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Host-induced increase in larval sea bass mortality in a gnotobiotic challenge test with Vibrio anguillarum

Xuan Li (UGent) , Tom Defoirdt (UGent) , Qian Yang (UGent) , Stanislas Laureau, Peter Bossier (UGent) and Kristof Dierckens (UGent)
(2014) DISEASES OF AQUATIC ORGANISMS. 108(3). p.211-216
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Abstract
Vibrio anguillarum is the major cause of haemorrhagic septicaemia, vibriosis, which is a severe disease affecting marine fish. In this work, it was found that the mortality of gnotobiotic sea bass larvae challenged with V. anguillarum was dependent on the number of dead fish in the vials at the moment of challenge. Based on this finding, the effect of dead hosts (homogenised sea bass larvae or brine shrimp) on the virulence of V. anguillarum towards sea bass larvae was further investigated. Addition of homogenised hosts led to significantly increased larval mortality of challenged larvae, and this was observed for 3 different V. anguillarum strains, i.e. 43, NB 10 and HI 610. In contrast, the addition of similar levels of tryptone had no effect on mortality. In line with this, the motility of all 3 V. anguillarum strains was significantly increased by the addition of homogenised hosts but not by tryptone. These results suggest that dead hosts increase infectivity of V. anguillarum, not merely by offering nutrients to the bacteria, but also by increasing virulence-associated activities such as motility.
Keywords
Host-pathogen interaction, VIRULENCE FACTORS, IDENTIFICATION, Host-microbe interaction, Vibriosis, MOTILITY, TRANSPORT, CHOLERAE, INVASION, HARVEYI, GENE, BIOSYNTHESIS, COLONIZATION

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Chicago
Li, Xuan, Tom Defoirdt, Qian Yang, Stanislas Laureau, Peter Bossier, and Kristof Dierckens. 2014. “Host-induced Increase in Larval Sea Bass Mortality in a Gnotobiotic Challenge Test with Vibrio Anguillarum.” Diseases of Aquatic Organisms 108 (3): 211–216.
APA
Li, Xuan, Defoirdt, T., Yang, Q., Laureau, S., Bossier, P., & Dierckens, K. (2014). Host-induced increase in larval sea bass mortality in a gnotobiotic challenge test with Vibrio anguillarum. DISEASES OF AQUATIC ORGANISMS, 108(3), 211–216.
Vancouver
1.
Li X, Defoirdt T, Yang Q, Laureau S, Bossier P, Dierckens K. Host-induced increase in larval sea bass mortality in a gnotobiotic challenge test with Vibrio anguillarum. DISEASES OF AQUATIC ORGANISMS. 2014;108(3):211–6.
MLA
Li, Xuan et al. “Host-induced Increase in Larval Sea Bass Mortality in a Gnotobiotic Challenge Test with Vibrio Anguillarum.” DISEASES OF AQUATIC ORGANISMS 108.3 (2014): 211–216. Print.
@article{5768015,
  abstract     = {Vibrio anguillarum is the major cause of haemorrhagic septicaemia, vibriosis, which is a severe disease affecting marine fish. In this work, it was found that the mortality of gnotobiotic sea bass larvae challenged with V. anguillarum was dependent on the number of dead fish in the vials at the moment of challenge. Based on this finding, the effect of dead hosts (homogenised sea bass larvae or brine shrimp) on the virulence of V. anguillarum towards sea bass larvae was further investigated. Addition of homogenised hosts led to significantly increased larval mortality of challenged larvae, and this was observed for 3 different V. anguillarum strains, i.e. 43, NB 10 and HI 610. In contrast, the addition of similar levels of tryptone had no effect on mortality. In line with this, the motility of all 3 V. anguillarum strains was significantly increased by the addition of homogenised hosts but not by tryptone. These results suggest that dead hosts increase infectivity of V. anguillarum, not merely by offering nutrients to the bacteria, but also by increasing virulence-associated activities such as motility.},
  author       = {Li, Xuan and Defoirdt, Tom and Yang, Qian and Laureau, Stanislas and Bossier, Peter and Dierckens, Kristof},
  issn         = {0177-5103},
  journal      = {DISEASES OF AQUATIC ORGANISMS},
  language     = {eng},
  number       = {3},
  pages        = {211--216},
  title        = {Host-induced increase in larval sea bass mortality in a gnotobiotic challenge test with Vibrio anguillarum},
  url          = {http://dx.doi.org/10.3354/dao02722},
  volume       = {108},
  year         = {2014},
}

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