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Magnetic layer-by-layer coated particles for efficient MRI of dendritic cells and mesenchymal stem cells

Marie-Luce De Temmerman UGent, Stefaan Soenen, Nathalie Symens, Bart Lucas UGent, Roosmarijn Vandenbroucke UGent, Claude Libert UGent, Jo Demeester UGent, Stefaan De Smedt UGent, Uwe Himmelreich and Joanna Rejman UGent (2014) NANOMEDICINE. 9(9). p.1363-1376
abstract
Aim: Cell detection by MRI requires high doses of contrast agent for generating image contrast. Therefore, there is a constant need to develop improved systems that further increase sensitivity, and which could be used in clinical settings. In this study, we devised layer-by-layer particles and tested their potential for cell labeling. Materials & methods: The advantages of layer-by-layer technology were exploited to obtain magnetic particles of controllable size, surface chemistry and magnetic payload. Results: Flexibility in size and surface charge enabled efficient intracellular delivery of magnetic particles in mesenchymal stem cells and dendritic cells. Owing to the high magnetic payload of the particles, high MRI contrast was generated, even for very low cell numbers. Subcutaneous injection of the particles and subsequent uptake by dendritic cells enabled clear visualization of dendritic cells homing towards nearby lymph nodes in mice. Conclusion: The magnetic particles offer several possibilities as efficient cellular MRI contrast agents for direct in vitro or in vivo cell labeling.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
dendritic cell, cell labeling, iron oxide nanoparticle, layer-by-layer, mesenchymal stem cell, MRI, IRON-OXIDE NANOPARTICLES, IN-VIVO, CONTRAST AGENTS, DRUG-DELIVERY, SINGLE CELLS, MOUSE-BRAIN, TRACKING, MAGNETOLIPOSOMES
journal title
NANOMEDICINE
Nanomedicine
volume
9
issue
9
pages
1363 - 1376
Web of Science type
Article
Web of Science id
000342071700013
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
5.413 (2014)
JCR rank
16/163 (2014)
JCR quartile
1 (2014)
ISSN
1743-5889
DOI
10.2217/nnm.13.88
project
NB-Photonics
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
5769380
handle
http://hdl.handle.net/1854/LU-5769380
date created
2014-12-02 16:55:50
date last changed
2017-02-10 15:35:50
@article{5769380,
  abstract     = {Aim: Cell detection by MRI requires high doses of contrast agent for generating image contrast. Therefore, there is a constant need to develop improved systems that further increase sensitivity, and which could be used in clinical settings. In this study, we devised layer-by-layer particles and tested their potential for cell labeling.
Materials \& methods: The advantages of layer-by-layer technology were exploited to obtain magnetic particles of controllable size, surface chemistry and magnetic payload.
Results: Flexibility in size and surface charge enabled efficient intracellular delivery of magnetic particles in mesenchymal stem cells and dendritic cells. Owing to the high magnetic payload of the particles, high MRI contrast was generated, even for very low cell numbers. Subcutaneous injection of the particles and subsequent uptake by dendritic cells enabled clear visualization of dendritic cells homing towards nearby lymph nodes in mice.
Conclusion: The magnetic particles offer several possibilities as efficient cellular MRI contrast agents for direct in vitro or in vivo cell labeling.},
  author       = {De Temmerman, Marie-Luce and Soenen, Stefaan and Symens, Nathalie and Lucas, Bart and Vandenbroucke, Roosmarijn and Libert, Claude and Demeester, Jo and De Smedt, Stefaan and Himmelreich, Uwe and Rejman, Joanna},
  issn         = {1743-5889},
  journal      = {NANOMEDICINE},
  keyword      = {dendritic cell,cell labeling,iron oxide nanoparticle,layer-by-layer,mesenchymal stem cell,MRI,IRON-OXIDE NANOPARTICLES,IN-VIVO,CONTRAST AGENTS,DRUG-DELIVERY,SINGLE CELLS,MOUSE-BRAIN,TRACKING,MAGNETOLIPOSOMES},
  language     = {eng},
  number       = {9},
  pages        = {1363--1376},
  title        = {Magnetic layer-by-layer coated particles for efficient MRI of dendritic cells and mesenchymal stem cells},
  url          = {http://dx.doi.org/10.2217/nnm.13.88},
  volume       = {9},
  year         = {2014},
}

Chicago
De Temmerman, Marie-Luce, Stefaan Soenen, Nathalie Symens, Bart Lucas, Roosmarijn Vandenbroucke, Claude Libert, Jo Demeester, Stefaan De Smedt, Uwe Himmelreich, and Joanna Rejman. 2014. “Magnetic Layer-by-layer Coated Particles for Efficient MRI of Dendritic Cells and Mesenchymal Stem Cells.” Nanomedicine 9 (9): 1363–1376.
APA
De Temmerman, M.-L., Soenen, S., Symens, N., Lucas, B., Vandenbroucke, R., Libert, C., Demeester, J., et al. (2014). Magnetic layer-by-layer coated particles for efficient MRI of dendritic cells and mesenchymal stem cells. NANOMEDICINE, 9(9), 1363–1376.
Vancouver
1.
De Temmerman M-L, Soenen S, Symens N, Lucas B, Vandenbroucke R, Libert C, et al. Magnetic layer-by-layer coated particles for efficient MRI of dendritic cells and mesenchymal stem cells. NANOMEDICINE. 2014;9(9):1363–76.
MLA
De Temmerman, Marie-Luce, Stefaan Soenen, Nathalie Symens, et al. “Magnetic Layer-by-layer Coated Particles for Efficient MRI of Dendritic Cells and Mesenchymal Stem Cells.” NANOMEDICINE 9.9 (2014): 1363–1376. Print.