynn Vanhaecke. 2014. “Thiouracil-forming Bacteria Identified and Characterized Upon Porcine in Vitro Digestion of Brassicaceae Feed.” Applied and Environmental Microbiology 80 (23): 7433–7442.
APA
Kiebooms, J., Wauters, J., Vanden Bussche, J., Houf, K., De Vos, P., Van Trappen, S., Cleenwerck, I., et al. (2014). Thiouracil-forming bacteria identified and characterized upon porcine in vitro digestion of Brassicaceae feed. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(23), 7433–7442.
Vancouver
1.
Kiebooms J, Wauters J, Vanden Bussche J, Houf K, De Vos P, Van Trappen S, et al. Thiouracil-forming bacteria identified and characterized upon porcine in vitro digestion of Brassicaceae feed. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2014;80(23):7433–42.
MLA
Kiebooms, Julie, Jella Wauters, Julie Vanden Bussche, et al. “Thiouracil-forming Bacteria Identified and Characterized Upon Porcine in Vitro Digestion of Brassicaceae Feed.” APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.23 (2014): 7433–7442. Print.