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Evaluation of sphingomyelin, cholester, and phosphatidylcholine-based immobilized artificial membrane liquid chromatography to predict drug penetration across the blood-brain barrier

Mike De Vrieze (UGent) , Dieter Verzele, Roman Szucs (UGent) , Patrick Sandra (UGent) and Frederic Lynen (UGent)
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Abstract
Over the past decades, several in vitro methods have been tested for their ability to predict drug penetration across the blood-brain barrier. So far, in high-performance liquid chromatography, most attention has been paid to micellar liquid chromatography and immobilized artificial membrane (IAM) LC. IAMLC has been described as a viable approach, since the stationary phase emulates the lipid environment of a cell membrane. However, research in IAMLC has almost exclusively been limited to phosphatidylcholine (PC)-based stationary phases, even though PC is only one of the lipids present in cell membranes. In this article, sphingomyelin and cholester stationary phases have been tested for the first time towards their ability to predict drug penetration across the blood-brain barrier. Upon comparison with the PC stationary phase, the sphingomyelin- and cholester-based columns depict similar predictive performance. Combining data from the different stationary phases did not lead to improvements of the models.
Keywords
PARTITION, MODELS, DESCRIPTORS, RETENTION, PERMEABILITY, IN-SILICO PREDICTION, Cholester, Sphingomyelin, Phosphatidylcholine, Immobilized artificial membrane, Blood-brain barrier, Liquid chromatography

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Chicago
De Vrieze, Mike, Dieter Verzele, Roman Szucs, Patrick Sandra, and Frederic Lynen. 2014. “Evaluation of Sphingomyelin, Cholester, and Phosphatidylcholine-based Immobilized Artificial Membrane Liquid Chromatography to Predict Drug Penetration Across the Blood-brain Barrier.” Analytical and Bioanalytical Chemistry 406 (25): 6179–6188.
APA
De Vrieze, M., Verzele, D., Szucs, R., Sandra, P., & Lynen, F. (2014). Evaluation of sphingomyelin, cholester, and phosphatidylcholine-based immobilized artificial membrane liquid chromatography to predict drug penetration across the blood-brain barrier. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406(25), 6179–6188.
Vancouver
1.
De Vrieze M, Verzele D, Szucs R, Sandra P, Lynen F. Evaluation of sphingomyelin, cholester, and phosphatidylcholine-based immobilized artificial membrane liquid chromatography to predict drug penetration across the blood-brain barrier. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2014;406(25):6179–88.
MLA
De Vrieze, Mike, Dieter Verzele, Roman Szucs, et al. “Evaluation of Sphingomyelin, Cholester, and Phosphatidylcholine-based Immobilized Artificial Membrane Liquid Chromatography to Predict Drug Penetration Across the Blood-brain Barrier.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.25 (2014): 6179–6188. Print.
@article{5768496,
  abstract     = {Over the past decades, several in vitro methods have been tested for their ability to predict drug penetration across the blood-brain barrier. So far, in high-performance liquid chromatography, most attention has been paid to micellar liquid chromatography and immobilized artificial membrane (IAM) LC. IAMLC has been described as a viable approach, since the stationary phase emulates the lipid environment of a cell membrane. However, research in IAMLC has almost exclusively been limited to phosphatidylcholine (PC)-based stationary phases, even though PC is only one of the lipids present in cell membranes. In this article, sphingomyelin and cholester stationary phases have been tested for the first time towards their ability to predict drug penetration across the blood-brain barrier. Upon comparison with the PC stationary phase, the sphingomyelin- and cholester-based columns depict similar predictive performance. Combining data from the different stationary phases did not lead to improvements of the models.},
  author       = {De Vrieze, Mike and Verzele, Dieter and Szucs, Roman and Sandra, Patrick and Lynen, Frederic},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {PARTITION,MODELS,DESCRIPTORS,RETENTION,PERMEABILITY,IN-SILICO PREDICTION,Cholester,Sphingomyelin,Phosphatidylcholine,Immobilized artificial membrane,Blood-brain barrier,Liquid chromatography},
  language     = {eng},
  number       = {25},
  pages        = {6179--6188},
  title        = {Evaluation of sphingomyelin, cholester, and phosphatidylcholine-based immobilized artificial membrane liquid chromatography to predict drug penetration across the blood-brain barrier},
  url          = {http://dx.doi.org/10.1007/s00216-014-8054-7},
  volume       = {406},
  year         = {2014},
}

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