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Altered expression of Aurora kinases in Arabidopsis results in aneu- and polyploidization

Dmitri Demidov, Inna Lermontova, Oda Weiss, Joerg Fuchs, Twan Rutten, Katrin Kumke, Timothy F Sharbel, Daniël Van Damme UGent, Nico De Storme UGent, Danny Geelen UGent, et al. (2014) PLANT JOURNAL. 80(3). p.449-461
abstract
Aurora is an evolutionary conserved protein kinase family involved in monitoring of chromosome segregation via phosphorylation of different substrates. In plants, however, the involvement of Aurora proteins in meiosis and in sensing microtubule attachment remains to be proven, although the downstream components leading to the targeting of spindle assembly checkpoint signals to anaphase-promoting complex have been described. To analyze the three members of Aurora family (AtAurora1, -2, and -3) of Arabidopsis we employed different combinations of T-DNA insertion mutants and/or RNAi transformants. Meiotic defects and the formation of unreduced pollen were revealed including plants with an increased ploidy level. The effect of reduced expression of Aurora was mimicked by application of the ATP-competitive Aurora inhibitor II. In addition, strong overexpression of any member of the AtAurora family is not possible. Only tagged or truncated forms of Aurora kinases can be overexpressed. Expression of truncated AtAurora1 resulted in a high number of aneuploids in Arabidopsis, while expression of AtAurora1-TAPi construct in tobacco resulted in 4C (possible tetraploid) progeny. In conclusion, our data demonstrate an essential role of Aurora kinases in the monitoring of meiosis in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MEIOSIS-I, SPINDLE ASSEMBLY CHECKPOINT, HISTONE H3, ANAPHASE-PROMOTING COMPLEX, CELL-CYCLE PROGRESSION, BUDDING YEAST, Arabidopsis thaliana, A OVEREXPRESSION, MOUSE OOCYTES, PASSENGER COMPLEX, chromosome segregation, spindle assembly checkpoint, meiotic defects, aneuploidization, Aurora kinase, polyploidization, CHROMOSOME SEGREGATION
journal title
PLANT JOURNAL
Plant J.
volume
80
issue
3
pages
449 - 461
Web of Science type
Article
Web of Science id
000343877900006
JCR category
PLANT SCIENCES
JCR impact factor
5.972 (2014)
JCR rank
10/204 (2014)
JCR quartile
1 (2014)
ISSN
0960-7412
DOI
10.1111/tpj.12647
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5773146
handle
http://hdl.handle.net/1854/LU-5773146
date created
2014-12-05 11:48:01
date last changed
2016-12-19 15:39:47
@article{5773146,
  abstract     = {Aurora is an evolutionary conserved protein kinase family involved in monitoring of chromosome segregation via phosphorylation of different substrates. In plants, however, the involvement of Aurora proteins in meiosis and in sensing microtubule attachment remains to be proven, although the downstream components leading to the targeting of spindle assembly checkpoint signals to anaphase-promoting complex have been described. To analyze the three members of Aurora family (AtAurora1, -2, and -3) of Arabidopsis we employed different combinations of T-DNA insertion mutants and/or RNAi transformants. Meiotic defects and the formation of unreduced pollen were revealed including plants with an increased ploidy level. The effect of reduced expression of Aurora was mimicked by application of the ATP-competitive Aurora inhibitor II. In addition, strong overexpression of any member of the AtAurora family is not possible. Only tagged or truncated forms of Aurora kinases can be overexpressed. Expression of truncated AtAurora1 resulted in a high number of aneuploids in Arabidopsis, while expression of AtAurora1-TAPi construct in tobacco resulted in 4C (possible tetraploid) progeny. In conclusion, our data demonstrate an essential role of Aurora kinases in the monitoring of meiosis in plants.},
  author       = {Demidov, Dmitri and Lermontova, Inna and Weiss, Oda and Fuchs, Joerg and Rutten, Twan and Kumke, Katrin and Sharbel, Timothy F and Van Damme, Dani{\"e}l and De Storme, Nico and Geelen, Danny and Houben, Andreas},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {MEIOSIS-I,SPINDLE ASSEMBLY CHECKPOINT,HISTONE H3,ANAPHASE-PROMOTING COMPLEX,CELL-CYCLE PROGRESSION,BUDDING YEAST,Arabidopsis thaliana,A OVEREXPRESSION,MOUSE OOCYTES,PASSENGER COMPLEX,chromosome segregation,spindle assembly checkpoint,meiotic defects,aneuploidization,Aurora kinase,polyploidization,CHROMOSOME SEGREGATION},
  language     = {eng},
  number       = {3},
  pages        = {449--461},
  title        = {Altered expression of Aurora kinases in Arabidopsis results in aneu- and polyploidization},
  url          = {http://dx.doi.org/10.1111/tpj.12647},
  volume       = {80},
  year         = {2014},
}

Chicago
Demidov, Dmitri, Inna Lermontova, Oda Weiss, Joerg Fuchs, Twan Rutten, Katrin Kumke, Timothy F Sharbel, et al. 2014. “Altered Expression of Aurora Kinases in Arabidopsis Results in Aneu- and Polyploidization.” Plant Journal 80 (3): 449–461.
APA
Demidov, D., Lermontova, I., Weiss, O., Fuchs, J., Rutten, T., Kumke, K., Sharbel, T. F., et al. (2014). Altered expression of Aurora kinases in Arabidopsis results in aneu- and polyploidization. PLANT JOURNAL, 80(3), 449–461.
Vancouver
1.
Demidov D, Lermontova I, Weiss O, Fuchs J, Rutten T, Kumke K, et al. Altered expression of Aurora kinases in Arabidopsis results in aneu- and polyploidization. PLANT JOURNAL. 2014;80(3):449–61.
MLA
Demidov, Dmitri, Inna Lermontova, Oda Weiss, et al. “Altered Expression of Aurora Kinases in Arabidopsis Results in Aneu- and Polyploidization.” PLANT JOURNAL 80.3 (2014): 449–461. Print.