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TNFR1-induced lethal inflammation is mediated by goblet and Paneth cell dysfunction

Filip Van Hauwermeiren UGent, Roosmarijn Vandenbroucke UGent, LYNDA GRINE, Sofie Lodens UGent, Elien Van Wonterghem UGent, Riet De Rycke UGent, N De Geest, B Hassan and Claude Libert UGent (2015) MUCOSAL IMMUNOLOGY. 8(4). p.828-840
abstract
Tumor necrosis factor (TNF) is a powerful activator of the immune system and a well-validated target for treatment of autoimmune diseases. Injection of TNF induces systemic lethal inflammation characterized by hypothermia, induction of multiple cytokines, and extensive damage to multiple organs. Previously, we reported that TNF-induced lethal inflammation is strictly TNFR1(P55)-dependent. We also uncovered a crucial role for P55 expression levels in intestinal epithelial cells (IECs), in which P55+/+ expression is sufficient to sensitize to TNF lethality in an otherwise fully protected P55+/− background. Here, we investigated the molecular mechanism that drives TNF toxicity in IECs. Unexpectedly, we found that the degree of TNF-induced enterocyte damage and apoptosis in IECs is equally strong in TNF-sensitive P55+/+ mice and TNF-resistant P55+/− mice. Our results suggest that P55+/+-induced signaling causes goblet and Paneth cell dysfunction, leading to severe epithelial barrier dysfunction. As a result, intestinal permeability and systemic bacterial spread are induced, causing lethal systemic inflammation. In conclusion, we identified P55-induced goblet and Paneth cell dysfunction as a crucial mechanism for TNF-induced systemic and lethal inflammation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CHRONIC INTESTINAL INFLAMMATION, TUMOR-NECROSIS-FACTOR, ENDOPLASMIC-RETICULUM STRESS, CROHNS-DISEASE, NECROTIZING ENTEROCOLITIS, ER STRESS, BACTERIAL TRANSLOCATION, TNF-ALPHA, IN-VIVO, SHOCK
journal title
MUCOSAL IMMUNOLOGY
Mucosal Immunol.
volume
8
issue
4
pages
828 - 840
Web of Science type
Article
Web of Science id
000356865700012
JCR category
IMMUNOLOGY
JCR impact factor
6.103 (2015)
JCR rank
23/150 (2015)
JCR quartile
1 (2015)
ISSN
1933-0219
DOI
10.1038/mi.2014.112
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5769461
handle
http://hdl.handle.net/1854/LU-5769461
date created
2014-12-02 17:44:34
date last changed
2016-12-19 15:42:01
@article{5769461,
  abstract     = {Tumor necrosis factor (TNF) is a powerful activator of the immune system and a well-validated target for treatment of autoimmune diseases. Injection of TNF induces systemic lethal inflammation characterized by hypothermia, induction of multiple cytokines, and extensive damage to multiple organs. Previously, we reported that TNF-induced lethal inflammation is strictly TNFR1(P55)-dependent. We also uncovered a crucial role for P55 expression levels in intestinal epithelial cells (IECs), in which P55+/+ expression is sufficient to sensitize to TNF lethality in an otherwise fully protected P55+/\ensuremath{-} background. Here, we investigated the molecular mechanism that drives TNF toxicity in IECs. Unexpectedly, we found that the degree of TNF-induced enterocyte damage and apoptosis in IECs is equally strong in TNF-sensitive P55+/+ mice and TNF-resistant P55+/\ensuremath{-} mice. Our results suggest that P55+/+-induced signaling causes goblet and Paneth cell dysfunction, leading to severe epithelial barrier dysfunction. As a result, intestinal permeability and systemic bacterial spread are induced, causing lethal systemic inflammation. In conclusion, we identified P55-induced goblet and Paneth cell dysfunction as a crucial mechanism for TNF-induced systemic and lethal inflammation.},
  author       = {Van Hauwermeiren, Filip and Vandenbroucke, Roosmarijn and GRINE, LYNDA and Lodens, Sofie and Van Wonterghem, Elien and De Rycke, Riet and De Geest, N and Hassan, B and Libert, Claude},
  issn         = {1933-0219},
  journal      = {MUCOSAL IMMUNOLOGY},
  keyword      = {CHRONIC INTESTINAL INFLAMMATION,TUMOR-NECROSIS-FACTOR,ENDOPLASMIC-RETICULUM STRESS,CROHNS-DISEASE,NECROTIZING ENTEROCOLITIS,ER STRESS,BACTERIAL TRANSLOCATION,TNF-ALPHA,IN-VIVO,SHOCK},
  language     = {eng},
  number       = {4},
  pages        = {828--840},
  title        = {TNFR1-induced lethal inflammation is mediated by goblet and Paneth cell dysfunction},
  url          = {http://dx.doi.org/10.1038/mi.2014.112},
  volume       = {8},
  year         = {2015},
}

Chicago
Van Hauwermeiren, Filip, Roosmarijn Vandenbroucke, LYNDA GRINE, Sofie Lodens, Elien Van Wonterghem, Riet De Rycke, N De Geest, B Hassan, and Claude Libert. 2015. “TNFR1-induced Lethal Inflammation Is Mediated by Goblet and Paneth Cell Dysfunction.” Mucosal Immunology 8 (4): 828–840.
APA
Van Hauwermeiren, F., Vandenbroucke, R., GRINE, L., Lodens, S., Van Wonterghem, E., De Rycke, R., De Geest, N., et al. (2015). TNFR1-induced lethal inflammation is mediated by goblet and Paneth cell dysfunction. MUCOSAL IMMUNOLOGY, 8(4), 828–840.
Vancouver
1.
Van Hauwermeiren F, Vandenbroucke R, GRINE L, Lodens S, Van Wonterghem E, De Rycke R, et al. TNFR1-induced lethal inflammation is mediated by goblet and Paneth cell dysfunction. MUCOSAL IMMUNOLOGY. 2015;8(4):828–40.
MLA
Van Hauwermeiren, Filip, Roosmarijn Vandenbroucke, LYNDA GRINE, et al. “TNFR1-induced Lethal Inflammation Is Mediated by Goblet and Paneth Cell Dysfunction.” MUCOSAL IMMUNOLOGY 8.4 (2015): 828–840. Print.